RGF10_ARATH
ID RGF10_ARATH Reviewed; 143 AA.
AC F4JBX1; Q9LZZ8;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2017, sequence version 1.
DT 03-AUG-2022, entry version 30.
DE RecName: Full=Root meristem growth factor 10 {ECO:0000303|PubMed:27001831};
DE Short=AtRGF10 {ECO:0000303|PubMed:27001831};
DE AltName: Full=CLAVATA3/ESR (CLE)-related protein CLELn {ECO:0000303|PubMed:22815541};
DE Short=AtCLELn {ECO:0000303|PubMed:22815541};
DE Short=Nuclear CLE-Like protein {ECO:0000303|PubMed:22815541};
DE Contains:
DE RecName: Full=CLELn {ECO:0000303|PubMed:22815541};
DE Flags: Precursor;
GN Name=RGF10 {ECO:0000303|PubMed:27001831};
GN Synonyms=CLELn {ECO:0000303|PubMed:22815541};
GN OrderedLocusNames=At3g60650 {ECO:0000312|Araport:AT3G60650};
GN ORFNames=T4C21.60 {ECO:0000312|EMBL:CAB82669.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION,
RP AND INDUCTION BY AUXIN.
RC STRAIN=cv. Columbia;
RX PubMed=22815541; DOI=10.1093/mp/sss060;
RA Meng L., Buchanan B.B., Feldman L.J., Luan S.;
RT "A putative nuclear CLE-like (CLEL) peptide precursor regulates root growth
RT in Arabidopsis.";
RL Mol. Plant 5:955-957(2012).
RN [4]
RP INTERACTION WITH RGI1; RGI2 AND RGI3.
RC STRAIN=cv. Columbia;
RX PubMed=27001831; DOI=10.1073/pnas.1522639113;
RA Shinohara H., Mori A., Yasue N., Sumida K., Matsubayashi Y.;
RT "Identification of three LRR-RKs involved in perception of root meristem
RT growth factor in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 113:3897-3902(2016).
CC -!- FUNCTION: [CLELn]: Maintains the postembryonic root stem cell niche by
CC regulating the expression levels and patterns of the transcription
CC factor PLETHORA (PLT), mainly at the post-transcriptional level (By
CC similarity). Promotes root elongation (PubMed:22815541).
CC {ECO:0000250|UniProtKB:Q3E880, ECO:0000269|PubMed:22815541}.
CC -!- SUBUNIT: [CLELn]: Binds to LRR receptor-like serine/threonine-protein
CC kinases RGI1, RGI2 and RGI3 to trigger their dimerization with SERK
CC proteins and subsequent signaling. {ECO:0000269|PubMed:27001831}.
CC -!- SUBCELLULAR LOCATION: [CLELn]: Secreted {ECO:0000250|UniProtKB:Q3E880}.
CC Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768,
CC ECO:0000269|PubMed:22815541}.
CC -!- TISSUE SPECIFICITY: Expressed in root tips.
CC {ECO:0000269|PubMed:22815541}.
CC -!- DEVELOPMENTAL STAGE: Expressed specifically in the quiescent center
CC (QC) of the root apical meristem (RAM). {ECO:0000269|PubMed:22815541}.
CC -!- INDUCTION: Induced by auxin. {ECO:0000269|PubMed:22815541}.
CC -!- PTM: The tyrosine sulfation is critical for the function of the
CC peptide. {ECO:0000250|UniProtKB:Q3E880}.
CC -!- MISCELLANEOUS: When applied in the extracellular space, triggers a root
CC waving phenotype (PubMed:22815541). But seems to have a physiological
CC nuclear subcellular location where it promotes root elongation
CC (PubMed:22815541). {ECO:0000269|PubMed:22815541}.
CC -!- SIMILARITY: Belongs to the RGF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB82669.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL162295; CAB82669.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE80093.2; -; Genomic_DNA.
DR PIR; T47876; T47876.
DR RefSeq; NP_001319807.1; NM_001340053.1.
DR AlphaFoldDB; F4JBX1; -.
DR PRIDE; F4JBX1; -.
DR EnsemblPlants; AT3G60650.1; AT3G60650.1; AT3G60650.
DR GeneID; 825236; -.
DR Gramene; AT3G60650.1; AT3G60650.1; AT3G60650.
DR KEGG; ath:AT3G60650; -.
DR Araport; AT3G60650; -.
DR TAIR; locus:2101831; AT3G60650.
DR eggNOG; ENOG502R1ST; Eukaryota.
DR HOGENOM; CLU_2295601_0_0_1; -.
DR OMA; EAPSTNK; -.
DR OrthoDB; 1539447at2759; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4JBX1; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0048527; P:lateral root development; IMP:UniProtKB.
DR GO; GO:2000280; P:regulation of root development; IMP:UniProtKB.
DR GO; GO:2000067; P:regulation of root morphogenesis; IMP:UniProtKB.
DR GO; GO:0009733; P:response to auxin; IEP:UniProtKB.
PE 1: Evidence at protein level;
KW Differentiation; Glycoprotein; Growth factor; Hydroxylation; Nucleus;
KW Reference proteome; Secreted; Signal; Sulfation.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT PROPEP 28..130
FT /evidence="ECO:0000250|UniProtKB:Q3E880"
FT /id="PRO_0000451979"
FT PEPTIDE 131..143
FT /note="CLELn"
FT /evidence="ECO:0000250|UniProtKB:Q3E880"
FT /id="PRO_0000451980"
FT REGION 74..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 92..99
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT MOD_RES 132
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q3E880"
FT MOD_RES 140
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT CARBOHYD 60
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 143 AA; 16484 MW; 7FEA3F17FD78023C CRC64;
MDMLRSACFY FLLIVFVILS WSLLCDSRHL GHMEKKLSVN LDLLNKDNEE ITKLEAPSTN
KTNTLLSQSH AVVNHGDNGQ INGKKTKEIH RVKRASDKKV SSKRVSRTWK IPKYPKKQPK
SDQEHPGFNL DYMQPTTHPP HHN
