ID   RGAPX_ARATH             Reviewed;         376 AA.
AC   Q3E875; Q8LEE6; Q94AM7;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 2.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Uncharacterized Rho GTPase-activating protein At5g61530;
GN   OrderedLocusNames=At5g61530; ORFNames=K11J9.10;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA   Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT   features of the regions of 1,367,185 bp covered by 19 physically assigned
RT   P1 and TAC clones.";
RL   DNA Res. 5:203-216(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RX   PubMed=14993207; DOI=10.1101/gr.1515604;
RA   Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA   Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA   Weissenbach J., Salanoubat M.;
RT   "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT   combined approach to evaluate and improve Arabidopsis genome annotation.";
RL   Genome Res. 14:406-413(2004).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Seedling;
RX   PubMed=17586839; DOI=10.1074/mcp.m700164-mcp200;
RA   Niittylae T., Fuglsang A.T., Palmgren M.G., Frommer W.B., Schulze W.X.;
RT   "Temporal analysis of sucrose-induced phosphorylation changes in plasma
RT   membrane proteins of Arabidopsis.";
RL   Mol. Cell. Proteomics 6:1711-1726(2007).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q3E875-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q3E875-2; Sequence=VSP_032379;
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DR   EMBL; AB012239; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002688; AED97480.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97481.1; -; Genomic_DNA.
DR   EMBL; CP002688; ANM70654.1; -; Genomic_DNA.
DR   EMBL; AY045924; AAK76598.1; -; mRNA.
DR   EMBL; AY133882; AAM91816.1; -; mRNA.
DR   EMBL; AY085460; AAM62686.1; -; mRNA.
DR   EMBL; BX831138; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; NP_001332245.1; NM_001345479.1. [Q3E875-2]
DR   RefSeq; NP_568935.1; NM_125546.3. [Q3E875-1]
DR   RefSeq; NP_974973.1; NM_203244.2. [Q3E875-2]
DR   AlphaFoldDB; Q3E875; -.
DR   SMR; Q3E875; -.
DR   BioGRID; 21518; 1.
DR   IntAct; Q3E875; 1.
DR   STRING; 3702.AT5G61530.1; -.
DR   iPTMnet; Q3E875; -.
DR   PaxDb; Q3E875; -.
DR   PRIDE; Q3E875; -.
DR   ProteomicsDB; 236884; -. [Q3E875-1]
DR   EnsemblPlants; AT5G61530.1; AT5G61530.1; AT5G61530. [Q3E875-1]
DR   EnsemblPlants; AT5G61530.2; AT5G61530.2; AT5G61530. [Q3E875-2]
DR   EnsemblPlants; AT5G61530.5; AT5G61530.5; AT5G61530. [Q3E875-2]
DR   GeneID; 836274; -.
DR   Gramene; AT5G61530.1; AT5G61530.1; AT5G61530. [Q3E875-1]
DR   Gramene; AT5G61530.2; AT5G61530.2; AT5G61530. [Q3E875-2]
DR   Gramene; AT5G61530.5; AT5G61530.5; AT5G61530. [Q3E875-2]
DR   KEGG; ath:AT5G61530; -.
DR   Araport; AT5G61530; -.
DR   TAIR; locus:2151611; AT5G61530.
DR   eggNOG; KOG4270; Eukaryota.
DR   HOGENOM; CLU_040482_0_0_1; -.
DR   InParanoid; Q3E875; -.
DR   OMA; MWQQGDS; -.
DR   PhylomeDB; Q3E875; -.
DR   PRO; PR:Q3E875; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q3E875; baseline and differential.
DR   Genevisible; Q3E875; AT.
DR   GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR   GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR   Gene3D; 1.10.555.10; -; 1.
DR   InterPro; IPR008936; Rho_GTPase_activation_prot.
DR   InterPro; IPR000198; RhoGAP_dom.
DR   Pfam; PF00620; RhoGAP; 1.
DR   SMART; SM00324; RhoGAP; 1.
DR   SUPFAM; SSF48350; SSF48350; 1.
DR   PROSITE; PS50238; RHOGAP; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; GTPase activation; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..376
FT                   /note="Uncharacterized Rho GTPase-activating protein
FT                   At5g61530"
FT                   /id="PRO_0000324840"
FT   DOMAIN          139..367
FT                   /note="Rho-GAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT   REGION          307..338
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         59
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17586839"
FT   VAR_SEQ         268..276
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14993207"
FT                   /id="VSP_032379"
SQ   SEQUENCE   376 AA;  41923 MW;  D5933042C83A0B03 CRC64;
     MPSLISQQWQ ERTSGFFSSS GTKLREAGQT AGSFVGEVAK DAKVNVADVA ERVGSLFKSR
     WAILQQPATR HAVQEHLITA AATTGTFVRK GITETKEKVS VGKIKVEEAA KKTAQKSKTI
     LTDIERWQKG VASSDVFGVA IEITVQRQES SRPIPLILVK CADYLILTGL NSPNLFKAEG
     DRKLIQQLVS AYNQDPRASI PEGVNPVDVA ALLKYYLASL PTPLTTFELY NEIKDARSSI
     HRMRQSLQKL SNVNYNTLEF ITALLLRVSQ KSLLNKMDSH SLAMEMAPVI MWREDNRPES
     YREYWRRPSR SPKKSNDFET ATPWDLLSDE GEGPDASSSI PLDDIARVDF GAVEVVQCLI
     EHHNAIFTDA AETVWR