RGA_DROME
ID RGA_DROME Reviewed; 585 AA.
AC Q94547; Q1LZ18; Q8IPQ1; Q8T3L5; Q9VNE5;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Regulator of gene activity;
DE Short=Protein regena;
DE AltName: Full=CCR4-NOT transcription complex subunit 2 homolog;
DE Short=CNOT2;
DE Short=Not2;
GN Name=Rga {ECO:0000312|FlyBase:FBgn0017550};
GN Synonyms=Not2 {ECO:0000303|PubMed:23303381,
GN ECO:0000312|FlyBase:FBgn0017550}; ORFNames=CG2161;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAM11125.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=Canton-S {ECO:0000269|PubMed:9475742};
RC TISSUE=Eye-antennal disk {ECO:0000269|PubMed:9475742};
RX PubMed=9475742; DOI=10.1093/genetics/148.1.317;
RA Frolov M.V., Benevolenskaya E.V., Birchler J.A.;
RT "Regena (Rga), a Drosophila homolog of the global negative transcriptional
RT regulator CDC36 (NOT2) from yeast, modifies gene expression and suppresses
RT position effect variegation.";
RL Genetics 148:317-329(1998).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537569};
RC TISSUE=Ovary {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, AND IDENTIFICATION IN THE CCR4-NOT COMPLEX.
RX PubMed=20504953; DOI=10.1261/rna.2145110;
RA Temme C., Zhang L., Kremmer E., Ihling C., Chartier A., Sinz A.,
RA Simonelig M., Wahle E.;
RT "Subunits of the Drosophila CCR4-NOT complex and their roles in mRNA
RT deadenylation.";
RL RNA 16:1356-1370(2010).
RN [7]
RP FUNCTION, IDENTIFICATION IN THE CCR4-NOT COMPLEX, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=23303381; DOI=10.4161/rna.23018;
RA Bawankar P., Loh B., Wohlbold L., Schmidt S., Izaurralde E.;
RT "NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT complex
RT that docks onto the NOT1 N-terminal domain.";
RL RNA Biol. 10:228-244(2013).
RN [8]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=29887366; DOI=10.1016/j.stemcr.2018.05.005;
RA Ng A.Y.E., Peralta K.R.G., Pek J.W.;
RT "Germline Stem Cell Heterogeneity Supports Homeostasis in Drosophila.";
RL Stem Cell Reports 11:13-21(2018).
CC -!- FUNCTION: Component of the CCR4-NOT complex which is one of the major
CC cellular mRNA deadenylases and is linked to various cellular processes
CC including bulk mRNA degradation, miRNA-mediated repression,
CC translational repression during translational initiation and general
CC transcription regulation (PubMed:20504953, PubMed:23303381). Additional
CC complex functions may be a consequence of its influence on mRNA
CC expression (PubMed:23303381). Essential for viability (PubMed:9475742).
CC Acts as a suppressor of position effect variegation (PEV) at the white
CC locus and regulates the expression of several unrelated genes
CC (PubMed:9475742). Plays a role in germline stem cell differentiation in
CC the ovaries (PubMed:29887366). {ECO:0000269|PubMed:20504953,
CC ECO:0000269|PubMed:23303381, ECO:0000269|PubMed:29887366,
CC ECO:0000269|PubMed:9475742}.
CC -!- SUBUNIT: Component of the CCR4-NOT complex composed of at least
CC Pop2/Caf1-55, Ccr4, Not1, Rga/Not2, and Not3.
CC {ECO:0000269|PubMed:20504953, ECO:0000269|PubMed:23303381}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23303381}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A {ECO:0000303|PubMed:10731132};
CC IsoId=Q94547-1; Sequence=Displayed;
CC Name=B {ECO:0000303|PubMed:10731132};
CC IsoId=Q94547-2; Sequence=VSP_010196;
CC -!- TISSUE SPECIFICITY: Expressed in heterogeneous levels between adjacent
CC germline stem cells (at protein level). {ECO:0000269|PubMed:29887366}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos and adult (at protein level).
CC {ECO:0000269|PubMed:20504953}.
CC -!- SIMILARITY: Belongs to the CNOT2/3/5 family. {ECO:0000305}.
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DR EMBL; U75467; AAB18342.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF51992.2; -; Genomic_DNA.
DR EMBL; AE014297; AAN13250.1; -; Genomic_DNA.
DR EMBL; AY094772; AAM11125.1; -; mRNA.
DR EMBL; BT025208; ABF17899.1; -; mRNA.
DR RefSeq; NP_001287183.1; NM_001300254.1. [Q94547-2]
DR RefSeq; NP_524239.2; NM_079515.3. [Q94547-2]
DR RefSeq; NP_730966.1; NM_169080.3. [Q94547-1]
DR AlphaFoldDB; Q94547; -.
