RFX2_RAT
ID RFX2_RAT Reviewed; 692 AA.
AC B2GV50;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=DNA-binding protein RFX2;
DE AltName: Full=Regulatory factor X 2;
GN Name=Rfx2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=15229132; DOI=10.1095/biolreprod.104.032268;
RA Horvath G.C., Kistler W.S., Kistler M.K.;
RT "RFX2 is a potential transcriptional regulatory factor for histone H1t and
RT other genes expressed during the meiotic phase of spermatogenesis.";
RL Biol. Reprod. 71:1551-1559(2004).
RN [4]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=14743396; DOI=10.1002/jcb.10748;
RA Wolfe S.A., Wilkerson D.C., Prado S., Grimes S.R.;
RT "Regulatory factor X2 (RFX2) binds to the H1t/TE1 promoter element and
RT activates transcription of the testis-specific histone H1t gene.";
RL J. Cell. Biochem. 91:375-383(2004).
RN [5]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=15526285; DOI=10.1002/jcb.20320;
RA Grimes S.R., Prado S., Wolfe S.A.;
RT "Transcriptional activation of the testis-specific histone H1t gene by RFX2
RT may require both proximal promoter X-box elements.";
RL J. Cell. Biochem. 94:317-326(2005).
RN [6]
RP FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=16676351; DOI=10.1002/jcb.20959;
RA Wolfe S.A., van Wert J., Grimes S.R.;
RT "Transcription factor RFX2 is abundant in rat testis and enriched in nuclei
RT of primary spermatocytes where it appears to be required for transcription
RT of the testis-specific histone H1t gene.";
RL J. Cell. Biochem. 99:735-746(2006).
RN [7]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=18247329; DOI=10.1002/jcb.21694;
RA VanWert J.M., Wolfe S.A., Grimes S.R.;
RT "Binding of RFX2 and NF-Y to the testis-specific histone H1t promoter may
RT be required for transcriptional activation in primary spermatocytes.";
RL J. Cell. Biochem. 104:1087-1101(2008).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-386, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Transcription factor that acts as a key regulator of
CC spermatogenesis (By similarity). Acts by regulating expression of genes
CC required for the haploid phase during spermiogenesis, such as genes
CC required for cilium assembly and function (By similarity). Recognizes
CC and binds the X-box, a regulatory motif with DNA sequence 5'-GTNRCC(0-
CC 3N)RGYAAC-3' present on promoters (PubMed:14743396, PubMed:15526285,
CC PubMed:16676351, PubMed:18247329). Probably activates transcription of
CC the testis-specific histone gene H1-6 (PubMed:14743396,
CC PubMed:15526285, PubMed:16676351, PubMed:18247329).
CC {ECO:0000250|UniProtKB:P48379, ECO:0000269|PubMed:14743396,
CC ECO:0000269|PubMed:15526285, ECO:0000269|PubMed:16676351,
CC ECO:0000269|PubMed:18247329}.
CC -!- SUBUNIT: Homodimer; probably only forms homodimers in testis.
CC Heterodimer; heterodimerizes with RFX1 and RFX3.
CC {ECO:0000250|UniProtKB:P48379}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00858,
CC ECO:0000269|PubMed:16676351}. Cytoplasm {ECO:0000269|PubMed:16676351}.
CC Note=Mainly expressed in the nucleus and at lower level in cytoplasm.
CC {ECO:0000269|PubMed:16676351}.
CC -!- TISSUE SPECIFICITY: Expressed at highest level in testis. Expressed at
CC lower level in thymus. Also expressed in stomach, kidney, liver, brain
CC and heart. Weakly expressed in spleen and lung (PubMed:16676351).
CC Within testis, most abundantly present in spermatocytes: present from
CC pachytene spermatocytes to early spermatids (at protein level)
CC (PubMed:15229132, PubMed:16676351). Also present in non-germinal
CC tissues (PubMed:16676351). {ECO:0000269|PubMed:15229132,
CC ECO:0000269|PubMed:16676351}.
CC -!- SIMILARITY: Belongs to the RFX family. {ECO:0000255|PROSITE-
CC ProRule:PRU00858}.
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DR EMBL; CH474092; EDL83603.1; -; Genomic_DNA.
DR EMBL; BC166527; AAI66527.1; -; mRNA.
