ID   RFX6_AILME              Reviewed;         928 AA.
AC   D2HNW6;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 2.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=DNA-binding protein RFX6;
DE   AltName: Full=Regulatory factor X 6;
GN   Name=RFX6; ORFNames=PANDA_013423;
OS   Ailuropoda melanoleuca (Giant panda).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Caniformia; Ursidae; Ailuropoda.
OX   NCBI_TaxID=9646;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=20010809; DOI=10.1038/nature08696;
RA   Li R., Fan W., Tian G., Zhu H., He L., Cai J., Huang Q., Cai Q., Li B.,
RA   Bai Y., Zhang Z., Zhang Y., Wang W., Li J., Wei F., Li H., Jian M., Li J.,
RA   Zhang Z., Nielsen R., Li D., Gu W., Yang Z., Xuan Z., Ryder O.A.,
RA   Leung F.C., Zhou Y., Cao J., Sun X., Fu Y., Fang X., Guo X., Wang B.,
RA   Hou R., Shen F., Mu B., Ni P., Lin R., Qian W., Wang G., Yu C., Nie W.,
RA   Wang J., Wu Z., Liang H., Min J., Wu Q., Cheng S., Ruan J., Wang M.,
RA   Shi Z., Wen M., Liu B., Ren X., Zheng H., Dong D., Cook K., Shan G.,
RA   Zhang H., Kosiol C., Xie X., Lu Z., Zheng H., Li Y., Steiner C.C.,
RA   Lam T.T., Lin S., Zhang Q., Li G., Tian J., Gong T., Liu H., Zhang D.,
RA   Fang L., Ye C., Zhang J., Hu W., Xu A., Ren Y., Zhang G., Bruford M.W.,
RA   Li Q., Ma L., Guo Y., An N., Hu Y., Zheng Y., Shi Y., Li Z., Liu Q.,
RA   Chen Y., Zhao J., Qu N., Zhao S., Tian F., Wang X., Wang H., Xu L., Liu X.,
RA   Vinar T., Wang Y., Lam T.W., Yiu S.M., Liu S., Zhang H., Li D., Huang Y.,
RA   Wang X., Yang G., Jiang Z., Wang J., Qin N., Li L., Li J., Bolund L.,
RA   Kristiansen K., Wong G.K., Olson M., Zhang X., Li S., Yang H., Wang J.,
RA   Wang J.;
RT   "The sequence and de novo assembly of the giant panda genome.";
RL   Nature 463:311-317(2010).
CC   -!- FUNCTION: Transcription factor required to direct islet cell
CC       differentiation during endocrine pancreas development. Specifically
CC       required for the differentiation of 4 of the 5 islet cell types and for
CC       the production of insulin. Not required for pancreatic PP (polypeptide-
CC       producing) cells differentiation. Acts downstream of NEUROG3 and
CC       regulates the transcription factors involved in beta-cell maturation
CC       and function, thereby restricting the expression of the beta-cell
CC       differentiation and specification genes, and thus the beta-cell fate
CC       choice. Activates transcription by forming a heterodimer with RFX3 and
CC       binding to the X-box in the promoter of target genes. Involved in
CC       glucose-stimulated insulin secretion by promoting insulin and L-type
CC       calcium channel gene transcription. {ECO:0000250|UniProtKB:Q8HWS3}.
CC   -!- SUBUNIT: Interacts with RFX3. {ECO:0000250|UniProtKB:Q8C7R7}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8HWS3}.
CC   -!- SIMILARITY: Belongs to the RFX family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00858}.
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DR   EMBL; GL193101; EFB21427.1; -; Genomic_DNA.
DR   RefSeq; XP_002924145.1; XM_002924099.1.
DR   AlphaFoldDB; D2HNW6; -.
DR   SMR; D2HNW6; -.
DR   STRING; 9646.ENSAMEP00000006158; -.
DR   PRIDE; D2HNW6; -.
DR   Ensembl; ENSAMET00000006412; ENSAMEP00000006158; ENSAMEG00000005843.
DR   GeneID; 100476328; -.
DR   KEGG; aml:100476328; -.
DR   CTD; 222546; -.
DR   eggNOG; KOG3712; Eukaryota.
