RGF1B_MOUSE
ID RGF1B_MOUSE Reviewed; 473 AA.
AC Q8JZL7; F5H8M5; Q3UE12; Q8C189;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Ras-GEF domain-containing family member 1B;
DE AltName: Full=GPI gamma-4;
GN Name=Rasgef1b; Synonyms=Gpig4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), TISSUE SPECIFICITY,
RP AND INDUCTION.
RC STRAIN=C3H/HeJ;
RX PubMed=12488504;
RA Ferreira L.R.P., Abrantes E.F., Rodrigues C.V., Caetano B., Cerqueira G.C.,
RA Salim A.C., Reis L.F.L., Gazzinelli R.T.;
RT "Identification and characterization of a novel mouse gene encoding a Ras-
RT associated guanine nucleotide exchange factor: expression in macrophages
RT and myocarditis elicited by Trypanosoma cruzi parasites.";
RL J. Leukoc. Biol. 72:1215-1227(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J;
RC TISSUE=Bone marrow macrophage {ECO:0000312|EMBL:BAE29099.1},
RC Cerebellum {ECO:0000312|EMBL:AK048739}, and
RC Skin {ECO:0000312|EMBL:BAC26101.1};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION, INTERACTION WITH RAS, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=20090772; DOI=10.1038/gene.2009.107;
RA Andrade W.A., Silva A.M., Alves V.S., Salgado A.P., Melo M.B.,
RA Andrade H.M., Dall'Orto F.V., Garcia S.A., Silveira T.N., Gazzinelli R.T.;
RT "Early endosome localization and activity of RasGEF1b, a toll-like
RT receptor-inducible Ras guanine-nucleotide exchange factor.";
RL Genes Immun. 11:447-457(2010).
CC -!- FUNCTION: Guanine nucleotide exchange factor (GEF) with specificity for
CC RAP2A, it doesn't seems to activate other Ras family proteins (in
CC vitro). {ECO:0000269|PubMed:20090772}.
CC -!- SUBUNIT: Interacts with CCDC124 during cytokinesis (By similarity).
CC Interacts with Ras family proteins. {ECO:0000250,
CC ECO:0000269|PubMed:20090772}.
CC -!- SUBCELLULAR LOCATION: Early endosome {ECO:0000269|PubMed:20090772}.
CC Late endosome {ECO:0000269|PubMed:20090772}. Midbody {ECO:0000250}.
CC Note=Localizes to midbody at telophase (By similarity). May shuttle
CC between early and late endosomes. Does not colocalize with lysosomal
CC markers. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8JZL7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8JZL7-2; Sequence=VSP_027315;
CC Name=3;
CC IsoId=Q8JZL7-3; Sequence=VSP_059002, VSP_059003;
CC -!- TISSUE SPECIFICITY: Constitutively expressed in brain, intestine and
CC testis. Low constitutive expression, if any, in heart, lung, lymph
CC nodes and thymus. Up-regulated in heart, kidney, liver, lymph nodes,
CC spleen and thymus at day 20 after infection with Trypanosoma cruzi. Not
CC detected in muscle. {ECO:0000269|PubMed:12488504}.
CC -!- INDUCTION: In vitro, up-regulated in peritoneal macrophages by GPI-
CC mucins, bacterial lipopolysaccharides (LPS) and poly(I:C). Small
CC induction, if any, by IFNG alone. Induction is maximal 12 and 18 hours
CC following LPS stimulation (at protein level). Also induced in T-helper
CC cells activated by phytohemagglutinin. In vivo, up-regulated by
CC infection with protozoan parasites, including Plasmodium chabaudi and
CC Trypanosoma cruzi. This induction is dependent upon IFNG, MYD88 and
CC TICAM1. {ECO:0000269|PubMed:12488504, ECO:0000269|PubMed:20090772}.
CC -!- SEQUENCE CAUTION: [Isoform 3]:
CC Sequence=AK048739; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY129963; AAM77973.1; -; mRNA.
DR EMBL; AY129964; AAM77974.1; -; Genomic_DNA.
DR EMBL; AK028756; BAC26101.1; -; mRNA.
DR EMBL; AK048739; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK149812; BAE29099.1; -; mRNA.
DR EMBL; AC121578; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC122348; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC125319; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC132257; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC132386; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC050858; AAH50858.1; -; mRNA.
DR EMBL; BC052726; AAH52726.1; -; mRNA.
DR CCDS; CCDS39179.1; -. [Q8JZL7-1]
DR CCDS; CCDS51570.1; -. [Q8JZL7-2]
DR RefSeq; NP_665838.1; NM_145839.2. [Q8JZL7-1]
DR RefSeq; NP_851835.1; NM_181318.4. [Q8JZL7-2]
DR AlphaFoldDB; Q8JZL7; -.
DR SMR; Q8JZL7; -.
DR BioGRID; 235908; 19.
