RGAP3_ARATH
ID RGAP3_ARATH Reviewed; 455 AA.
AC Q8GYY5; Q9ZUZ1;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Rho GTPase-activating protein 3;
DE AltName: Full=Rho-type GTPase-activating protein 3;
GN Name=ROPGAP3; OrderedLocusNames=At2g46710; ORFNames=T3A4.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=22984069; DOI=10.1126/science.1222597;
RA Oda Y., Fukuda H.;
RT "Initiation of cell wall pattern by a Rho- and microtubule-driven symmetry
RT breaking.";
RL Science 337:1333-1336(2012).
CC -!- FUNCTION: Acts as a GTPase activator for the Rac-type GTPase by
CC converting it to an inactive GDP-bound state. Involved in secondary
CC wall pattern formation. In association with ROPGEF4, mediates local
CC activation of ARAC10/ROP11 to initiate the distinct pattern of
CC secondary cell walls in xylem cells. {ECO:0000269|PubMed:22984069}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:22984069};
CC Peripheral membrane protein {ECO:0000305|PubMed:22984069}.
CC Note=Localized at plasma membrane in the secondary wall pits.
CC -!- TISSUE SPECIFICITY: Expressed in differentiating xylem cells.
CC {ECO:0000269|PubMed:22984069}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC69928.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005819; AAC69928.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10744.1; -; Genomic_DNA.
DR EMBL; AK117311; BAC41982.1; -; mRNA.
DR EMBL; BT005369; AAO63433.1; -; mRNA.
DR PIR; C84906; C84906.
DR RefSeq; NP_850458.1; NM_180127.2.
DR AlphaFoldDB; Q8GYY5; -.
DR SMR; Q8GYY5; -.
DR BioGRID; 4618; 1.
DR STRING; 3702.AT2G46710.1; -.
DR iPTMnet; Q8GYY5; -.
DR PaxDb; Q8GYY5; -.
DR PRIDE; Q8GYY5; -.
DR ProteomicsDB; 236179; -.
DR EnsemblPlants; AT2G46710.1; AT2G46710.1; AT2G46710.
DR GeneID; 819283; -.
DR Gramene; AT2G46710.1; AT2G46710.1; AT2G46710.
DR KEGG; ath:AT2G46710; -.
DR Araport; AT2G46710; -.
DR TAIR; locus:2062770; AT2G46710.
DR eggNOG; KOG4270; Eukaryota.
DR HOGENOM; CLU_031591_5_1_1; -.
DR InParanoid; Q8GYY5; -.
DR OMA; WNIEKRN; -.
DR OrthoDB; 500145at2759; -.
DR PhylomeDB; Q8GYY5; -.
DR PRO; PR:Q8GYY5; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8GYY5; baseline and differential.
DR Genevisible; Q8GYY5; AT.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0009531; C:secondary cell wall; IDA:TAIR.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0009664; P:plant-type cell wall organization; IMP:TAIR.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.555.10; -; 1.
DR Gene3D; 3.90.810.10; -; 1.
DR InterPro; IPR000095; CRIB_dom.
DR InterPro; IPR036936; CRIB_dom_sf.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR InterPro; IPR044785; RopGAP1-5.
DR PANTHER; PTHR23177; PTHR23177; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00285; PBD; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Developmental protein; GTPase activation; Membrane;
KW Reference proteome.
FT CHAIN 1..455
FT /note="Rho GTPase-activating protein 3"
FT /id="PRO_0000422718"
FT DOMAIN 105..118
FT /note="CRIB"
FT DOMAIN 153..331
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 1..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 342..366
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 432..455
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..44
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 45..68
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 342..357
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 455 AA; 50782 MW; 0C0779D8F6334EC8 CRC64;
MTNFSRSKST GTIGFPEFKP TRPGPDKYEN IHNDDDEYEE GHSTTSTDYY DASTPLSSHA
SRSGNGSGSG QLTVVDLLAA VLRKSLVMSC AMERGEDDVV ASMDIGWPTE VKHVSHVTFD
RFNGFLGLPS ELEPEVPPRA PSASVSVFGV SAKSMQCSYD DRGNSVPTIL LRMQKRLYTE
GGLKAEGIFR INPDNGKEEH VRRQLNCGVV PRGIDVHCLA GLIKAWFREL PTGVLDVLTP
EQVMRCNTEE DCSRLVILLP PVESAILDWA IGLMADVVEH EQFNKMNARN VAMVFAPNMT
QMADPLTALI HAVQVMNFLK TLILMNLKER ENADAKARWL KKQTSDPSEE WESQHSEILS
PEKPNNNNPK FLRVATLCRL EADNEEEFWN IKKRNDHEGV LDTSSGNGNI GPVQRLCKHP
LFQLSKSTKK AFVSNRDEGR KGREAWSSRL SSLPW
