ID   RFX6_DANRE              Reviewed;         848 AA.
AC   Q5RJA1;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 2.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=DNA-binding protein RFX6;
DE   AltName: Full=Regulatory factor X 6;
GN   Name=rfx6; ORFNames=si:dkeyp-93d12.2;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX   PubMed=20040487; DOI=10.1242/dev.041673;
RA   Soyer J., Flasse L., Raffelsberger W., Beucher A., Orvain C., Peers B.,
RA   Ravassard P., Vermot J., Voz M.L., Mellitzer G., Gradwohl G.;
RT   "Rfx6 is an Ngn3-dependent winged helix transcription factor required for
RT   pancreatic islet cell development.";
RL   Development 137:203-212(2010).
CC   -!- FUNCTION: Transcription factor required to direct islet cell
CC       differentiation during endocrine pancreas development.
CC       {ECO:0000269|PubMed:20040487}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8HWS3}.
CC   -!- TISSUE SPECIFICITY: Expressed in progenitors and hormone expressing
CC       cells of the islet lineage. {ECO:0000269|PubMed:20040487}.
CC   -!- DISRUPTION PHENOTYPE: Impaired endocrine cell differentiation and
CC       accumulation of islet progenitor cells. {ECO:0000269|PubMed:20040487}.
CC   -!- SIMILARITY: Belongs to the RFX family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00858}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAI21371.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BX000362; CAI21371.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q5RJA1; -.
DR   SMR; Q5RJA1; -.
DR   STRING; 7955.ENSDARP00000116051; -.
DR   PaxDb; Q5RJA1; -.
DR   Ensembl; ENSDART00000061122; ENSDARP00000061121; ENSDARG00000041702.
DR   ZFIN; ZDB-GENE-041014-299; rfx6.
DR   eggNOG; KOG3712; Eukaryota.
DR   GeneTree; ENSGT01050000244879; -.
DR   HOGENOM; CLU_013981_0_0_1; -.
DR   InParanoid; Q5RJA1; -.
DR   PhylomeDB; Q5RJA1; -.
DR   TreeFam; TF321340; -.
DR   PRO; PR:Q5RJA1; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 20.
DR   Bgee; ENSDARG00000041702; Expressed in pancreatic system and 8 other tissues.
DR   ExpressionAtlas; Q5RJA1; baseline.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR   GO; GO:0031018; P:endocrine pancreas development; IMP:UniProtKB.
DR   GO; GO:0042593; P:glucose homeostasis; ISS:UniProtKB.
DR   GO; GO:0003310; P:pancreatic A cell differentiation; ISS:UniProtKB.
DR   GO; GO:0003311; P:pancreatic D cell differentiation; ISS:UniProtKB.
DR   GO; GO:0090104; P:pancreatic epsilon cell differentiation; ISS:UniProtKB.
DR   GO; GO:0035774; P:positive regulation of insulin secretion involved in cellular response to glucose stimulus; ISS:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0050796; P:regulation of insulin secretion; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0003309; P:type B pancreatic cell differentiation; ISS:UniProtKB.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR003150; DNA-bd_RFX.
DR   InterPro; IPR039779; RFX-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR12619; PTHR12619; 1.
DR   Pfam; PF02257; RFX_DNA_binding; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS51526; RFX_DBD; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Differentiation; DNA-binding; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..848
FT                   /note="DNA-binding protein RFX6"
FT                   /id="PRO_0000392998"
FT   DNA_BIND        56..131
FT                   /note="RFX-type winged-helix"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00858"
SQ   SEQUENCE   848 AA;  93762 MW;  B5E0635684AC29C8 CRC64;
     MYVIHLAEAD ESNASISSEE DLGHLDYSKP FAAKQTQPKK SISQIIKDKK KQTQLTLQWL
     EDNYIVCEGV CLPRCILYAH YLDFCRKEKL DPACAATFGK TIRQKFPLLT TRRLGTRGHS
     KYHYYGIGIK ESSAYYHSVY SGKGLTRFSG SKLKNEGGFT RKYSLSSKTG TLLPEFPSAQ
     HLVLQDSVSK EKVDTLIMMY KTHCQCILDN AINVNFEEIQ NFLLHFWQGM PEHLLPLLEN
     PVIVDIFCVC DSILYKVLTD VLIPATMQDM PESLLADIRN FAKHWEHWML SSLENLPEIL
     SEKKVPIARR FVSSLKRQTS FLHLAQIARP ALFDQIVVTS MVNDIDKVDL NSIGSQALLS
     VTNDQDSDFY SEYDSISVFQ ELKDLLRKNA TVESFIEWLD SVVEQKVIKP SKQNGRSVKK
     RAQDFLLKWS FFGARVMHNL TLNNATSFGS FHLIRMLLDE YILLAIETQF NNDKEQDLQN
     LLEKYMRSAD ASKATFTASP SSCFLANRNK PGVSSSDSAV KDEIPPEDDY LTLAAAQQGL
     GAGTVVYHSA DPDNFTIAGQ MDFSQNSAPL MTPPISPAMM NRSSVINQGP MAMRPQTSCP
     IQGQLPCQTF PEPLYQSLHN PPSGSYPNAS YYQPMFRTQT HTESGRYAFS GHHLSKDCFS
     NSCTASPYGT RGSSGGYTAA GDAGMETEAV QMLESSGFGF GGTTGAADGC SGPVCSSGHR
     YYGSSSGYVD APRMGTFIDQ HVSVISSVSS IRSVSAYAEA HDPLNILDDT SRKTRPYYTE
     LSAMGTHTAG SSIAHSCSVS APCMYGAPAP YHSQNTLLPL QGATEIREMM SSLPPINTVF
     MGSTSGPP