DR   GeneTree; ENSGT01050000244879; -.
DR   HOGENOM; CLU_013981_0_0_1; -.
DR   InParanoid; D2HNW6; -.
DR   OMA; GSAPCMF; -.
DR   OrthoDB; 346630at2759; -.
DR   TreeFam; TF321340; -.
DR   Proteomes; UP000008912; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:Ensembl.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IEA:Ensembl.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR   GO; GO:0031018; P:endocrine pancreas development; ISS:UniProtKB.
DR   GO; GO:0042593; P:glucose homeostasis; ISS:UniProtKB.
DR   GO; GO:0003310; P:pancreatic A cell differentiation; ISS:UniProtKB.
DR   GO; GO:0003311; P:pancreatic D cell differentiation; ISS:UniProtKB.
DR   GO; GO:0090104; P:pancreatic epsilon cell differentiation; ISS:UniProtKB.
DR   GO; GO:0035774; P:positive regulation of insulin secretion involved in cellular response to glucose stimulus; ISS:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0050796; P:regulation of insulin secretion; ISS:UniProtKB.
DR   GO; GO:0003309; P:type B pancreatic cell differentiation; ISS:UniProtKB.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR003150; DNA-bd_RFX.
DR   InterPro; IPR039779; RFX-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR12619; PTHR12619; 1.
DR   Pfam; PF02257; RFX_DNA_binding; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS51526; RFX_DBD; 1.
PE   3: Inferred from homology;
KW   Developmental protein; Differentiation; DNA-binding; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..928
FT                   /note="DNA-binding protein RFX6"
FT                   /id="PRO_0000392997"
FT   DNA_BIND        124..199
FT                   /note="RFX-type winged-helix"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00858"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          53..102
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        82..102
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   928 AA;  102414 MW;  A41888418DBCBDCE CRC64;
     MAKVPVLEDA FLPAQPSPQV SPEAQEECCV QLLGKGLLLY PEERVYLAAE AQPGGARGSA
     ERGEHPELPV GVKSEMHLSN GNFSSEEEDG ESHDSKTKAA DLHLSQKKTI TQMMKDKKKQ
     TQLTLQWLEE NYIVCEGVCL PRCILYAHYL DFCRKEKLEP ACAATFGKTI RQKFPLLTTR
     RLGTRGHSKY HYYGIGIKES SAYYHSVYSG KGLTRFSGSK LKNEGGFTRK YSLSSKTGTL
     LPEFPSAQHL VYQGCISKDK VDTLIMMYKT HCQCILDNAI NGNFEEIQHF LLHFWQGMPD
     HLLPLLENPV IIDIFCVCDS ILYKVLTDVL IPATMQEMPE SLLADIRNFA KNWEQWVVSS
     LENLPEALTD KKIPIVRRFV SSLKRQTSFL HLAQIARPAL FDQHVVNSMV SDIEKVDLNS
     IGSQALLTIS GSTDTESDIY TEHDSITVFQ ELKDLLKKNA TVEAFIEWLD TVVEQRVIKT
     SKQNGRSLKK RAQDFLLKWS FFGARVMHNL TLNNASSFGS FHLIRMLLDE YILLAMETQF
     NNDKEQELQN LLDKYMKNSD ASKAAFTASP SSCFLANRNK GSTASSDTVK NESHVETAYL
     PLSSSHPGGF PSALHPFPAG NTDTMPLTGQ MELSQSTGHL MTPPISPAMA SRGSVINQGP
     MAGRPPSVGP VLSAPSHCST FPESIYPTHP QTNQDFYGTN SNYQTVFRAQ PHPPSGLYPH
     RPEHGRCMAW SEQQLSRDFF SGSCAGSPYN SRPPSSYGPS SHSQDSHSMQ FLNTGSFNFL
     SSTGAASCQG ATLPPSSPNG YYGSSINYPE SHRLGSMVNQ HVSVISSVRS LPPYSDIHDP
     LNILDDSSRK QTSSFYADTS PSVACRTPVV ASSLQTSIPS SSSQCMYGTS NQYPAQETLD
     SHGANSREMV SSLPPINTVF MGTAAGGT