ID   RGF1B_BOVIN             Reviewed;         472 AA.
AC   A4IFE4;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Ras-GEF domain-containing family member 1B;
GN   Name=RASGEF1B;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Thalamus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Guanine nucleotide exchange factor (GEF) with specificity for
CC       RAP2A, it doesn't seems to activate other Ras family proteins (in
CC       vitro). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with CCDC124 during cytokinesis. Interacts with Ras
CC       family proteins (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Early endosome {ECO:0000250}. Late endosome
CC       {ECO:0000250}. Midbody {ECO:0000250}. Note=Localizes to midbody at
CC       telophase (By similarity). Localizes to midbody at telophase (By
CC       similarity). May shuttle between early and late endosomes. Does not
CC       colocalize with lysosomal markers (By similarity). {ECO:0000250}.
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DR   EMBL; BC134542; AAI34543.1; -; mRNA.
DR   RefSeq; NP_001077118.1; NM_001083649.1.
DR   AlphaFoldDB; A4IFE4; -.
DR   SMR; A4IFE4; -.
DR   STRING; 9913.ENSBTAP00000042728; -.
DR   PaxDb; A4IFE4; -.
DR   PRIDE; A4IFE4; -.
DR   Ensembl; ENSBTAT00000045329; ENSBTAP00000042728; ENSBTAG00000006561.
DR   GeneID; 506971; -.
DR   KEGG; bta:506971; -.
DR   CTD; 153020; -.
DR   VEuPathDB; HostDB:ENSBTAG00000006561; -.
DR   VGNC; VGNC:33744; RASGEF1B.
DR   eggNOG; KOG3417; Eukaryota.
DR   eggNOG; KOG3541; Eukaryota.
DR   GeneTree; ENSGT00940000155816; -.
DR   HOGENOM; CLU_022907_3_0_1; -.
DR   InParanoid; A4IFE4; -.
DR   OMA; THEYYPD; -.
DR   OrthoDB; 533115at2759; -.
DR   TreeFam; TF313379; -.
DR   Proteomes; UP000009136; Chromosome 6.
DR   Bgee; ENSBTAG00000006561; Expressed in bone marrow and 101 other tissues.
DR   GO; GO:0005769; C:early endosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0005770; C:late endosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0030496; C:midbody; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR   GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR   CDD; cd00155; RasGEF; 1.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   PANTHER; PTHR23113; PTHR23113; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   Pfam; PF00618; RasGEF_N; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SMART; SM00229; RasGEFN; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   PROSITE; PS00720; RASGEF; 1.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
PE   2: Evidence at transcript level;
KW   Endosome; Guanine-nucleotide releasing factor; Reference proteome.
FT   CHAIN           1..472
FT                   /note="Ras-GEF domain-containing family member 1B"
FT                   /id="PRO_0000297637"
FT   DOMAIN          34..164
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT   DOMAIN          204..452
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
SQ   SEQUENCE   472 AA;  55127 MW;  8C0D49F42CF5AA05 CRC64;
     MPQTPPFSAM FDSSGYNRNL YQSAEDSCGG LYYHDNNLLS GSLEALIQHL VPNVDYYPDR
     TYIFTFLLSS RLFMHPYELM AKVCHLCVEH QRLSDPNSDK NQIRKIAPKI LQLLTEWTET
     FPYDFRDERM MRNLKDLAHR IASGEETYRK NVQQMIQCLI RKLAALSQYE EVLAKISSTS
     TDRLTVLKTK PQSIQRDIIT VCSDPYTLAQ QLTHIELERL NYIGPEEFVQ AFVQKDPLDN
     DKSCYSERKK TRNLEAYVEW FNRLSYLVAT EICMPVKKKH RARMIEYFID VARECFNIGN
     FNSLMAIISG MNMSPVSRLK KTWAKVKTAK FDVLEHQMDP SSNFYNYRTA LRGAAQRSLT
     AHSSREKIVI PFFSLLIKDI YFLNEGCANR LPNGHVNFEK FWELAKQVSE FMTWKQVECP
     FERDRKILQH LLTVPVFSED ALYLASYESE GPENHIEKDR WKSLRSSLLG RV