DR STRING; 10090.ENSMUSP00000031276; -.
DR iPTMnet; Q8JZL7; -.
DR PhosphoSitePlus; Q8JZL7; -.
DR MaxQB; Q8JZL7; -.
DR PaxDb; Q8JZL7; -.
DR PRIDE; Q8JZL7; -.
DR ProteomicsDB; 254929; -. [Q8JZL7-1]
DR ProteomicsDB; 254930; -. [Q8JZL7-2]
DR ProteomicsDB; 254931; -. [Q8JZL7-3]
DR Antibodypedia; 25022; 86 antibodies from 21 providers.
DR DNASU; 320292; -.
DR Ensembl; ENSMUST00000031276; ENSMUSP00000031276; ENSMUSG00000089809. [Q8JZL7-1]
DR Ensembl; ENSMUST00000146396; ENSMUSP00000125723; ENSMUSG00000089809. [Q8JZL7-3]
DR Ensembl; ENSMUST00000168092; ENSMUSP00000129652; ENSMUSG00000089809. [Q8JZL7-2]
DR GeneID; 320292; -.
DR KEGG; mmu:320292; -.
DR UCSC; uc008ygl.1; mouse. [Q8JZL7-1]
DR UCSC; uc008ygm.1; mouse. [Q8JZL7-2]
DR CTD; 153020; -.
DR MGI; MGI:2443755; Rasgef1b.
DR VEuPathDB; HostDB:ENSMUSG00000089809; -.
DR eggNOG; KOG3417; Eukaryota.
DR eggNOG; KOG3541; Eukaryota.
DR GeneTree; ENSGT00940000155816; -.
DR InParanoid; Q8JZL7; -.
DR OrthoDB; 533115at2759; -.
DR PhylomeDB; Q8JZL7; -.
DR TreeFam; TF313379; -.
DR BioGRID-ORCS; 320292; 0 hits in 70 CRISPR screens.
DR ChiTaRS; Rasgef1b; mouse.
DR PRO; PR:Q8JZL7; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8JZL7; protein.
DR Bgee; ENSMUSG00000089809; Expressed in interventricular septum and 200 other tissues.
DR ExpressionAtlas; Q8JZL7; baseline and differential.
DR Genevisible; Q8JZL7; MM.
DR GO; GO:0005769; C:early endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005770; C:late endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0030496; C:midbody; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS00720; RASGEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Endosome; Guanine-nucleotide releasing factor;
KW Reference proteome.
FT CHAIN 1..473
FT /note="Ras-GEF domain-containing family member 1B"
FT /id="PRO_0000297639"
FT DOMAIN 34..161
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 205..453
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT VAR_SEQ 1..116
FT /note="MPQTPPFSAMFDSSGYNRNLYQSAEDSCGGLYYHDNNLLSGSLEALIQHLVP
FT NVDYYPDRTYIFTFLLSSRLFMHPYELMAKVCHLCVEHQRLSEGDGDKNQMRKIAPKIL
FT QLLTE -> MAKFLAALLGCTLPSKGASPVLEGTIFSTLAGEKSSSDYSKEQQWKPVYC
FT SLAAMCLSNPLIEKPGGKIPTKTPNISALPLDIFIHPLKTFILPRSFVQTATSETTHFA
FT CLSMSWS (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_059002"
FT VAR_SEQ 117..473
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_059003"
FT VAR_SEQ 147
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_027315"
FT CONFLICT 436
FT /note="P -> H (in Ref. 2; BAE29099)"
FT /evidence="ECO:0000305"
FT CONFLICT Q8JZL7-3:71
FT /note="T -> N (in Ref. 2; AK048739)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 473 AA; 55273 MW; 9F6E8868E58A36DE CRC64;
MPQTPPFSAM FDSSGYNRNL YQSAEDSCGG LYYHDNNLLS GSLEALIQHL VPNVDYYPDR
TYIFTFLLSS RLFMHPYELM AKVCHLCVEH QRLSEGDGDK NQMRKIAPKI LQLLTEWTET
FPYDFRDERM MRNLKDLAHR MASGEEQTYR KNVQQMMQCL IRKLAALSQY EEVLAKLSST
ATDRLTVLKT KPQSIQRDIM TVCSDPYTLA QQLTHIELER LNYIGPEEFV QAFVQKDPLD
NDKSCYSERK KTRNLEAYVE WFNRLSYLVA TEICMPVKKK HRARMIEYFI DVARECFNIG
NFNSLMAIIS GMNMSPVSRL KKTWAKVKTA KFDILEHQMD PSSNFYNYRT ALRGAAQRSL
TAHSSREKIV IPFFSLLIKD IYFLNEGCVN RLPNGHVNFE KFWELAKQVS EFMTWKQVEC
PFERDRKVLQ YLLSVPVFSE DALYLASYES EGPENNIEKD RWKSLRSSLL GRV
