RGA4R_ORYSJ
ID RGA4R_ORYSJ Reviewed; 996 AA.
AC F7J0M4; F7J0M2; F7J0M3;
DT 18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 1.
DT 25-MAY-2022, entry version 42.
DE RecName: Full=Disease resistance protein RGA4 {ECO:0000305};
DE AltName: Full=Os11gRGA4 {ECO:0000312|EMBL:BAK39922.1};
DE AltName: Full=SasRGA4 {ECO:0000303|PubMed:21251109};
GN Name=RGA4 {ECO:0000303|PubMed:21251109};
GN Synonyms=PIA {ECO:0000303|PubMed:21251109};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP MUTAGENESIS OF CYS-349.
RC STRAIN=cv. Aichi asahi {ECO:0000312|EMBL:BAK39920.1,
RC ECO:0000312|EMBL:BAK39921.1, ECO:0000312|EMBL:BAK39922.1}, and
RC cv. Sasanishiki {ECO:0000312|EMBL:BAK39920.1, ECO:0000312|EMBL:BAK39921.1,
RC ECO:0000312|EMBL:BAK39922.1};
RC TISSUE=Leaf {ECO:0000312|EMBL:BAK39920.1, ECO:0000312|EMBL:BAK39921.1,
RC ECO:0000312|EMBL:BAK39922.1};
RX PubMed=21251109; DOI=10.1111/j.1365-313x.2011.04502.x;
RA Okuyama Y., Kanzaki H., Abe A., Yoshida K., Tamiru M., Saitoh H.,
RA Fujibe T., Matsumura H., Shenton M., Galam D.C., Undan J., Ito A., Sone T.,
RA Terauchi R.;
RT "A multifaceted genomics approach allows the isolation of the rice Pia-
RT blast resistance gene consisting of two adjacent NBS-LRR protein genes.";
RL Plant J. 66:467-479(2011).
RN [2]
RP FUNCTION, AND MUTAGENESIS OF CYS-349.
RC STRAIN=cv. Sasanishiki;
RX PubMed=23548743; DOI=10.1105/tpc.112.107201;
RA Cesari S., Thilliez G., Ribot C., Chalvon V., Michel C., Jauneau A.,
RA Rivas S., Alaux L., Kanzaki H., Okuyama Y., Morel J.B., Fournier E.,
RA Tharreau D., Terauchi R., Kroj T.;
RT "The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe
RT oryzae effectors AVR-Pia and AVR1-CO39 by direct binding.";
RL Plant Cell 25:1463-1481(2013).
RN [3]
RP INTERACTION WITH RGA5, SUBUNIT, FUNCTION, MUTAGENESIS OF LYS-209 AND
RP GLY-502, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Sasanishiki;
RX PubMed=25024433; DOI=10.15252/embj.201487923;
RA Cesari S., Kanzaki H., Fujiwara T., Bernoux M., Chalvon V., Kawano Y.,
RA Shimamoto K., Dodds P., Terauchi R., Kroj T.;
RT "The NB-LRR proteins RGA4 and RGA5 interact functionally and physically to
RT confer disease resistance.";
RL EMBO J. 33:1941-1959(2014).
RN [4]
RP REVIEW.
RX PubMed=25506347; DOI=10.3389/fpls.2014.00606;
RA Cesari S., Bernoux M., Moncuquet P., Kroj T., Dodds P.N.;
RT "A novel conserved mechanism for plant NLR protein pairs: the 'integrated
RT decoy' hypothesis.";
RL Front. Plant Sci. 5:606-606(2014).
RN [5]
RP FUNCTION.
RC STRAIN=cv. Kitaake;
RX PubMed=27289079; DOI=10.1111/tpj.13231;
RA Hutin M., Cesari S., Chalvon V., Michel C., Tran T.T., Boch J., Koebnik R.,
RA Szurek B., Kroj T.;
RT "Ectopic activation of the rice NLR heteropair RGA4/RGA5 confers resistance
RT to bacterial blight and bacterial leaf streak diseases.";
RL Plant J. 88:43-55(2016).
CC -!- FUNCTION: Disease resistance (R) protein. Resistance proteins guard the
CC plant against pathogens that contain an appropriate avirulence protein
CC via an indirect interaction with this avirulence protein. That triggers
CC a defense system including the hypersensitive response, which restricts
CC the pathogen growth. Contribution of RGA5 is required to recognize the
CC effector avirulence proteins AVR-Pia and AVR1-CO39 from M.oryzae
CC (PubMed:21251109, PubMed:23548743). Acts as a constitutively active
CC cell death inducer that is repressed by RGA5 (PubMed:25024433). Immune
CC response triggered by the RGA4-RGA5 -mediated recognition of AVR1-CO39
CC confers resistance to X.oryzae pathovars (PubMed:27289079).
CC {ECO:0000269|PubMed:21251109, ECO:0000269|PubMed:23548743,
CC ECO:0000269|PubMed:25024433, ECO:0000269|PubMed:27289079}.