DR RefSeq; NP_001100347.1; NM_001106877.1.
DR AlphaFoldDB; B2GV50; -.
DR SMR; B2GV50; -.
DR STRING; 10116.ENSRNOP00000064610; -.
DR iPTMnet; B2GV50; -.
DR PhosphoSitePlus; B2GV50; -.
DR PaxDb; B2GV50; -.
DR PRIDE; B2GV50; -.
DR Ensembl; ENSRNOT00000073025; ENSRNOP00000064610; ENSRNOG00000045846.
DR GeneID; 301121; -.
DR KEGG; rno:301121; -.
DR CTD; 5990; -.
DR RGD; 1588579; Rfx2.
DR eggNOG; KOG3712; Eukaryota.
DR GeneTree; ENSGT01050000244879; -.
DR HOGENOM; CLU_010393_1_1_1; -.
DR InParanoid; B2GV50; -.
DR PhylomeDB; B2GV50; -.
DR PRO; PR:B2GV50; -.
DR Proteomes; UP000002494; Chromosome 9.
DR Proteomes; UP000234681; Chromosome 9.
DR Bgee; ENSRNOG00000045846; Expressed in testis and 18 other tissues.
DR ExpressionAtlas; B2GV50; baseline and differential.
DR Genevisible; B2GV50; RN.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR GO; GO:0001675; P:acrosome assembly; ISS:UniProtKB.
DR GO; GO:1990830; P:cellular response to leukemia inhibitory factor; ISO:RGD.
DR GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0007286; P:spermatid development; ISS:UniProtKB.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR003150; DNA-bd_RFX.
DR InterPro; IPR039779; RFX-like.
DR InterPro; IPR007668; RFX1_trans_act.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12619; PTHR12619; 1.
DR Pfam; PF04589; RFX1_trans_act; 1.
DR Pfam; PF02257; RFX_DNA_binding; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS51526; RFX_DBD; 1.
PE 1: Evidence at protein level;
KW Cilium biogenesis/degradation; Cytoplasm; Differentiation; DNA-binding;
KW Nucleus; Phosphoprotein; Reference proteome; Spermatogenesis;
KW Transcription; Transcription regulation.
FT CHAIN 1..692
FT /note="DNA-binding protein RFX2"
FT /id="PRO_0000380696"
FT DNA_BIND 169..244
FT /note="RFX-type winged-helix"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00858"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 261..296
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 660..692
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 280..296
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 660..686
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 26
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P48378"
FT MOD_RES 386
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 692 AA; 76537 MW; AB5EA2C1105A8017 CRC64;
MQNSEGGADS PATVALRPAA QPVPASPQRV LVQAAGSTPK GTPMQTLTLP RVQPVPPQVQ
HVYPAQVQYV EGGDAVYANG AIRAAYTYNP DPQLYAPSSA ASYFETPGGA QVTVAASSPP
AVPSHGMVGI TMDVSGTPIV SGAGTYLIHG GMDSTRHSLA HTARSSPATL QWLLDNYETA
EGVSLPRSSL YNHYLRHCQE HKLEPVNAAS FGKLIRSVFM GLRTRRLGTR GNSKYHYYGI
RLKPDSPLNR LQEDTQYMAM RQQPTHQKPR YRPAQKSDSL GDGSAHSNMH STPEQAMAAQ
GQHHQQYIDV SHVFPEFPAP DLGSTLLQES VTLHDVKALQ LVYRRHCEAT LDVVMNLQFQ
YIEKLWLSFW NCKATSSDGR ASLPASDEEP EVTLLPKDKL ISLCKCEPIL QWMRSCDHIL
YQALVETLIP DVLRPVPSSL TQAIRNFAKS LEGWLINAMS GFPQQVIQTK VGVVSAFAQT
LRRYTSLNHL AQAARAVLQN TSQINQMLSD LNRVDFANVQ EQASWVCQCE ESLVQRLEHD
FKVTLQQQSS LDQWASWLDN VVTQVLKQHA GSPSFPKAAR QFLLKWSFYS SMVIRDLTLR
SAASFGSFHL IRLLYDEYMF YLVEHRVAQA TGETPIAVMG EFNDLASLSL TLLDKEDIGD
GHSSEADVDG RSLGEPLVKR ERSDPSHPLQ GI