DR SMR; Q94547; -.
DR BioGRID; 65889; 22.
DR IntAct; Q94547; 1.
DR STRING; 7227.FBpp0078390; -.
DR PaxDb; Q94547; -.
DR PRIDE; Q94547; -.
DR DNASU; 40683; -.
DR EnsemblMetazoa; FBtr0078741; FBpp0078390; FBgn0017550. [Q94547-1]
DR EnsemblMetazoa; FBtr0078742; FBpp0078391; FBgn0017550. [Q94547-2]
DR EnsemblMetazoa; FBtr0345140; FBpp0311361; FBgn0017550. [Q94547-2]
DR GeneID; 40683; -.
DR KEGG; dme:Dmel_CG2161; -.
DR UCSC; CG2161-RA; d. melanogaster. [Q94547-1]
DR CTD; 40683; -.
DR FlyBase; FBgn0017550; Rga.
DR VEuPathDB; VectorBase:FBgn0017550; -.
DR eggNOG; KOG2151; Eukaryota.
DR GeneTree; ENSGT00390000001285; -.
DR InParanoid; Q94547; -.
DR OMA; RGVYLLW; -.
DR PhylomeDB; Q94547; -.
DR BioGRID-ORCS; 40683; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40683; -.
DR PRO; PR:Q94547; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0017550; Expressed in cleaving embryo and 45 other tissues.
DR ExpressionAtlas; Q94547; baseline and differential.
DR Genevisible; Q94547; DM.
DR GO; GO:0030014; C:CCR4-NOT complex; IDA:FlyBase.
DR GO; GO:0030015; C:CCR4-NOT core complex; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; NAS:UniProtKB.
DR GO; GO:0000932; C:P-body; IBA:GO_Central.
DR GO; GO:0036099; P:female germ-line stem cell population maintenance; IMP:UniProtKB.
DR GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR GO; GO:0006402; P:mRNA catabolic process; IDA:FlyBase.
DR GO; GO:0017148; P:negative regulation of translation; IDA:FlyBase.
DR GO; GO:0000288; P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; IMP:FlyBase.
DR GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IMP:FlyBase.
DR GO; GO:2000036; P:regulation of stem cell population maintenance; IGI:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR Gene3D; 2.30.30.1020; -; 1.
DR InterPro; IPR038635; CCR4-NOT_su2/3/5_N_sf.
DR InterPro; IPR040168; Not2/3/5.
DR InterPro; IPR007282; NOT2/3/5_C.
DR PANTHER; PTHR23326; PTHR23326; 1.
DR Pfam; PF04153; NOT2_3_5; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Reference proteome;
KW RNA-mediated gene silencing; Transcription; Transcription regulation;
KW Translation regulation.
FT CHAIN 1..585
FT /note="Regulator of gene activity"
FT /id="PRO_0000198335"
FT REGION 42..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 148..191
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 349..394
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 156..191
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 356..394
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 194..200
FT /note="GNFFTSF -> V (in isoform B)"
FT /evidence="ECO:0000303|PubMed:10731132"
FT /id="VSP_010196"
FT CONFLICT 19
FT /note="A -> R (in Ref. 1; AAB18342)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 585 AA; 59973 MW; 12A5EC046C7AD3C5 CRC64;
MANLNFQQPP RSIANAALAG RTTGGFGGSS LAGHVTPTSG MFQTDFANSY PGTANYGQAP
QQQQQQQQQP QLSPNRNAQL SVGGPAISSG NRNANLFGQR QFVERRAMQG LGSGPMSNMG
NFMQTGRGGY GTGGGGGGPL NNFHVFGGGG GSDTSTPALL DPTEFPSLTN ARGQNDQTLP
QSNPLQPPGS KPYGNFFTSF GMVKQPTSEQ SEFTMSNEDF PALPGTQNSD GTTNAVGSVA
GTGGSGGAST ENHLDGTEKP MNSIVVSGSA SGSSGSNVGV VGGNGLGAVG SGIGGLAVGG
GGGAGSSGGG GVGGNAASGV VGGSHVGLVG SNSGIGGVNS VPNSNAMMGV GGGLGSGSGS
SGSGAGGEHL NDNSSNDKLV KSGVQTSPDG KVTNIPATMV NNQFGMVGLL TFIRAAETDP
NLVTLSLGTD LTGLGLNLNS QESLHTTFAG PFVAQPCRAQ DVEFNVPPEY LINFAIRDKL
TAPVLKKLQE DLLFFLFYTN IGDMMQLMAA AELHSREWRY HVEEKIWITR IPGIDQYEKN
GTKERGTFYY FDAQSWKRLS KVFQIDPEKL DKCPNISAFM NGQSV
