RG1BA_DANRE
ID RG1BA_DANRE Reviewed; 514 AA.
AC Q6DHR3; A2AVJ1; Q7SXB0;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Ras-GEF domain-containing family member 1B-A;
GN Name=rasgef1ba; Synonyms=rasgef1b; ORFNames=si:dkey-18e17.1;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=AB;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=17239665; DOI=10.1016/j.modgep.2006.11.010;
RA Epting D., Vorwerk S., Hageman A., Meyer D.;
RT "Expression of rasgef1b in zebrafish.";
RL Gene Expr. Patterns 7:389-395(2007).
CC -!- FUNCTION: Guanine nucleotide exchange factor (GEF) for Ras family
CC proteins (in vitro). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6DHR3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6DHR3-2; Sequence=VSP_034044;
CC -!- TISSUE SPECIFICITY: Detected in oocytes, and in embryos at 4 to 120
CC hours post-fertilization (hpf). Detected along marginal blastomeres at
CC early epiboly stage and throughout the margin at the onset of
CC gastrulation. At 60% epiboly, strongest expression is found in the
CC dorsal shield region and is restricted to the epiblast. Detected in the
CC anterior border of the presomitic mesoderm at the end of epiboly.
CC Detected in adaxial cells, in the somites and in the nervous system
CC during somitogenesis. Detected in diencephalon and hindbrain and in
CC cells surrounding the notochord, including adaxial cells and ventral
CC mesendoderm, in 15-somite stage embryos. At 48 hpf, detected mainly in
CC the brain. {ECO:0000269|PubMed:17239665}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC {ECO:0000269|PubMed:17239665}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH55678.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL929533; CAM15192.1; -; Genomic_DNA.
DR EMBL; AL929533; CAM15193.1; -; Genomic_DNA.
DR EMBL; BC055678; AAH55678.1; ALT_INIT; mRNA.
DR EMBL; BC075904; AAH75904.1; -; mRNA.
DR RefSeq; NP_956123.1; NM_199829.1.
DR RefSeq; XP_005165336.1; XM_005165279.3. [Q6DHR3-1]
DR RefSeq; XP_005165337.1; XM_005165280.3. [Q6DHR3-2]
DR AlphaFoldDB; Q6DHR3; -.
DR SMR; Q6DHR3; -.
DR STRING; 7955.ENSDARP00000116417; -.
DR PaxDb; Q6DHR3; -.
DR PRIDE; Q6DHR3; -.
DR Ensembl; ENSDART00000127123; ENSDARP00000110368; ENSDARG00000033614. [Q6DHR3-2]
DR Ensembl; ENSDART00000137801; ENSDARP00000116417; ENSDARG00000033614. [Q6DHR3-1]
DR Ensembl; ENSDART00000189918; ENSDARP00000151188; ENSDARG00000116057. [Q6DHR3-1]
DR GeneID; 327572; -.
DR KEGG; dre:327572; -.
DR CTD; 327572; -.
DR ZFIN; ZDB-GENE-030131-5783; rasgef1ba.
DR eggNOG; KOG3541; Eukaryota.
DR GeneTree; ENSGT00940000155816; -.
DR HOGENOM; CLU_022907_2_0_1; -.
DR InParanoid; Q6DHR3; -.
DR OMA; THEYYPD; -.
DR OrthoDB; 533115at2759; -.
DR PhylomeDB; Q6DHR3; -.
DR TreeFam; TF313379; -.
DR PRO; PR:Q6DHR3; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 5.
DR Bgee; ENSDARG00000033614; Expressed in somite and 27 other tissues.
DR ExpressionAtlas; Q6DHR3; baseline.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS00720; RASGEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Guanine-nucleotide releasing factor;
KW Reference proteome.
FT CHAIN 1..514
FT /note="Ras-GEF domain-containing family member 1B-A"
FT /id="PRO_0000297641"
FT DOMAIN 76..206
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 246..494
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT VAR_SEQ 1..41
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_034044"
FT CONFLICT 445
FT /note="E -> G (in Ref. 2; AAH55678)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 514 AA; 59890 MW; D1370C294E240B8B CRC64;
MAKFLAELLG CTLPEKGASP MFECRSQPHI GLRHVTKQRD KMPQTPPFPV MYGSTNGYNK
NLYKAKEEDY EGLYYHDNNL ISGSLEALIE HLVPTVAYYP DRTYIFTFLL SSRLFLHPYE
LMSKVCHLCV DQQRLSDPQA DKARVRKIAP KILQLLTEWT ETFPYDFRDE RMMKSLKELT
HRLSTGEDLY RKAVQQMTQT LIRKLTTLSQ YEEALAKINS SVTDRLTVLK TKPQSIQRDI
LTICNDPFTL AQQLTHIELE RLSYIGPEEF VQAFVQKDPL DNDKNCFSDH KKASNLEAYV
EWFNRLSYLV ATEICMPVKK KHRARVIEFF IDVARECFNI GNFNSLMAII TGMNMSPVSR
LKKTWAKVKT AKFDILEHQM DPSSNFYNYR TALRGATQRS ITAHSSREKI VIPFFSLLIK
DIYFLNEGCA NRLPNGHVNF EKFWELAKQV TEFMTWKKVE CPFDRDRKIL QYLLTAPVFS
EDALYLASYE SEGPENNMEK DRWKSLRSSL LNRT
