ID   RGA2_SOLBU              Reviewed;         970 AA.
AC   Q7XBQ9; Q7XA18; Q7XA41;
DT   26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Disease resistance protein RGA2;
DE   AltName: Full=Blight resistance protein RPI;
DE   AltName: Full=RGA2-blb;
GN   Name=RGA2; Synonyms=177O13.40, CB3A14.5, RB, RPI-BLB1;
OS   Solanum bulbocastanum (Wild potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=147425;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS.
RX   PubMed=12872003; DOI=10.1073/pnas.1533501100;
RA   Song J., Bradeen J.M., Naess S.K., Raasch J.A., Wielgus S.M.,
RA   Haberlach G.T., Liu J., Kuang H., Austin-Phillips S., Buell C.R.,
RA   Helgeson J.P., Jiang J.;
RT   "Gene RB cloned from Solanum bulbocastanum confers broad spectrum
RT   resistance to potato late blight.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:9128-9133(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=14675451; DOI=10.1046/j.1365-313x.2003.01934.x;
RA   van der Vossen E., Sikkema A., Hekkert Bt B.L., Gros J., Stevens P.,
RA   Muskens M., Wouters D., Pereira A., Stiekema W., Allefs S.;
RT   "An ancient R gene from the wild potato species Solanum bulbocastanum
RT   confers broad-spectrum resistance to Phytophthora infestans in cultivated
RT   potato and tomato.";
RL   Plant J. 36:867-882(2003).
CC   -!- FUNCTION: Disease resistance protein. Resistance proteins guard the
CC       plant against pathogens that contain an appropriate avirulence protein
CC       via a direct or indirect interaction with this avirulence protein. That
CC       triggers a defense system which restricts the pathogen growth. Confers
CC       a broad resistance to all known races of P.infestans.
CC   -!- INDUCTION: Constitutively expressed.
CC   -!- BIOTECHNOLOGY: Can be introduced into cultivated potato (S.tuberosum)
CC       or tomato (L.esculentum) to transfer a broad-spectrum late blight
CC       resistance to cultivated Solanaceae from sexually incompatible host
CC       species.
CC   -!- MISCELLANEOUS: Belongs to a four-gene family located at the same locus.
CC       Although the four genes are expressed in the resistant haplotype, only
CC       RGA2 confers the resistance to P.infestans. In the susceptible
CC       haplotype, RGA1 and RGA3 are likely to be pseudogenes created by
CC       deletions and mutations, while RGA2 contains also several
CC       modifications.
CC   -!- SIMILARITY: Belongs to the disease resistance NB-LRR family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAP45164.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=AAP45188.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AY336128; AAP86601.1; -; Genomic_DNA.
DR   EMBL; AY303170; AAP45164.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AY303171; AAP45188.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AY426259; AAR29069.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q7XBQ9; -.
DR   SMR; Q7XBQ9; -.
DR   PRIDE; Q7XBQ9; -.
DR   GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0098542; P:defense response to other organism; IEA:UniProt.
DR   CDD; cd14798; RX-CC_like; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 1.10.8.430; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.80.10.10; -; 2.
DR   InterPro; IPR042197; Apaf_helical.
DR   InterPro; IPR044974; Disease_R_plants.
DR   InterPro; IPR001611; Leu-rich_rpt.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   InterPro; IPR002182; NB-ARC.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038005; RX-like_CC.
DR   InterPro; IPR041118; Rx_N.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR23155; PTHR23155; 1.
DR   Pfam; PF13855; LRR_8; 1.
DR   Pfam; PF00931; NB-ARC; 1.
DR   Pfam; PF18052; Rx_N; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Leucine-rich repeat; Nucleotide-binding; Plant defense;
KW   Repeat.
FT   CHAIN           1..970
FT                   /note="Disease resistance protein RGA2"
FT                   /id="PRO_0000212778"
FT   DOMAIN          135..438
FT                   /note="NB-ARC"
FT   REPEAT          525..548
FT                   /note="LRR 1"
FT   REPEAT          550..571
FT                   /note="LRR 2"
FT   REPEAT          573..594
FT                   /note="LRR 3"
FT   REPEAT          595..619
FT                   /note="LRR 4"
FT   REPEAT          638..662
FT                   /note="LRR 5"
FT   REPEAT          672..697
FT                   /note="LRR 6"
FT   REPEAT          752..777
FT                   /note="LRR 7"
FT   REPEAT          787..811
FT                   /note="LRR 8"
FT   REPEAT          813..832
FT                   /note="LRR 9"
FT   REPEAT          833..857
FT                   /note="LRR 10"
FT   REPEAT          859..882
FT                   /note="LRR 11"
FT   REPEAT          884..906
FT                   /note="LRR 12"
FT   REPEAT          907..931
FT                   /note="LRR 13"
FT   REPEAT          946..970
FT                   /note="LRR 14"
FT   BINDING         182..189
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   VARIANT         22
FT                   /note="V -> A (in susceptible haplotype)"
FT   VARIANT         449..540
FT                   /note="Missing (in susceptible haplotype)"
FT   VARIANT         889..894
FT                   /note="Missing (in susceptible haplotype)"
SQ   SEQUENCE   970 AA;  110347 MW;  3727029C3CAD9C47 CRC64;
     MAEAFIQVLL DNLTSFLKGE LVLLFGFQDE FQRLSSMFST IQAVLEDAQE KQLNNKPLEN
     WLQKLNAATY EVDDILDEYK TKATRFSQSE YGRYHPKVIP FRHKVGKRMD QVMKKLKAIA
     EERKNFHLHE KIVERQAVRR ETGSVLTEPQ VYGRDKEKDE IVKILINNVS DAQHLSVLPI
     LGMGGLGKTT LAQMVFNDQR VTEHFHSKIW ICVSEDFDEK RLIKAIVESI EGRPLLGEMD
     LAPLQKKLQE LLNGKRYLLV LDDVWNEDQQ KWANLRAVLK VGASGASVLT TTRLEKVGSI
     MGTLQPYELS NLSQEDCWLL FMQRAFGHQE EINPNLVAIG KEIVKKSGGV PLAAKTLGGI
     LCFKREERAW EHVRDSPIWN LPQDESSILP ALRLSYHQLP LDLKQCFAYC AVFPKDAKME
     KEKLISLWMA HGFLLSKGNM ELEDVGDEVW KELYLRSFFQ EIEVKDGKTY FKMHDLIHDL
     ATSLFSANTS SSNIREINKH SYTHMMSIGF AEVVFFYTLP PLEKFISLRV LNLGDSTFNK
     LPSSIGDLVH LRYLNLYGSG MRSLPKQLCK LQNLQTLDLQ YCTKLCCLPK ETSKLGSLRN
     LLLDGSQSLT CMPPRIGSLT CLKTLGQFVV GRKKGYQLGE LGNLNLYGSI KISHLERVKN
     DKDAKEANLS AKGNLHSLSM SWNNFGPHIY ESEEVKVLEA LKPHSNLTSL KIYGFRGIHL
     PEWMNHSVLK NIVSILISNF RNCSCLPPFG DLPCLESLEL HWGSADVEYV EEVDIDVHSG
     FPTRIRFPSL RKLDIWDFGS LKGLLKKEGE EQFPVLEEMI IHECPFLTLS SNLRALTSLR
     ICYNKVATSF PEEMFKNLAN LKYLTISRCN NLKELPTSLA SLNALKSLKI QLCCALESLP
     EEGLEGLSSL TELFVEHCNM LKCLPEGLQH LTTLTSLKIR GCPQLIKRCE KGIGEDWHKI
     SHIPNVNIYI