RGI1_YEAST
ID RGI1_YEAST Reviewed; 161 AA.
AC P40043; D3DLX2;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Respiratory growth induced protein 1;
GN Name=RGI1; OrderedLocusNames=YER067W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP INDUCTION.
RX PubMed=10770760; DOI=10.1128/aac.44.5.1255-1265.2000;
RA Bammert G.F., Fostel J.M.;
RT "Genome-wide expression patterns in Saccharomyces cerevisiae: comparison of
RT drug treatments and genetic alterations affecting biosynthesis of
RT ergosterol.";
RL Antimicrob. Agents Chemother. 44:1255-1265(2000).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-68, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=SUB592;
RX PubMed=12872131; DOI=10.1038/nbt849;
RA Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G.,
RA Roelofs J., Finley D., Gygi S.P.;
RT "A proteomics approach to understanding protein ubiquitination.";
RL Nat. Biotechnol. 21:921-926(2003).
RN [7]
RP INDUCTION.
RX PubMed=15870279; DOI=10.1128/mcb.25.10.4075-4091.2005;
RA Lai L.C., Kosorukoff A.L., Burke P.V., Kwast K.E.;
RT "Dynamical remodeling of the transcriptome during short-term anaerobiosis
RT in Saccharomyces cerevisiae: differential response and role of Msn2 and/or
RT Msn4 and other factors in galactose and glucose media.";
RL Mol. Cell. Biol. 25:4075-4091(2005).
RN [8]
RP INDUCTION.
RX PubMed=18756096;
RA Seo H.Y., Chang Y.J., Chung Y.J., Kim K.S.;
RT "Proteomic analysis of recombinant Saccharomyces cerevisiae upon iron
RT deficiency induced via human H-ferritin production.";
RL J. Microbiol. Biotechnol. 18:1368-1376(2008).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 11-161, FUNCTION, AND SUBCELLULAR
RP LOCATION.
RX PubMed=20567505; DOI=10.1371/journal.pone.0011163;
RA Domitrovic T., Kozlov G., Freire J.C., Masuda C.A., da Silva Almeida M.,
RA Montero-Lomeli M., Atella G.C., Matta-Camacho E., Gehring K.,
RA Kurtenbach E.;
RT "Structural and functional study of YER067W, a new protein involved in
RT yeast metabolism control and drug resistance.";
RL PLoS ONE 5:E11163-E11163(2010).
CC -!- FUNCTION: Involved in the control of energetic metabolism and
CC significantly contribute to cell fitness, especially under respiratory
CC growth conditions. {ECO:0000269|PubMed:20567505}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20567505};
CC Peripheral membrane protein {ECO:0000269|PubMed:20567505}.
CC -!- INDUCTION: Up-regulated by a wide range of conditions, such as
CC intracellular iron depletion, carbon source restriction, high
CC temperature, high osmotic stress, cold stress, unfolded protein
CC response, and high hydrostatic pressure. The promoter contains several
CC binding sites for the stress response transcription factors MSN2, MSN4
CC and HSF1. Down-regulated when treated with different antifungal drugs
CC from the azole class. {ECO:0000269|PubMed:10770760,
CC ECO:0000269|PubMed:15870279, ECO:0000269|PubMed:18756096}.
CC -!- MISCELLANEOUS: Present with 8450 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the RGI1 family. {ECO:0000305}.
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DR EMBL; U18813; AAB64603.1; -; Genomic_DNA.
DR EMBL; AY692724; AAT92743.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07726.1; -; Genomic_DNA.
DR PIR; S50570; S50570.
DR RefSeq; NP_010990.1; NM_001178958.1.
DR PDB; 3BCY; X-ray; 1.70 A; A=11-161.
DR PDBsum; 3BCY; -.
DR AlphaFoldDB; P40043; -.
DR SMR; P40043; -.
DR BioGRID; 36810; 49.
DR DIP; DIP-5346N; -.
DR IntAct; P40043; 2.
DR MINT; P40043; -.
DR STRING; 4932.YER067W; -.
DR iPTMnet; P40043; -.
DR MaxQB; P40043; -.
DR PaxDb; P40043; -.
DR PRIDE; P40043; -.
DR EnsemblFungi; YER067W_mRNA; YER067W; YER067W.
DR GeneID; 856797; -.
DR KEGG; sce:YER067W; -.
DR SGD; S000000869; RGI1.
DR VEuPathDB; FungiDB:YER067W; -.
DR eggNOG; ENOG502RZ9F; Eukaryota.
DR GeneTree; ENSGT00940000176408; -.
DR HOGENOM; CLU_118207_0_0_1; -.
DR InParanoid; P40043; -.
DR OMA; YAEETEY; -.
DR BioCyc; YEAST:G3O-30241-MON; -.
DR ChiTaRS; RGI1; yeast.
DR EvolutionaryTrace; P40043; -.
DR PRO; PR:P40043; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P40043; protein.
DR GO; GO:0071944; C:cell periphery; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006112; P:energy reserve metabolic process; IMP:SGD.
DR Gene3D; 3.40.1000.40; -; 1.
DR InterPro; IPR022554; RGI1.
DR InterPro; IPR038235; RGI1_sf.
DR Pfam; PF10843; RGI1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Isopeptide bond; Membrane; Reference proteome;
KW Ubl conjugation.
FT CHAIN 1..161
FT /note="Respiratory growth induced protein 1"
FT /id="PRO_0000202633"
FT CROSSLNK 68
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000269|PubMed:12872131"
FT STRAND 17..27
FT /evidence="ECO:0007829|PDB:3BCY"
FT HELIX 28..40
FT /evidence="ECO:0007829|PDB:3BCY"
FT STRAND 48..53
FT /evidence="ECO:0007829|PDB:3BCY"
FT HELIX 56..60
FT /evidence="ECO:0007829|PDB:3BCY"
FT TURN 61..64
FT /evidence="ECO:0007829|PDB:3BCY"
FT HELIX 66..68
FT /evidence="ECO:0007829|PDB:3BCY"
FT HELIX 78..90
FT /evidence="ECO:0007829|PDB:3BCY"
FT HELIX 92..99
FT /evidence="ECO:0007829|PDB:3BCY"
FT STRAND 108..114
FT /evidence="ECO:0007829|PDB:3BCY"
FT STRAND 116..129
FT /evidence="ECO:0007829|PDB:3BCY"
FT STRAND 132..145
FT /evidence="ECO:0007829|PDB:3BCY"
FT STRAND 149..159
FT /evidence="ECO:0007829|PDB:3BCY"
SQ SEQUENCE 161 AA; 18989 MW; 31C2929EBB19BF37 CRC64;
MTKKDKKEVK VQTVTTEDGE TVKVFEDLQG FETFIANETE DDDFDHLHCK LNYYPPFVLH
ESHEDPEKIS DAANSHSKKF VRHLHQHIEK HLLKDIKQAV RKPELKFHEK SKEETFDKIT
WHYGEETEYH GRPFKIDVQV VCTHEDAMVF VDYKTHPVGA N
