RGAP4_ARATH
ID RGAP4_ARATH Reviewed; 435 AA.
AC Q9CAX8;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Rho GTPase-activating protein 4;
DE AltName: Full=Rho-type GTPase-activating protein 4;
GN Name=ROPGAP4; OrderedLocusNames=At3g11490; ORFNames=F24K9.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Wassilewskija;
RX PubMed=12065837; DOI=10.1126/science.1071505;
RA Baxter-Burrell A., Yang Z., Springer P.S., Bailey-Serres J.;
RT "RopGAP4-dependent Rop GTPase rheostat control of Arabidopsis oxygen
RT deprivation tolerance.";
RL Science 296:2026-2028(2002).
CC -!- FUNCTION: Acts as a GTPase activator for the Rac-type GTPase by
CC converting it to an inactive GDP-bound state. Acts as negative feedback
CC regulator in tolerance to oxygen deprivation which requires ARAC4/ROP2.
CC {ECO:0000269|PubMed:12065837}.
CC -!- INDUCTION: By oxygen deprivation. {ECO:0000269|PubMed:12065837}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plants have increased sensitivity to stresses
CC induced by oxygen deprivation. {ECO:0000269|PubMed:12065837}.
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DR EMBL; AC008153; AAG51449.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75054.1; -; Genomic_DNA.
DR RefSeq; NP_187756.1; NM_111982.2.
DR AlphaFoldDB; Q9CAX8; -.
DR SMR; Q9CAX8; -.
DR STRING; 3702.AT3G11490.1; -.
DR iPTMnet; Q9CAX8; -.
DR PaxDb; Q9CAX8; -.
DR PRIDE; Q9CAX8; -.
DR ProteomicsDB; 236943; -.
DR EnsemblPlants; AT3G11490.1; AT3G11490.1; AT3G11490.
DR GeneID; 820322; -.
DR Gramene; AT3G11490.1; AT3G11490.1; AT3G11490.
DR KEGG; ath:AT3G11490; -.
DR Araport; AT3G11490; -.
DR TAIR; locus:2080762; AT3G11490.
DR eggNOG; KOG4270; Eukaryota.
DR HOGENOM; CLU_031591_1_1_1; -.
DR InParanoid; Q9CAX8; -.
DR OMA; DENGHQT; -.
DR OrthoDB; 747695at2759; -.
DR PhylomeDB; Q9CAX8; -.
DR PRO; PR:Q9CAX8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9CAX8; baseline and differential.
DR Genevisible; Q9CAX8; AT.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0009664; P:plant-type cell wall organization; IMP:TAIR.
DR GO; GO:0034059; P:response to anoxia; IMP:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.555.10; -; 1.
DR Gene3D; 3.90.810.10; -; 1.
DR InterPro; IPR000095; CRIB_dom.
DR InterPro; IPR036936; CRIB_dom_sf.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR InterPro; IPR044785; RopGAP1-5.
DR PANTHER; PTHR23177; PTHR23177; 1.
DR Pfam; PF00786; PBD; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00285; PBD; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR PROSITE; PS50108; CRIB; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 2: Evidence at transcript level;
KW GTPase activation; Reference proteome; Stress response.
FT CHAIN 1..435
FT /note="Rho GTPase-activating protein 4"
FT /id="PRO_0000422719"
FT DOMAIN 93..106
FT /note="CRIB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00057"
FT DOMAIN 138..319
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 1..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 321..343
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..37
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..59
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 435 AA; 48750 MW; F04088E8D99A1E83 CRC64;
MAKVLKSSQS CHFPSPSSSS STSCGGGNDG SNRDPHSPFN ISRREEEEEE EERSEKERER
FELSSALEIL VSAIRRSVIG GCVGEEDLCS MEIGVPTDVR HVAHVTFDRF HGFLGLPVEF
EPEVPRRAPS ASATVFGVST ESMQLSYDTR GNIVPTILLM MQSHLYSRGG LRVEGIFRIN
GENGQEEYIR EELNKGIIPD NIDVHCLASL IKAWFRELPS GVLDSLSPEQ VMESESEDEC
VELVRLLPST EASLLDWAIN LMADVVEMEQ LNKMNARNIA MVFAPNMTQM LDPLTALMYA
VQVMNFLKTL IVKTLKDRKE SRDKLVPASN PSPRDHNGDQ SSSRQLLHLM KANKEETLDN
FEAEMKDKEE SADEEEEECA ESVELVDIKK SSLVNNSSGG FGQKHIGWEE QRTMSKASSI
VGRVNYRVEL FEAWR
