RGAP7_ARATH
ID RGAP7_ARATH Reviewed; 870 AA.
AC Q8RWQ4; B9DFY3; F4K143; Q3E9B9;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Rho GTPase-activating protein 7;
DE AltName: Full=Rho-type GTPase-activating protein 7;
GN Name=ROPGAP7; OrderedLocusNames=At5g19390; ORFNames=F7K24.140;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
CC -!- FUNCTION: Acts as a GTPase activator for the Rac-type GTPase by
CC converting it to an inactive GDP-bound state. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8RWQ4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8RWQ4-2; Sequence=VSP_046575;
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DR EMBL; AF296837; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92695.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92696.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92698.1; -; Genomic_DNA.
DR EMBL; AY091776; AAM10324.1; -; mRNA.
DR EMBL; BT002224; AAN72235.1; -; mRNA.
DR EMBL; AK316947; BAH19650.1; -; mRNA.
DR EMBL; AK317427; BAH20095.1; -; mRNA.
DR RefSeq; NP_001031905.1; NM_001036828.2. [Q8RWQ4-2]
DR RefSeq; NP_197440.2; NM_121944.3. [Q8RWQ4-1]
DR RefSeq; NP_851042.2; NM_180711.2. [Q8RWQ4-1]
DR AlphaFoldDB; Q8RWQ4; -.
DR SMR; Q8RWQ4; -.
DR STRING; 3702.AT5G19390.2; -.
DR iPTMnet; Q8RWQ4; -.
DR PaxDb; Q8RWQ4; -.
DR PRIDE; Q8RWQ4; -.
DR ProteomicsDB; 236910; -. [Q8RWQ4-1]
DR EnsemblPlants; AT5G19390.1; AT5G19390.1; AT5G19390. [Q8RWQ4-1]
DR EnsemblPlants; AT5G19390.2; AT5G19390.2; AT5G19390. [Q8RWQ4-1]
DR EnsemblPlants; AT5G19390.4; AT5G19390.4; AT5G19390. [Q8RWQ4-2]
DR GeneID; 832059; -.
DR Gramene; AT5G19390.1; AT5G19390.1; AT5G19390. [Q8RWQ4-1]
DR Gramene; AT5G19390.2; AT5G19390.2; AT5G19390. [Q8RWQ4-1]
DR Gramene; AT5G19390.4; AT5G19390.4; AT5G19390. [Q8RWQ4-2]
DR KEGG; ath:AT5G19390; -.
DR Araport; AT5G19390; -.
DR TAIR; locus:2150265; AT5G19390.
DR eggNOG; KOG4271; Eukaryota.
DR HOGENOM; CLU_011283_1_0_1; -.
DR InParanoid; Q8RWQ4; -.
DR OMA; NWDFNLR; -.
DR OrthoDB; 209269at2759; -.
DR PhylomeDB; Q8RWQ4; -.
DR PRO; PR:Q8RWQ4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8RWQ4; baseline and differential.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0005096; F:GTPase activator activity; ISS:TAIR.
DR GO; GO:0009920; P:cell plate formation involved in plant-type cell wall biogenesis; IGI:TAIR.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.555.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR025757; MIP1_Leuzipper.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR Pfam; PF14389; Lzipper-MIP1; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; GTPase activation; Reference proteome.
FT CHAIN 1..870
FT /note="Rho GTPase-activating protein 7"
FT /id="PRO_0000422722"
FT DOMAIN 18..125
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 167..367
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 378..432
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 446..465
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 736..793
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 569..693
FT /evidence="ECO:0000255"
FT COMPBIAS 387..401
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 408..422
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 752..793
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 715..870
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_046575"
FT CONFLICT 245
FT /note="V -> I (in Ref. 4; BAH19650)"
FT /evidence="ECO:0000305"
FT CONFLICT 384
FT /note="G -> E (in Ref. 4; BAH19650)"
FT /evidence="ECO:0000305"
FT CONFLICT 399
FT /note="K -> E (in Ref. 4; BAH19650)"
FT /evidence="ECO:0000305"
FT CONFLICT 673
FT /note="Missing (in Ref. 4; BAH19650)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 870 AA; 96128 MW; 31B838F03D1B6933 CRC64;
MEASLAALER PRGSASNTVF KSGPLFISSK GLGWTSWKKR WFILTRTSLV FFKNDPGTLP
QKGGEVNLTL GGIDLNNSGS VVVREDKKLL TVLFPDGRDG RAFTLKAETF EDLYEWKTAL
EQALAQAPNA ALIMGQNGIF RAETNEAIEG REKRPLKSLV VGRPILLALE DIDGSPSFLE
KALQFIEKYG TKIEGILRQS ADVEEVERRV QEYEQGKTEF TFDEDPHVVG DCIKHVLREL
PSSPVSASCC TALLEAYRIE SKEARISSLR SAIAETFPEP NRRLLQRILK MMHTISSHSH
ENRMNPNAVA ACMAPLLLRP LLAGECDLED DFDGGEDNSA QLLAAANAAN NAQAIITVLL
EDYGSIFDEE NIQRCSISTE SHIGNSGPDD SSDDDNNMKN GYHNADNEVE PVTDDDNDRA
LSGKMSESSG CTGSDLYEYK GFVADDSDIE SPRDTNGPRC NSNIRTDHLM RNPFVNSTDQ
QAGEQIGDDP TKYGVNSCLA HVSESYQQSG TGLNVPTHGN TLAAPGLESP SAKSVNKGTP
SSVHAKRATF WGRGSARKIS TDGSFDSSGE DELAIQRLET TKNELRQRIA KEARGNAILQ
ASLERRKQAL HERRLSLEQD VSRLQEQLQA ERDLRAALEV GLSMSSGQFS SHGVDSKTRA
ELEEIALAEA DVARLKQKVA ELHHQLNQQR QTHFGSFSDA RDTHQYLQNH NPQKRFLQQD
FDSTLAYVNH ERKQRHEENV LGAEWRNSKG AGSFGVGNSR QPSRKQIPES TNTTDSKISE
ESGKISVDKL SSIDSPSIPS TSRVLDITEY PRLNHPSAAA SAALVELTTR LDFFKERRSQ
LMEQLQNLDL NYGGSSSQDF IHRPSSPPWN
