RFX2_CANAL
ID RFX2_CANAL Reviewed; 1111 AA.
AC Q5AMQ6; A0A1D8PLF4;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2017, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=RFX-like DNA-binding protein RFX2;
GN Name=RFX2; OrderedLocusNames=CAALFM_C402030WA;
GN ORFNames=CaO19.12059, CaO19.4590;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP INDUCTION.
RX PubMed=11532938; DOI=10.1093/emboj/20.17.4742;
RA Murad A.M., Leng P., Straffon M., Wishart J., Macaskill S., MacCallum D.,
RA Schnell N., Talibi D., Marechal D., Tekaia F., d'Enfert C., Gaillardin C.,
RA Odds F.C., Brown A.J.;
RT "NRG1 represses yeast-hypha morphogenesis and hypha-specific gene
RT expression in Candida albicans.";
RL EMBO J. 20:4742-4752(2001).
RN [5]
RP INDUCTION.
RX PubMed=18321992; DOI=10.1091/mbc.e07-09-0960;
RA Goyard S., Knechtle P., Chauvel M., Mallet A., Prevost M.C., Proux C.,
RA Coppee J.Y., Schwartz P., Dromer F., Park H., Filler S.G., Janbon G.,
RA d'Enfert C.;
RT "The Yak1 kinase is involved in the initiation and maintenance of hyphal
RT growth in Candida albicans.";
RL Mol. Biol. Cell 19:2251-2266(2008).
RN [6]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=19252121; DOI=10.1128/ec.00246-08;
RA Hao B., Clancy C.J., Cheng S., Raman S.B., Iczkowski K.A., Nguyen M.H.;
RT "Candida albicans RFX2 encodes a DNA binding protein involved in DNA damage
RT responses, morphogenesis, and virulence.";
RL Eukaryot. Cell 8:627-639(2009).
RN [7]
RP INDUCTION.
RX PubMed=19700637; DOI=10.1128/ec.00165-09;
RA Park H., Liu Y., Solis N., Spotkov J., Hamaker J., Blankenship J.R.,
RA Yeaman M.R., Mitchell A.P., Liu H., Filler S.G.;
RT "Transcriptional responses of candida albicans to epithelial and
RT endothelial cells.";
RL Eukaryot. Cell 8:1498-1510(2009).
RN [8]
RP INDUCTION.
RX PubMed=20808890; DOI=10.1371/journal.pgen.1001070;
RA Tuch B.B., Mitrovich Q.M., Homann O.R., Hernday A.D., Monighetti C.K.,
RA De La Vega F.M., Johnson A.D.;
RT "The transcriptomes of two heritable cell types illuminate the circuit
RT governing their differentiation.";
RL PLoS Genet. 6:E1001070-E1001070(2010).
RN [9]
RP INDUCTION.
RX PubMed=21257795; DOI=10.1128/ec.00278-10;
RA Hussein B., Huang H., Glory A., Osmani A., Kaminskyj S., Nantel A.,
RA Bachewich C.;
RT "G1/S transcription factor orthologues Swi4p and Swi6p are important but
RT not essential for cell proliferation and influence hyphal development in
RT the fungal pathogen Candida albicans.";
RL Eukaryot. Cell 10:384-397(2011).
CC -!- FUNCTION: Transcriptional repressor which regulates DNA damage
CC responses, morphogenesis, and virulence. Involved in the regulation of
CC filamentous growth through its repression of hyphal-specific genes such
CC as HWP1, ALS3, HYR1, ECE1, and CEK1. {ECO:0000269|PubMed:19252121}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00858}.
CC -!- INDUCTION: Expression is negatively regulated by NRG1, and by the
CC contact with host oral epithelial cells. Up-regulated during the yeast-
CC to-hypha transition through the function of YAK1. Also regulated by
CC SWI4, SWI6, and WOR1, a transcriptional regulator of white-opaque
CC switching. {ECO:0000269|PubMed:11532938, ECO:0000269|PubMed:18321992,
CC ECO:0000269|PubMed:19700637, ECO:0000269|PubMed:20808890,
CC ECO:0000269|PubMed:21257795}.
CC -!- DISRUPTION PHENOTYPE: Leads to increased resistance to UV-killing,
CC derepression of various stress-related genes, hyperfilamentous growth,
CC and overexpression of hypha-specific genes.
CC {ECO:0000269|PubMed:19252121}.
CC -!- SIMILARITY: Belongs to the RFX family. {ECO:0000255|PROSITE-
CC ProRule:PRU00858}.
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DR EMBL; CP017626; AOW28974.1; -; Genomic_DNA.
DR RefSeq; XP_722662.2; XM_717569.2.
DR AlphaFoldDB; Q5AMQ6; -.
DR PRIDE; Q5AMQ6; -.
