RG84C_DROME
ID RG84C_DROME Reviewed; 384 AA.
AC P40809; Q9VI91;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=GTPase-activating protein RacGAP84C;
DE AltName: Full=Protein rotund RacGAP;
GN Name=RacGAP84C; Synonyms=rnRacGAP; ORFNames=CG2595;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Oregon-R; TISSUE=Pupae;
RX PubMed=1406685; DOI=10.1128/mcb.12.11.5111-5122.1992;
RA Agnel M., Roeder L., Vola C., Griffin-Shea R.;
RT "A Drosophila rotund transcript expressed during spermatogenesis and
RT imaginal disc morphogenesis encodes a protein which is similar to human Rac
RT GTPase-activating (racGAP) proteins.";
RL Mol. Cell. Biol. 12:5111-5122(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), AND TISSUE
RP SPECIFICITY.
RC TISSUE=Pupae;
RX PubMed=8654933; DOI=10.1016/0378-1119(95)00747-4;
RA Hoemann C.D., Bergeret E., Guichard A., Griffin-Shea R.;
RT "Alternative splicing of the Drosophila melanogaster rotundRacGAP gene.";
RL Gene 168:135-141(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Berkeley; TISSUE=Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Involved in the morphogenesis of the adult appendages.
CC GTPase-activating protein for p21-Rac. Promotes the exchange of Rac-
CC bound GDP by GTP. {ECO:0000269|PubMed:1406685}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=A, m1.7;
CC IsoId=P40809-1; Sequence=Displayed;
CC Name=2; Synonyms=B, m5.3;
CC IsoId=P40809-2; Sequence=VSP_001821;
CC -!- TISSUE SPECIFICITY: In pupae, expressed in imaginal disks and only in
CC the male gonad. In adults; isoform 1 is the predominant form in the
CC testes and is expressed at a low level in the ovary and somatic
CC tissues, and isoform 2 is very weakly expressed and is detectable
CC solely in the testes. {ECO:0000269|PubMed:1406685,
CC ECO:0000269|PubMed:8654933}.
CC -!- DEVELOPMENTAL STAGE: Expressed during spermatogenesis, in primary
CC spermatocytes and imaginal disk morphogenesis.
CC {ECO:0000269|PubMed:1406685}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-23 is the initiator.
CC {ECO:0000305}.
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DR EMBL; M99702; AAA16480.2; -; mRNA.
DR EMBL; U22539; AAC47075.1; -; Genomic_DNA.
DR EMBL; U22539; AAC47076.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF54034.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13349.1; -; Genomic_DNA.
DR EMBL; AY075179; AAL68049.1; -; mRNA.
DR PIR; A48122; A48122.
DR PIR; B48122; B48122.
DR RefSeq; NP_476703.1; NM_057355.4. [P40809-2]
DR RefSeq; NP_476704.1; NM_057356.5. [P40809-1]
DR AlphaFoldDB; P40809; -.
DR SMR; P40809; -.
DR BioGRID; 72922; 23.
DR IntAct; P40809; 1.
DR STRING; 7227.FBpp0081099; -.
DR PaxDb; P40809; -.
DR DNASU; 117503; -.
DR EnsemblMetazoa; FBtr0081580; FBpp0081099; FBgn0045843. [P40809-1]
DR EnsemblMetazoa; FBtr0081581; FBpp0081100; FBgn0045843. [P40809-2]
DR GeneID; 117503; -.
DR KEGG; dme:Dmel_CG2595; -.
DR CTD; 117503; -.
DR FlyBase; FBgn0045843; RacGAP84C.
DR VEuPathDB; VectorBase:FBgn0045843; -.
DR eggNOG; KOG3564; Eukaryota.
DR GeneTree; ENSGT00940000154610; -.
DR HOGENOM; CLU_770027_0_0_1; -.
DR InParanoid; P40809; -.
DR OMA; CNTARPM; -.
DR PhylomeDB; P40809; -.
DR BioGRID-ORCS; 117503; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 117503; -.
DR PRO; PR:P40809; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0045843; Expressed in testis and 6 other tissues.
DR Genevisible; P40809; DM.
DR GO; GO:0097149; C:centralspindlin complex; IBA:GO_Central.
DR GO; GO:0032154; C:cleavage furrow; IBA:GO_Central.
DR GO; GO:0030496; C:midbody; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0051233; C:spindle midzone; IBA:GO_Central.
DR GO; GO:0005096; F:GTPase activator activity; IDA:FlyBase.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0031267; F:small GTPase binding; IPI:FlyBase.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0001745; P:compound eye morphogenesis; IMP:FlyBase.
DR GO; GO:0000281; P:mitotic cytokinesis; IBA:GO_Central.
DR GO; GO:0051256; P:mitotic spindle midzone assembly; IBA:GO_Central.
DR GO; GO:0007266; P:Rho protein signal transduction; IDA:FlyBase.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR CDD; cd00029; C1; 1.
DR Gene3D; 1.10.555.10; -; 1.
DR InterPro; IPR046349; C1-like_sf.
DR InterPro; IPR002219; PE/DAG-bd.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR Pfam; PF00130; C1_1; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00109; C1; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR SUPFAM; SSF57889; SSF57889; 1.
DR PROSITE; PS50238; RHOGAP; 1.
DR PROSITE; PS00479; ZF_DAG_PE_1; 1.
DR PROSITE; PS50081; ZF_DAG_PE_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; Differentiation;
KW Guanine-nucleotide releasing factor; Metal-binding; Reference proteome;
KW Spermatogenesis; Zinc; Zinc-finger.
FT CHAIN 1..384
FT /note="GTPase-activating protein RacGAP84C"
FT /id="PRO_0000080966"
FT DOMAIN 147..330
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT ZN_FING 86..136
FT /note="Phorbol-ester/DAG-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00226"
FT VAR_SEQ 378..384
FT /note="NLSSTHL -> RPMVSL (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_001821"
SQ SEQUENCE 384 AA; 44217 MW; D24959B833B7A2A8 CRC64;
MISGSGSRTP SNRLYLSPVR PTMQNKRRLL REYRSYDDLS EHYRMFGSQS LDSLQDRVDM
NPSGCDGLST DGLDFCSQSH SGLLREHNFK IKSYYYNVGN CVHCRKRIRF AMASLRCRAC
PLRCHIGCCR QLTVNCIPQP QIGTKRGCLS DYAPRVAPMV PALIVHCVTE IEARGLQQEG
LYRVSSTREK CKRLRRKLLR GKSTPHLGNK DTHTLCCCVK DFLRQLVHPL IPIYHRRDFE
EATRHEDRLA VEMAVYLAVL ELHQAHRDTL AYLMLHWQKI AESPAVRMTV NNLAVIFAPT
LFGDLDLTLE NVVTWQRVLK VLLLMPAGFW SQFLEVHPLP TSLGSTYDFE DRYNHRHWDS
SSNLGWSSVK TYFRSMVNLS STHL