CC -!- SUBUNIT: Forms homodimer or heterodimer with RGA5 through its coiled
CC coil (CC) domain. {ECO:0000269|PubMed:25024433}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:25024433}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves. {ECO:0000269|PubMed:21251109}.
CC -!- SIMILARITY: Belongs to the disease resistance NB-LRR family.
CC {ECO:0000305}.
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DR EMBL; AB604621; BAK39920.1; -; Genomic_DNA.
DR EMBL; AB604621; BAK39921.1; -; Genomic_DNA.
DR EMBL; AB604622; BAK39922.1; -; mRNA.
DR AlphaFoldDB; F7J0M4; -.
DR SMR; F7J0M4; -.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0043621; F:protein self-association; IPI:UniProtKB.
DR GO; GO:0042742; P:defense response to bacterium; IDA:UniProtKB.
DR GO; GO:0002758; P:innate immune response-activating signal transduction; IDA:UniProtKB.
DR GO; GO:0009626; P:plant-type hypersensitive response; IMP:UniProtKB.
DR GO; GO:0010942; P:positive regulation of cell death; IMP:UniProtKB.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR041118; Rx_N.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR23155; PTHR23155; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF18052; Rx_N; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Coiled coil; Cytoplasm; Leucine-rich repeat;
KW Nucleotide-binding; Plant defense; Repeat.
FT CHAIN 1..996
FT /note="Disease resistance protein RGA4"
FT /id="PRO_0000444659"
FT DOMAIN 180..462
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 481..503
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 504..528
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 529..549
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 577..599
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 600..621
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 622..644
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 698..722
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 759..781
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 782..804
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 805..830
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT REPEAT 851..874
FT /note="LRR 11"
FT /evidence="ECO:0000255"
FT REGION 1..176
FT /note="Structured coiled coil (CC) domain"
FT /evidence="ECO:0000303|PubMed:21251109"
FT COILED 111..138
FT /evidence="ECO:0000255"
FT MUTAGEN 209
FT /note="K->R: Abolishes resistance to M.oryzae pathogen."
FT /evidence="ECO:0000269|PubMed:25024433"
FT MUTAGEN 349
FT /note="C->F: Abolishes resistance to M.oryzae pathogen."
FT /evidence="ECO:0000269|PubMed:21251109,
FT ECO:0000269|PubMed:23548743"
FT MUTAGEN 502
FT /note="G->D: Abolishes resistance to M.oryzae pathogen."
FT /evidence="ECO:0000269|PubMed:25024433"
SQ SEQUENCE 996 AA; 111685 MW; 91FD8843AC096B07 CRC64;
MEAALLSGFI KAILPRLFSL VDDKHKLHKG VKGDIDFLIK ELRMIVGAID DDLSLDHPAA
AAVQTLCMED LRELAHGIED CIDGVLYRAA RDQQQSPVRR AVQAPKKLQR NLQLAQQLQR
LKRMAAEANQ RKQRYTAAAP GQHGQVYSSA AAQVDEPWPS CSSASDPRIH EADLVGVDAD
REELLEQLAE RQPEQLKVIA IVGFCGLGKT ALAAEAYNRE TGGGRFERHA WVCAGHRSAR
EVLGELLRRL DADGRSFHGD SDAGQLCVDI RQQLEKNRYF IVIDDIQTED QWKSIKSAFP
TDKDIGSRIV VTTTIQSVAN ACCSANGYLH KMSRLDKNCS KQLLSKKACP ERYSHYKQPD
SAAILKKCDG QPLALVTIGE FLQANGWPTG PNCEDLCNRL HYHLENDKTL ERMWRVLVRN
YTSLPGHALK ACLLYFGMFP SDHPIRRKSL LRRWLAEGFV EPLSSSSNID STAAFNVLMD
RNIIEPINVS NNDKVKTCQT YGMMREFISH MSISQNFVTF FCDDKFVPKY VRRLSLHGDT
VVNGDNFNGI DLSLVRSLAV FGEAGTTVLD FSKYQLLRVL DLEKCDDLKD DHLKEICNLV
LLKYLSLGGN ISKLPKDIAK LKDLEALDVR RSKVKIMPVE VFGLPCLIHL LGKFKLSDKV
KQKTEVQEFL LKGKSNLQTL AGFASNGSEG FLHLMRYMNK LRKLKIWCTS SAGSTDWTDL
REAIQQFILD EKEANIGTRS LSLHFSGCSE DAINSLKEPC YLSSLKLHGN FPQLPQFVTS
LRGLKELCLS STKFTTGLLE ALSNLSYLQY LKLVADELEK FIIKVQGFPR LLRLCIVLQY
PTFPVIEEGA LPFLVTLQLL CKDLHGLSDI QIECFKHLQE VTLHSGVTPA TRQEWVKAAK
EHPNRPKVLL LKSVDTAESE HTDVDSVMEA VKSETTEYSI APEGPEQVNN KMQLDHGLES
SSVLNKQNNF ADQSSSKDQL HYSFNNMGLS DVSCCE
