RGL1_MOUSE
ID RGL1_MOUSE Reviewed; 768 AA.
AC Q60695; Q8VD09;
DT 20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Ral guanine nucleotide dissociation stimulator-like 1;
DE Short=RalGDS-like 1;
GN Name=Rgl1; Synonyms=Rgl;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7935463; DOI=10.1128/mcb.14.11.7483-7491.1994;
RA Kikuchi A., Demo S.D., Ye Z.H., Chen Y.W., Williams L.T.;
RT "ralGDS family members interact with the effector loop of ras p21.";
RL Mol. Cell. Biol. 14:7483-7491(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Amnion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-520, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP STRUCTURE BY NMR OF 632-734.
RX PubMed=9891982; DOI=10.1016/s0014-5793(98)01596-8;
RA Kigawa T., Endo M., Ito Y., Shirouzu M., Kikuchi A., Yokoyama S.;
RT "Solution structure of the Ras-binding domain of RGL.";
RL FEBS Lett. 441:413-418(1998).
CC -!- FUNCTION: Probable guanine nucleotide exchange factor.
CC -!- SUBUNIT: Interacts with Ras.
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DR EMBL; U14103; AAA64950.1; -; mRNA.
DR EMBL; AK144218; BAE25780.1; -; mRNA.
DR EMBL; AK169109; BAE40891.1; -; mRNA.
DR EMBL; AK169180; BAE40958.1; -; mRNA.
DR EMBL; CH466520; EDL39452.1; -; Genomic_DNA.
DR EMBL; BC017678; AAH17678.1; -; mRNA.
DR CCDS; CCDS15365.1; -.
DR PIR; A56234; A56234.
DR RefSeq; NP_001333046.1; NM_001346117.1.
DR RefSeq; NP_001333047.1; NM_001346118.1.
DR RefSeq; NP_001333048.1; NM_001346119.1.
DR RefSeq; NP_058542.2; NM_016846.4.
DR PDB; 1EF5; NMR; -; A=632-734.
DR PDBsum; 1EF5; -.
DR AlphaFoldDB; Q60695; -.
DR SMR; Q60695; -.
DR STRING; 10090.ENSMUSP00000027760; -.
DR iPTMnet; Q60695; -.
DR PhosphoSitePlus; Q60695; -.
DR MaxQB; Q60695; -.
DR PaxDb; Q60695; -.
DR PRIDE; Q60695; -.
DR ProteomicsDB; 253257; -.
DR Antibodypedia; 20603; 139 antibodies from 28 providers.
DR DNASU; 19731; -.
DR Ensembl; ENSMUST00000027760; ENSMUSP00000027760; ENSMUSG00000026482.
DR GeneID; 19731; -.
DR KEGG; mmu:19731; -.
DR UCSC; uc007czh.1; mouse.
DR CTD; 23179; -.
DR MGI; MGI:107484; Rgl1.
DR VEuPathDB; HostDB:ENSMUSG00000026482; -.
DR eggNOG; KOG3629; Eukaryota.
DR GeneTree; ENSGT00940000156012; -.
DR HOGENOM; CLU_010252_0_1_1; -.
DR InParanoid; Q60695; -.
DR OMA; EQFNGLY; -.
DR TreeFam; TF315204; -.
DR Reactome; R-MMU-5673001; RAF/MAP kinase cascade.
DR BioGRID-ORCS; 19731; 3 hits in 71 CRISPR screens.
DR ChiTaRS; Rgl1; mouse.
DR EvolutionaryTrace; Q60695; -.
DR PRO; PR:Q60695; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q60695; protein.
DR Bgee; ENSMUSG00000026482; Expressed in right kidney and 220 other tissues.
DR ExpressionAtlas; Q60695; baseline and differential.
DR Genevisible; Q60695; MM.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR030748; RGL1.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR PANTHER; PTHR23113:SF199; PTHR23113:SF199; 1.
DR Pfam; PF00788; RA; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00314; RA; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS00720; RASGEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Guanine-nucleotide releasing factor; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..768
FT /note="Ral guanine nucleotide dissociation stimulator-like
FT 1"
FT /id="PRO_0000068886"
FT DOMAIN 65..196
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 232..501
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT DOMAIN 648..735
FT /note="Ras-associating"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00166"
FT REGION 530..623
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 531..565
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 583..623
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 520
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 662
FT /note="N -> H (in Ref. 1; AAA64950)"
FT /evidence="ECO:0000305"
FT STRAND 652..658
FT /evidence="ECO:0007829|PDB:1EF5"
FT HELIX 676..682
FT /evidence="ECO:0007829|PDB:1EF5"
FT TURN 683..687
FT /evidence="ECO:0007829|PDB:1EF5"
FT STRAND 692..701
FT /evidence="ECO:0007829|PDB:1EF5"
FT STRAND 703..705
FT /evidence="ECO:0007829|PDB:1EF5"
FT STRAND 708..711
FT /evidence="ECO:0007829|PDB:1EF5"
FT TURN 716..720
FT /evidence="ECO:0007829|PDB:1EF5"
FT STRAND 726..732
FT /evidence="ECO:0007829|PDB:1EF5"
SQ SEQUENCE 768 AA; 86324 MW; F59853A281DD52F6 CRC64;
MKLLWQAKMS SIQDWGEEVE EGAVYHVTLK RVQIQQAANK GARWLGVEGD QLPPGHTVSQ
YETCKIRTIK AGTLEKLVEN LLTAFGDNDF TYISIFLSTY RGFASTKEVL ELLLDRYGNL
TGPNCEDDGS QSSPESKAVI RNAIASILRA WLDQCAEDFR EPPHFPCLQK LLEYLKQMMP
GSDPERRAQN LLEQFQKQDV DSDNGLLNTS SFSLEEEEEL ESGGSAEFTN FSEDLVAEQL
TYMDAQLFKK VVPHHCLGCI WSQRDKKENK HLAPTIRATI SQFNTLTKCV VSTVLGSKEL
KTQQRARVIE KWINIAHECR ILKNFSSLRA IVSALQSNSI YRLKKAWAAV PKDRMLMFEE
LSDIFSDHNN HLTSRELLMK EGTSKFANLD SSVKENQKRT QRRLQLQKDM GVMQGTVPYL
GTFLTDLTML DTALQDYIEG GLINFEKRRR EFEVIAQIKL LQSACNSYCM GPDQKFIQWF
QRQQLLSEEE SYALSCEIEA AADANTTSPK PRKSMVKRLS LLFLGSDIIP GSTPTKEQPK
SAASGSSGES MDSVSVSSCE SNHSEAEEGP VTPMDTPDEP QKKLSESSSS CSSIHSMDTN
SSGMSSLINP LSSPPTCNNN PKIHKRSVSV TSITSTVLPP VYNQQNEDTC IIRISVEDNN
GNMYKSIMLT SQDKTPAVIQ RAMSKHNLES DPAEEYELVQ VISEDKELVI PDSANVFYAM
NSQVNFDFIL RKKNSVEEQV KLRSRTSLTL PRTAKRGCWS NRHSKITL
