ID   RFX2_XENTR              Reviewed;         694 AA.
AC   B1WAV2;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=DNA-binding protein RFX2;
DE   AltName: Full=Regulatory factor X 2;
GN   Name=rfx2;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcription factor that acts as a key regulator of
CC       ciliogenesis. Specifically regulates expression of genes required for
CC       cilium assembly and function. Recognizes and binds the X-box, a
CC       regulatory motif with DNA sequence 5'-GTNRCC(0-3N)RGYAAC-3' present on
CC       promoters. Required for neural tube closure and neural ciliogenesis.
CC       {ECO:0000250|UniProtKB:Q32NR3}.
CC   -!- SUBUNIT: Homodimer. Heterodimer; heterodimerizes with other rfx
CC       proteins. {ECO:0000250|UniProtKB:P48379}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:B2GV50,
CC       ECO:0000255|PROSITE-ProRule:PRU00858}. Cytoplasm
CC       {ECO:0000250|UniProtKB:B2GV50}. Note=Mainly expressed in the nucleus
CC       and at lower level in cytoplasm. {ECO:0000250|UniProtKB:B2GV50}.
CC   -!- SIMILARITY: Belongs to the RFX family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00858}.
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DR   EMBL; BC161512; AAI61512.1; -; mRNA.
DR   RefSeq; NP_001116955.1; NM_001123483.1.
DR   AlphaFoldDB; B1WAV2; -.
DR   SMR; B1WAV2; -.
DR   STRING; 8364.ENSXETP00000047352; -.
DR   PaxDb; B1WAV2; -.
DR   Ensembl; ENSXETT00000012261; ENSXETP00000012261; ENSXETG00000021877.
DR   GeneID; 100144734; -.
DR   KEGG; xtr:100144734; -.
DR   CTD; 5990; -.
DR   Xenbase; XB-GENE-991774; rfx2.
DR   eggNOG; KOG3712; Eukaryota.
DR   HOGENOM; CLU_010393_1_1_1; -.
DR   InParanoid; B1WAV2; -.
DR   OrthoDB; 346630at2759; -.
DR   Proteomes; UP000008143; Chromosome 1.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000021877; Expressed in testis and 21 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR   GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR   GO; GO:0001843; P:neural tube closure; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR003150; DNA-bd_RFX.
DR   InterPro; IPR039779; RFX-like.
DR   InterPro; IPR007668; RFX1_trans_act.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR12619; PTHR12619; 2.
DR   Pfam; PF04589; RFX1_trans_act; 1.
DR   Pfam; PF02257; RFX_DNA_binding; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS51526; RFX_DBD; 1.
PE   2: Evidence at transcript level;
KW   Cilium biogenesis/degradation; Cytoplasm; DNA-binding; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..694
FT                   /note="DNA-binding protein RFX2"
FT                   /id="PRO_0000380699"
FT   DNA_BIND        174..249
FT                   /note="RFX-type winged-helix"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00858"
FT   REGION          268..309
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          659..694
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        285..309
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        676..694
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   694 AA;  77857 MW;  98EA6866EB1002A8 CRC64;
     MQNSDSGSDS ATSVALRTSA SAQAPVVQPV PASQQVQTVQ HVYPAQVQYV EGDTVYTNGA
     IRTAYSYNAE AQIYAPSSGS SYFDSQGGGA QVTTVVSSPT AIPSHSMVGI AMDVSGSQII
     SSSGAYLIHG GLENSRHTPS HTSRTFPATL EMAIENLQKN EGITSHKSSL LNSHLQWLLD
     NYETAEGVSL PRSSLYNHYL RHCQDHKLDP VNAASFGKLI RSVFMGLRTR RLGTRGNSKY
     HYYGIRLKPD SPLNRLQEDT QYMAIRQQPI HQKQRYRPAQ KMDGMGENTA NSSQHTSPEQ
     SVAAQSQHHQ QFIDTAHVFP DFPEPDLGNV LLPEGITMTD IKNLQLMYRR HCEATIDVVM
     NLQFQYIEKL WQAFWNSKPS SPDGSNPMNS EDEQEPIIPK DKLMVLCKYE PIMRWMRNCD
     HILYQALVEI LIPDVLRPVP STLTQAIRNF AKSLEGWLTN AMCDFPQQIV HAKVGVVSAF
     AQTLRRYTSL NHLAQAARAV LQNTSQINQM LSDLNRVDFA NVQEQASWVC QCEEGMVQKL
     EQDFKLTLQQ QSSLDQWANW LDNVVTQVLK PHEGSPSFPK AARQFLLKWS FYSSMVIRDL
     TLRSAASFGS FHLIRLLYDE YMFYLVEHRV AQATGETPIA VMGEFSDFAS MSPVLMDKDD
     VSELGSDNDG DPRISGQPPV KRERVDLNHS MQEM