ID   RGA2_BRACM              Reviewed;         579 AA.
AC   Q5BN22;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   12-APR-2005, sequence version 1.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=DELLA protein RGA2;
DE   AltName: Full=BrRGA2;
DE   AltName: Full=RGA-like protein 2;
GN   Name=RGA2;
OS   Brassica campestris (Field mustard).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3711;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15734906; DOI=10.1104/pp.104.057646;
RA   Muangprom A., Thomas S.-G., Sun T.-P., Osborn T.C.;
RT   "A novel dwarfing mutation in a green revolution gene from Brassica rapa.";
RL   Plant Physiol. 137:931-938(2005).
CC   -!- FUNCTION: Probable transcriptional regulator that acts as a repressor
CC       of the gibberellin (GA) signaling pathway. Probably acts by
CC       participating in large multiprotein complexes that represses
CC       transcription of GA-inducible genes. Upon GA application, it is
CC       degraded by the proteasome, allowing the GA signaling pathway (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DOMAIN: The DELLA motif is required for its GA-induced degradation.
CC       {ECO:0000250}.
CC   -!- PTM: Phosphorylated. {ECO:0000250}.
CC   -!- PTM: Ubiquitinated. Upon GA application it is ubiquitinated, leading to
CC       its subsequent degradation (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GRAS family. DELLA subfamily. {ECO:0000305}.
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DR   EMBL; AY928550; AAX33298.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5BN22; -.
DR   SMR; Q5BN22; -.
DR   STRING; 3711.Bra017443.1-P; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1290; -; 1.
DR   InterPro; IPR038088; DELLA_N_sf.
DR   InterPro; IPR030006; TF_DELLA.
DR   InterPro; IPR021914; TF_DELLA_N.
DR   InterPro; IPR005202; TF_GRAS.
DR   PANTHER; PTHR31636; PTHR31636; 1.
DR   PANTHER; PTHR31636:SF245; PTHR31636:SF245; 1.
DR   Pfam; PF12041; DELLA; 1.
DR   Pfam; PF03514; GRAS; 1.
DR   PROSITE; PS50985; GRAS; 1.
PE   3: Inferred from homology;
KW   Gibberellin signaling pathway; Nucleus; Phosphoprotein; Repressor;
KW   Transcription; Transcription regulation; Ubl conjugation.
FT   CHAIN           1..579
FT                   /note="DELLA protein RGA2"
FT                   /id="PRO_0000132240"
FT   DOMAIN          205..574
FT                   /note="GRAS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          1..31
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          149..183
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          212..266
FT                   /note="Leucine repeat I (LRI)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          285..350
FT                   /note="VHIID"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          364..396
FT                   /note="Leucine repeat II (LRII)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          408..495
FT                   /note="PFYRE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   REGION          498..574
FT                   /note="SAW"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   MOTIF           45..49
FT                   /note="DELLA motif"
FT   MOTIF           316..320
FT                   /note="VHIID"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   MOTIF           416..420
FT                   /note="LXXLL motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT   COMPBIAS        14..30
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   579 AA;  63293 MW;  D5AE337B93FBD300 CRC64;
     MKRDLHQFQG PPDTRFPNHG TANTGSSSKD KMMMVKEEED GGNMDELLAV LGYKVRSSEM
     AEVALKLEQL ETMMGNVQED GLSNLATDTV HYNPSELYSW LDNMLTEFNP PPPEINNSFL
     AGAGGSDYDL KAIPGNAIYA RSDQFAIDSS SSSNQAGDNS QSTKRLKSCS SPDSLVTGTT
     VTTTTTESTR SVGLAAESTR SMVLVDSQEN GVRLVHALMA CAEAIQNNDL SIAEALVKQI
     GFLAVSQAGA MRKVATYFAE ALARRIYRLS PPQTQIDHSL SDTLQMHFYE TCPYLKFAHF
     TANQAILEAF EGKKRVHVID FSMNQGLQWP ALMQALALRE GGPPVFRLTG IGPPAADNSD
     HLHEVGCKLA QLAEAIHVEF EYRGFVANSL ADLDASMLEL RPSEIEAVAV NSVFELHKLL
     GRTGGIEKVL GVVKQIKPVI FTVVEQESSH NGPVFLDRFT ESLHYYSTLF DSLEGVPSSQ
     DKVMSEVYLG KQICNLVACE GPDRVERHET LSQWANRFGS SGFAPAHLGS NAFKQASMLL
     ALFNGGEGYR VEENNGCLML GWHTRPLITT SAWKLSAAH