DR GeneID; 3635658; -.
DR KEGG; cal:CAALFM_C402030WA; -.
DR CGD; CAL0000192725; RFX2.
DR VEuPathDB; FungiDB:C4_02030W_A; -.
DR HOGENOM; CLU_009415_0_0_1; -.
DR InParanoid; Q5AMQ6; -.
DR OMA; LYNSAFR; -.
DR OrthoDB; 346630at2759; -.
DR PRO; PR:Q5AMQ6; -.
DR Proteomes; UP000000559; Chromosome 4.
DR GO; GO:0032021; C:NELF complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:CGD.
DR GO; GO:0044406; P:adhesion of symbiont to host; IMP:CGD.
DR GO; GO:0034605; P:cellular response to heat; IMP:CGD.
DR GO; GO:0034599; P:cellular response to oxidative stress; IMP:CGD.
DR GO; GO:0034644; P:cellular response to UV; IMP:CGD.
DR GO; GO:0030447; P:filamentous growth; IMP:CGD.
DR GO; GO:0044182; P:filamentous growth of a population of unicellular organisms; IMP:CGD.
DR GO; GO:1900429; P:negative regulation of filamentous growth of a population of unicellular organisms; IMP:CGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:CGD.
DR GO; GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR GO; GO:0010768; P:negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage; IMP:CGD.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR003150; DNA-bd_RFX.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF02257; RFX_DNA_binding; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS51526; RFX_DBD; 1.
PE 2: Evidence at transcript level;
KW DNA damage; DNA-binding; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation; Virulence.
FT CHAIN 1..1111
FT /note="RFX-like DNA-binding protein RFX2"
FT /id="PRO_0000424608"
FT DNA_BIND 450..540
FT /note="RFX-type winged-helix"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00858"
FT REGION 1..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 300..320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 570..642
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 925..988
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1050..1076
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..111
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 129..199
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 300..314
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 570..590
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 945..981
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1111 AA; 125295 MW; D8F4753347466F65 CRC64;
MSNNPHRTHK RQKSSVSNPG YYFTPETKSE IQQQQPQQQE SQQQQQQQQQ QHSQTHNIYD
NDNYMNYNFP PTSNRPRAST TTGTTSTTHP GSELSHESHS VHTSPLKRTA SSELDQPIPA
MAPSSPLVSS APYYYQQPSQ QQNLSYHDHH HQQQQQQSTP QGQQLSQQTQ SNSQSGVPPP
LYGTSSSIPP GSTMQPSTSF AFHTSHSTYN PSFDSSNLYN SAFRLPEYPT TSSSSLLSTT
GGKQFQQSSA LLPSGTLPPS ILGTSSSSHV SALRQHQKNN LSISSHLTLF SLSGNNSSQL
QSQGSSFQQS ETGVDDTKRS SKESTTIFND LLFHLTSVDG SNINTFLLSI LRKINSPFTL
DDFYNLLYND RQRTLLDNSN YQNRIDKTIV SPSDTDMTVS IINQLLNFFK TPSMLVDYFP
NMEDKDNKLA NINYHELLRT FLAIKILHDI LIQLPISEDD DPQNYTIPRL SIYKTYYIIC
QKLIASYPSA SNTRNEQQKL ILGQSKLGKL IKLVYPNLLI KRLGSRGESK YNYLGVMWNA
NIVQEEIKQL CDEHELNDLN EIFNSDNNNP FASIAPSGSA TTGSTPRRGL SHKRTSSKQK
IKTEPVAGNP FLQPLQTSHH HHHSHHHHHE EQSQEESLSQ QMGEHITAPR LSFLRANSKY
PTDVNLSVLD DDNWFVRLSY ECYARQPALN RDLIQQIFLK NEFLLNNSSL LQNLMDSIIK
PLVMQETYSN VDLVLYLAIL LEILPYLLLV KSSTNINLLK NLRLNLLHLI NNFNNELKKL
DSPKFPIERS TIFLVLVKKL INLNDLLITF IKLINRDNCK TTMSSDIENF LKINSQTVKL
DDDDNSFFFN LNTTSMGEVN FNFKNEILSN DLIYTLIGYN FDPTTNSELK SSISMNFINE
EINVIDEFFK NDLLNFLSTD FHAGLDDDNG EEDDDEEAGP GSTHPMGATG SQGSLSPEPV
STGNPSVPPT RNTSISEVNA ENKRGNEAVL TPKETAKLNS LISLIDKRLL SSQFKSKYPI
LMYNNCISYI LNDILKHIFL KQQQQQLQSS SSQLHDTQPL TQQDTAQGIG SSSSNANNTN
SSFGNWWVFN SFIQEYMSLI GELVGLHDNL V
