ID   RFX2_XENLA              Reviewed;         694 AA.
AC   Q32NR3;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=DNA-binding protein RFX2;
DE   AltName: Full=Regulatory factor X 2;
GN   Name=rfx2;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=22227339; DOI=10.1016/j.ydbio.2011.12.029;
RA   Chung M.I., Peyrot S.M., LeBoeuf S., Park T.J., McGary K.L., Marcotte E.M.,
RA   Wallingford J.B.;
RT   "RFX2 is broadly required for ciliogenesis during vertebrate development.";
RL   Dev. Biol. 363:155-165(2012).
RN   [3]
RP   FUNCTION.
RX   PubMed=24424412; DOI=10.7554/elife.01439;
RA   Chung M.I., Kwon T., Tu F., Brooks E.R., Gupta R., Meyer M., Baker J.C.,
RA   Marcotte E.M., Wallingford J.B.;
RT   "Coordinated genomic control of ciliogenesis and cell movement by RFX2.";
RL   Elife 3:E01439-E01439(2014).
CC   -!- FUNCTION: Transcription factor that acts as a key regulator of
CC       ciliogenesis. Specifically regulates expression of genes required for
CC       cilium assembly and function. Recognizes and binds the X-box, a
CC       regulatory motif with DNA sequence 5'-GTNRCC(0-3N)RGYAAC-3' present on
CC       promoters (PubMed:22227339, PubMed:24424412). Required for neural tube
CC       closure and neural ciliogenesis (PubMed:22227339).
CC       {ECO:0000269|PubMed:22227339, ECO:0000269|PubMed:24424412}.
CC   -!- SUBUNIT: Homodimer. Heterodimer; heterodimerizes with other rfx
CC       proteins. {ECO:0000250|UniProtKB:P48379}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:B2GV50,
CC       ECO:0000255|PROSITE-ProRule:PRU00858}. Cytoplasm
CC       {ECO:0000250|UniProtKB:B2GV50}. Note=Mainly expressed in the nucleus
CC       and at lower level in cytoplasm. {ECO:0000250|UniProtKB:B2GV50}.
CC   -!- TISSUE SPECIFICITY: Preferentially expressed in ciliated tissues, such
CC       as neural tube, gastrocoel roof plate, epidermal multiciliated cells,
CC       otic vesicles and kidneys. {ECO:0000269|PubMed:22227339}.
CC   -!- SIMILARITY: Belongs to the RFX family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00858}.
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DR   EMBL; BC108517; AAI08518.1; -; mRNA.
DR   RefSeq; NP_001090132.1; NM_001096663.1.
DR   AlphaFoldDB; Q32NR3; -.
DR   SMR; Q32NR3; -.
DR   DNASU; 735210; -.
DR   GeneID; 735210; -.
DR   KEGG; xla:735210; -.
DR   CTD; 735210; -.
DR   Xenbase; XB-GENE-991777; rfx2.S.
DR   OrthoDB; 346630at2759; -.
DR   Proteomes; UP000186698; Chromosome 1S.
DR   Bgee; 735210; Expressed in testis and 19 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:UniProtKB.
DR   GO; GO:0060271; P:cilium assembly; IDA:UniProtKB.
DR   GO; GO:0001843; P:neural tube closure; IMP:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR003150; DNA-bd_RFX.
DR   InterPro; IPR039779; RFX-like.
DR   InterPro; IPR007668; RFX1_trans_act.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR12619; PTHR12619; 2.
DR   Pfam; PF04589; RFX1_trans_act; 1.
DR   Pfam; PF02257; RFX_DNA_binding; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS51526; RFX_DBD; 1.
PE   2: Evidence at transcript level;
KW   Cilium biogenesis/degradation; Cytoplasm; DNA-binding; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..694
FT                   /note="DNA-binding protein RFX2"
FT                   /id="PRO_0000380698"
FT   DNA_BIND        174..249
FT                   /note="RFX-type winged-helix"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00858"
FT   REGION          267..310
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          658..694
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        286..310
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        676..694
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   694 AA;  77784 MW;  5B0E1730A920F24D CRC64;
     MQNSDSGSDS ATSVALRTST SAQAPVVQPV PASQQVQTVQ HVYPAQVQYV EGDTVYTNGA
     IRTAYSYNAE TQIYAPSSGS SYFDSQGGGA QVTTVVSSPT AIPSHSMVGI TMDVSGSQII
     SSSGAYLIHG GLENSRHTPS HTSRTFPATL EMAIENLQKN EGITSHKSSL LNSHLQWLLD
     NYETAEGVSL PRSSLYNHYL RHCQDHKLDP VNAASFGKLI RSVFMGLRTR RLGTRGNSKY
     HYYGIRLKPD SPLNRIQEDA QYMAIRQQPI HQKQRYRPAQ KIDGMGENPA NSSQHASPEQ
     SVAAQSQHHQ QFIDTSHVFP DFPAPDLGSL LLPEGITMTD IKNLQLMYRR HCEATIDVVM
     NLQFQYIEKL WQAFWNSKPS SPDGSNPMSS EDEQEPIIPN EKLMVLCKYE PIMRWMRNCD
     HILYQALVEI LIPDVLRPVP STLTQAIRNF AKSLEGWLTN AMCDFPQQIV HAKVGVVSAF
     AQTLRRYTSL NHLAQAARAV LQNTSQINQM LSDLNRVDFA NVQEQASWVC QCDEGMVQKL
     EQDFKLTLQQ QSSLDQWANW LDNVVTQVLK PHEGSTSFPK AARQFLLKWS FYSSMVIRDL
     TLRSAASFGS FHLIRLLYDE YMFYLVEHRV AQATGETPIA VMGEFSDFAS MSPVQMDKDD
     VSELGSDTEG DPHISGQPPV KRERVELNHS MQEM