RFX2_DANRE
ID RFX2_DANRE Reviewed; 734 AA.
AC Q5EAP5;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=DNA-binding protein RFX2;
DE AltName: Full=Regulatory factor X 2;
GN Name=rfx2; ORFNames=zgc:91808;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=22233545; DOI=10.1016/j.ydbio.2011.12.030;
RA Bisgrove B.W., Makova S., Yost H.J., Brueckner M.;
RT "RFX2 is essential in the ciliated organ of asymmetry and an RFX2 transgene
RT identifies a population of ciliated cells sufficient for fluid flow.";
RL Dev. Biol. 363:166-178(2012).
CC -!- FUNCTION: Transcription factor that acts as a key regulator of
CC ciliogenesis (PubMed:22233545). Specifically regulates expression of
CC genes required for cilium assembly and function (PubMed:22233545).
CC Recognizes and binds the X-box, a regulatory motif with DNA sequence
CC 5'-GTNRCC(0-3N)RGYAAC-3' present on promoters (By similarity).
CC {ECO:0000250|UniProtKB:P48379, ECO:0000269|PubMed:22233545}.
CC -!- SUBUNIT: Homodimer. Heterodimer; heterodimerizes with other rfx
CC proteins. {ECO:0000250|UniProtKB:P48379}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:B2GV50,
CC ECO:0000255|PROSITE-ProRule:PRU00858}. Cytoplasm
CC {ECO:0000250|UniProtKB:B2GV50}. Note=Mainly expressed in the nucleus
CC and at lower level in cytoplasm. {ECO:0000250|UniProtKB:B2GV50}.
CC -!- DISRUPTION PHENOTYPE: Reduced cilia length in the Kupffer's vesicle and
CC impaired left-right asymmetry. {ECO:0000269|PubMed:22233545}.
CC -!- SIMILARITY: Belongs to the RFX family. {ECO:0000255|PROSITE-
CC ProRule:PRU00858}.
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DR EMBL; BC090314; AAH90314.1; -; mRNA.
DR RefSeq; NP_001013296.1; NM_001013278.1.
DR AlphaFoldDB; Q5EAP5; -.
DR SMR; Q5EAP5; -.
DR STRING; 7955.ENSDARP00000108057; -.
DR PaxDb; Q5EAP5; -.
DR PRIDE; Q5EAP5; -.
DR Ensembl; ENSDART00000029939; ENSDARP00000039332; ENSDARG00000013575.
DR GeneID; 503590; -.
DR KEGG; dre:503590; -.
DR CTD; 5990; -.
DR ZFIN; ZDB-GENE-050227-4; rfx2.
DR eggNOG; KOG3712; Eukaryota.
DR GeneTree; ENSGT01050000244879; -.
DR HOGENOM; CLU_010393_1_1_1; -.
DR InParanoid; Q5EAP5; -.
DR OMA; QYMAIRQ; -.
DR OrthoDB; 346630at2759; -.
DR PhylomeDB; Q5EAP5; -.
DR TreeFam; TF321340; -.
DR PRO; PR:Q5EAP5; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 8.
DR Bgee; ENSDARG00000013575; Expressed in testis and 79 other tissues.
DR ExpressionAtlas; Q5EAP5; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:ZFIN.
DR GO; GO:0070121; P:Kupffer's vesicle development; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR003150; DNA-bd_RFX.
DR InterPro; IPR039779; RFX-like.
DR InterPro; IPR007668; RFX1_trans_act.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12619; PTHR12619; 1.
DR Pfam; PF04589; RFX1_trans_act; 1.
DR Pfam; PF02257; RFX_DNA_binding; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS51526; RFX_DBD; 1.
PE 2: Evidence at transcript level;
KW Cilium biogenesis/degradation; Cytoplasm; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..734
FT /note="DNA-binding protein RFX2"
FT /id="PRO_0000380697"
FT DNA_BIND 204..279
FT /note="RFX-type winged-helix"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00858"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 694..722
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 705..722
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 734 AA; 81854 MW; 7CF2543BF45BDF11 CRC64;
MQRSEGGSET PSTVALRTST SAQAPVVQPV PASQQRVLVQ ATGSSQKGAQ VQQLPVPRVQ
QVPQQVQQVQ HYHSQQVQYV EGAEAVYPNG TIRAAYSYNP ESQLYGQGSG SAYFDSQAGG
AQVTTVVSSG GVPTHGMVGI AMDVGGSQII SSGSAYLIHG GMEGGRHHTS HSSRSSSAML
EMAIENLQKS EGIATHKSSL LNSHLQWLLD NYETAEGVSL PRSSLYNHYL RHCQEQKLDP
VNAASFGKLI RSVFMGLRTR RLGTRGNSKY HYYGIRLKPD SPLNRMQEDT QYMAMRQTPV
HQKQRFKPFH KMDGMGDSLN SGSQHLSSTP EQSVAAQCQH HQQYIDVSHV LPPFPSLDLG
SCPLPESISM TDVKKLQSSY RIHCEATLDV VMNLQFHLIE KLWQTFWHST APSSDGATTI
PNSEEDVEDI RGFPRDKLIT LCKYEPIKQW MRSCDHILYQ ALVEILIPDV LRPVPSTLTQ
AIRNFAKSLE GWLTSAMTNF PPEIISTKAA VVSAFAQTLR RYTSLNHLAQ AARAVLQNTS
QINQMLSDLN RVDFANVQEQ ASWVCQCDES VVQRLELDFK ATLQQQSSLD QWAAWLDNVV
NQVLKPYDGS LSFPRAARQF LLKWSFYSSM VIRDLTLRSA ASFGSFHLIR LLYDEYMFYL
VEHRVAQATG ETPIAVMGEF SDLSSLMPSL KEQDTSFSDD MTSDGDMSRM SERSLTEPAV
KRERIEIPHS LQEI
