ID   RGAPB_DICDI             Reviewed;         418 AA.
AC   Q54SS8;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=RapA guanosine triphosphatase-activating protein B;
GN   Name=rapgapB; ORFNames=DDB_G0282247;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RX   PubMed=19126673; DOI=10.1242/jcs.036822;
RA   Parkinson K., Bolourani P., Traynor D., Aldren N.L., Kay R.R., Weeks G.,
RA   Thompson C.R.;
RT   "Regulation of Rap1 activity is required for differential adhesion, cell-
RT   type patterning and morphogenesis in Dictyostelium.";
RL   J. Cell Sci. 122:335-344(2009).
CC   -!- FUNCTION: Mediates the deactivation of rap1 during multicellular
CC       development and is required for normal morphogenesis. Also required for
CC       the correct patterning of specific subtypes of prestalk cells.
CC       {ECO:0000269|PubMed:19126673}.
CC   -!- DEVELOPMENTAL STAGE: Expressed constantly during multicellular
CC       development and is enriched in prestalk cells.
CC       {ECO:0000269|PubMed:19126673}.
CC   -!- DISRUPTION PHENOTYPE: Null cells have increased levels of active rap1,
CC       increased substrate adhesion, abnormal F-actin distribution, show
CC       defects in cell-cell and substrate adhesion during aggregation and
CC       vegetative growth. {ECO:0000269|PubMed:19126673}.
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DR   EMBL; AAFI02000046; EAL66329.1; -; Genomic_DNA.
DR   RefSeq; XP_640307.1; XM_635215.1.
DR   AlphaFoldDB; Q54SS8; -.
DR   SMR; Q54SS8; -.
DR   STRING; 44689.DDB0233728; -.
DR   PaxDb; Q54SS8; -.
DR   EnsemblProtists; EAL66329; EAL66329; DDB_G0282247.
DR   GeneID; 8623483; -.
DR   KEGG; ddi:DDB_G0282247; -.
DR   dictyBase; DDB_G0282247; rapgapB.
DR   eggNOG; KOG3686; Eukaryota.
DR   HOGENOM; CLU_657925_0_0_1; -.
DR   InParanoid; Q54SS8; -.
DR   OMA; PNCIKSK; -.
DR   PhylomeDB; Q54SS8; -.
DR   Reactome; R-DDI-392517; Rap1 signalling.
DR   PRO; PR:Q54SS8; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005096; F:GTPase activator activity; IMP:dictyBase.
DR   GO; GO:0007015; P:actin filament organization; IMP:dictyBase.
DR   GO; GO:0090630; P:activation of GTPase activity; IMP:dictyBase.
DR   GO; GO:0031589; P:cell-substrate adhesion; IMP:dictyBase.
DR   GO; GO:0022407; P:regulation of cell-cell adhesion; IMP:dictyBase.
DR   GO; GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
DR   GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR   Gene3D; 3.40.50.11210; -; 1.
DR   InterPro; IPR035974; Rap/Ran-GAP_sf.
DR   InterPro; IPR000331; Rap/Ran_GAP_dom.
DR   Pfam; PF02145; Rap_GAP; 1.
DR   SUPFAM; SSF111347; SSF111347; 1.
DR   PROSITE; PS50085; RAPGAP; 1.
PE   2: Evidence at transcript level;
KW   GTPase activation; Reference proteome.
FT   CHAIN           1..418
FT                   /note="RapA guanosine triphosphatase-activating protein B"
FT                   /id="PRO_0000368230"
FT   DOMAIN          142..407
FT                   /note="Rap-GAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00165"
FT   REGION          304..339
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   418 AA;  46889 MW;  802804955E478675 CRC64;
     MFSHCEGYRI EGGVTDGETN WNNNFNQNNN NNDNNKVMAS IYKQQFYGKP HKTFMSLSSP
     NGPILVCIKK VGNSSDDFYL LVCTEKGFEE VRANKDSLQK SKTTRSFLKM RPTSLNVITS
     VRPELTKVPL CKVTDKSIQE ELVKVCEPEF NKVIKSALLY CKESQRDDND MLKNISSNVS
     QEYNDFLNFL GEKVELKDFS KFNGGLDIKN NSHGTHSIYS QINDVEVMYH VATMLPFFPS
     DPKQSERRKL ISLDRVVIIF NDGSKPMSPN CIKSKSTQII ILIQPIKNFV GNSKTTIGIS
     NDENRVVGEQ PSPSLTTTTT TTTTTSPTIN SNSPTPSNKI KYRVSISNRD EVPNYGPPLP
     DPPIFEKDDS FRNFLYQKMV GGAASLRNTP AFTSKNSEKA SALINVISKY STKGMEQI