ID   RFX2_PONAB              Reviewed;         704 AA.
AC   Q5RDR2;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=DNA-binding protein RFX2;
DE   AltName: Full=Regulatory factor X 2;
GN   Name=RFX2;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcription factor that acts as a key regulator of
CC       spermatogenesis. Acts by regulating expression of genes required for
CC       the haploid phase during spermiogenesis, such as genes required for
CC       cilium assembly and function. Recognizes and binds the X-box, a
CC       regulatory motif with DNA sequence 5'-GTNRCC(0-3N)RGYAAC-3' present on
CC       promoters. Probably activates transcription of the testis-specific
CC       histone gene H1-6. {ECO:0000250|UniProtKB:P48379}.
CC   -!- SUBUNIT: Homodimer; probably only forms homodimers in testis.
CC       Heterodimer; heterodimerizes with RFX1 and RFX3.
CC       {ECO:0000250|UniProtKB:P48379}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:B2GV50,
CC       ECO:0000255|PROSITE-ProRule:PRU00858}. Cytoplasm
CC       {ECO:0000250|UniProtKB:B2GV50}. Note=Mainly expressed in the nucleus
CC       and at lower level in cytoplasm. {ECO:0000250|UniProtKB:B2GV50}.
CC   -!- SIMILARITY: Belongs to the RFX family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00858}.
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DR   EMBL; CR857840; CAH90095.1; -; mRNA.
DR   RefSeq; NP_001125003.1; NM_001131531.1.
DR   AlphaFoldDB; Q5RDR2; -.
DR   SMR; Q5RDR2; -.
DR   STRING; 9601.ENSPPYP00000010591; -.
DR   GeneID; 100171882; -.
DR   KEGG; pon:100171882; -.
DR   CTD; 5990; -.
DR   eggNOG; KOG3712; Eukaryota.
DR   InParanoid; Q5RDR2; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR   GO; GO:0001675; P:acrosome assembly; ISS:UniProtKB.
DR   GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0007286; P:spermatid development; ISS:UniProtKB.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR003150; DNA-bd_RFX.
DR   InterPro; IPR039779; RFX-like.
DR   InterPro; IPR007668; RFX1_trans_act.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR12619; PTHR12619; 1.
DR   Pfam; PF04589; RFX1_trans_act; 1.
DR   Pfam; PF02257; RFX_DNA_binding; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS51526; RFX_DBD; 1.
PE   2: Evidence at transcript level;
KW   Cilium biogenesis/degradation; Cytoplasm; Differentiation; DNA-binding;
KW   Nucleus; Phosphoprotein; Reference proteome; Spermatogenesis;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..704
FT                   /note="DNA-binding protein RFX2"
FT                   /id="PRO_0000380695"
FT   DNA_BIND        199..274
FT                   /note="RFX-type winged-helix"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00858"
FT   REGION          1..39
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          292..334
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        307..334
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         28
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P48378"
FT   MOD_RES         416
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:B2GV50"
SQ   SEQUENCE   704 AA;  77621 MW;  70B56E4E4212BBA6 CRC64;
     MQNSEGGADS PASVALRPSA AAPPVPASPQ RVLVQAASSA PKGAQMQPIS LPRVQQVPQQ
     VQPVQHVYPA QVQYAEGGDA VYTNGAIRTA YTYNPEPQMY APSSAASYFE APGGAQVTVA
     ASSPPAVPSH SMVGITMDVG GSPIVSSTGA YLIHGGMDST RHSLAHTSRS SPATLEMAIE
     NLQKSEGITS HKSGLLNSHL QWLLDNYETA EGVSLPRSSL YNHYLRHCQE HKLDPVNAAS
     FGKLIRSVFM GLRTRRLGTR GNSKYHYYGI RLKPDSPLNR LQEDTQYMAM RQQPMHQKPR
     YRPAQKTDSL GDSSSHSGLH STPEQTTAAQ NQHHQQYIDV SHVFPEFPAP DLGSVLLQDG
     VTLHDVKALQ LVYRRHCEAT VDVVMNLQFH YIEKLWLSFW NSKASSSDGP TSLPASDEDP
     EGAVLPKDKL ISLCQCDPIL RWMRSCDHIL YQALVEILIP DVLRPVPSTL TQAIRNFAKS
     LEGWLTNAMS DFPQQVIQTK VGVVSAFAQT LRRYTSLNHL AQAARAVLQN TSQINQMLSD
     LNRVDFANVQ EQASWVCQCE ESVVQRLEQD FKLTLQQQSS LDQWASWLDS VVTQVLKQHA
     GSPSFPKAAR QFLLKWSFYS SMVIRDLTLR SAASFGSFHL IRLLYDEYMF YLVEHRVAEA
     TGETPIAVMG ERLSQNVTSA LWPGSEPGSS PGELCSSAWL ATLC