RGA4_SCHPO
ID RGA4_SCHPO Reviewed; 933 AA.
AC O74360;
DT 23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Probable Rho-type GTPase-activating protein 4;
GN Name=rga4; ORFNames=SPBC28E12.03;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP GENE NAME.
RX PubMed=11737264; DOI=10.1046/j.1365-2443.2001.00485.x;
RA Nakano K., Mutoh T., Mabuchi I.;
RT "Characterization of GTPase-activating proteins for the function of the
RT Rho-family small GTPases in the fission yeast Schizosaccharomyces pombe.";
RL Genes Cells 6:1031-1042(2001).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353; SER-625; SER-738 AND
RP SER-740, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: GTPase-activating protein for Rho-type proteins.
CC {ECO:0000305}.
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DR EMBL; CU329671; CAA20650.2; -; Genomic_DNA.
DR PIR; T40040; T40040.
DR RefSeq; NP_595756.1; NM_001021656.2.
DR AlphaFoldDB; O74360; -.
DR SMR; O74360; -.
DR BioGRID; 277119; 19.
DR STRING; 4896.SPBC28E12.03.1; -.
DR iPTMnet; O74360; -.
DR MaxQB; O74360; -.
DR PaxDb; O74360; -.
DR PRIDE; O74360; -.
DR EnsemblFungi; SPBC28E12.03.1; SPBC28E12.03.1:pep; SPBC28E12.03.
DR GeneID; 2540593; -.
DR KEGG; spo:SPBC28E12.03; -.
DR PomBase; SPBC28E12.03; rga4.
DR VEuPathDB; FungiDB:SPBC28E12.03; -.
DR eggNOG; KOG1453; Eukaryota.
DR eggNOG; KOG1704; Eukaryota.
DR HOGENOM; CLU_309526_0_0_1; -.
DR InParanoid; O74360; -.
DR PhylomeDB; O74360; -.
DR PRO; PR:O74360; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0071944; C:cell periphery; IDA:PomBase.
DR GO; GO:0005737; C:cytoplasm; IDA:PomBase.
DR GO; GO:0097575; C:lateral cell cortex; IDA:PomBase.
DR GO; GO:0016328; C:lateral plasma membrane; EXP:PomBase.
DR GO; GO:0031097; C:medial cortex; IDA:PomBase.
DR GO; GO:0005096; F:GTPase activator activity; IDA:PomBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0031267; F:small GTPase binding; IPI:PomBase.
DR GO; GO:1903138; P:negative regulation of cell wall integrity MAPK cascade; IMP:PomBase.
DR GO; GO:0061173; P:positive regulation of establishment of bipolar cell polarity; IMP:PomBase.
DR GO; GO:2000784; P:positive regulation of establishment of cell polarity regulating cell shape; EXP:PomBase.
DR GO; GO:0032231; P:regulation of actin filament bundle assembly; IMP:PomBase.
DR GO; GO:0060305; P:regulation of cell diameter; IMP:PomBase.
DR GO; GO:1903338; P:regulation of cell wall organization or biogenesis; IMP:PomBase.
DR GO; GO:0043087; P:regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.555.10; -; 1.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00412; LIM; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00132; LIM; 2.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 1.
DR PROSITE; PS50023; LIM_DOMAIN_2; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 1: Evidence at protein level;
KW GTPase activation; LIM domain; Metal-binding; Phosphoprotein;
KW Reference proteome; Repeat; Zinc.
FT CHAIN 1..933
FT /note="Probable Rho-type GTPase-activating protein 4"
FT /id="PRO_0000075902"
FT DOMAIN 22..80
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 81..129
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 753..932
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 181..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 304..338
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 415..435
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 605..628
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 641..660
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 643..660
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 353
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 625
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 738
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 740
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 933 AA; 104487 MW; 2EE75150F62E5586 CRC64;
MNSGTTLHLD RLSLETPSER TCFCIKCWES VPSTSQVWFG GKCWHSDCFK CVNCNKKLDP
SSEDFSQDDQ KQIFCKLCVD ICNGCSTPIC EFNAVSNSQA NHPCCSNCRA FINSNAFGKF
KGQHYCLPCL RKQDEENQKA VMESASGFIP LRNIAQTAEN TSLTGQNQGF QNPHFIASDS
PSSVLSGRMQ NTSSPTNSLR PQLKVQTNGY ETPGDINSAK SYKDIQKDFL LKPKNSFVKT
SPSPLANGPS FRSANASPFD SCDSFHSGSS IPIEPVSSRQ SSVVNNNSVQ QPVAYHAFVQ
SPTENGTLPQ LPKNESVVNP PPLRRSSTMN YKSVSTTTSP SKYGYVSGRI ALSPIHLRGA
LRDVTNKCNL KVPRNRNSLS NLDEYYVNGL ESDETPTKAR FPRYPTVFNK LDDKRLSSEP
NGLKKRLTNS SNYEASPRAK SLNLSQVSLH QACEPEYNRS SLVRASDVFT SNVFDATEES
ANELAIRISE LQAEVGTLLL EATSLASIIE QQTPVSPEAF KQELVADLNY RLDYLRKSFQ
PELSTLLLRR DALSTTVSKL QNAYSTVMEE TAYLNVKNTE LVSLNNQLER ELAYLREQQH
KKRTSSSFGI FNNDKKSNRT ISTPSPRESF SRLQMVASSL GFRPKDNKDK ESGGYNKRNS
KIDLKKSFSK RFHWKRDSPH MSSTPSISEE HLASEEQEDF VPAVGHCKLC GKYSNELRAH
YQDCVSSTID RQYQSKKSES PVWALNPDDF DQNRLTLLRV PTLIVSCINF IESYGMDFEG
LYRKSGATSQ MKKIVALLRN EDTVLDPSED ISAVTSVFKQ YLRNLPNPII TYDQYFPFIT
AANCASFQDK LDGFIMVIKS LPPAHAEILQ LIIRHLARVA AYSHANRMTS KNLAVVFSPT
LIRDPDNSRD VIDMTVKNYS LAFLIDHVHE VFA
