RGL1_YEAST
ID RGL1_YEAST Reviewed; 479 AA.
AC Q12194; D6W3U9;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=RHO1 GEF localizing protein 1 {ECO:0000303|PubMed:22344253};
GN Name=RGL1 {ECO:0000303|PubMed:22344253}; OrderedLocusNames=YPL066W;
GN ORFNames=LPE4W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22344253; DOI=10.1242/jcs.100685;
RA Krause S.A., Cundell M.J., Poon P.P., McGhie J., Johnston G.C., Price C.,
RA Gray J.V.;
RT "Functional specialisation of yeast Rho1 GTP exchange factors.";
RL J. Cell Sci. 125:2721-2731(2012).
CC -!- FUNCTION: Regulator of RHO1 signaling that acts as a cofactor required
CC for the efficient localization of the TUS1 GTP exchange factor (GEF)
CC for RHO1 to the bud neck during all phases of cytokinesis
CC (PubMed:22344253). RHO1 is a key, essential hub protein in the cell
CC wall integrity (CWI) pathway in which activated RHO1-GTP binds directly
CC to and activates multiple different downstream effectors required for
CC cell wall synthesis and actin assembly during cytokinesis (Probable).
CC {ECO:0000269|PubMed:22344253, ECO:0000305|PubMed:22344253}.
CC -!- DISRUPTION PHENOTYPE: Impairs the efficient localization of TUS1 to the
CC bud neck at late anaphase. {ECO:0000269|PubMed:22344253}.
CC -!- MISCELLANEOUS: Present with 396 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U50630; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U39205; AAB68299.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11365.1; -; Genomic_DNA.
DR PIR; S60924; S60924.
DR RefSeq; NP_015259.1; NM_001183880.1.
DR AlphaFoldDB; Q12194; -.
DR BioGRID; 36113; 198.
DR DIP; DIP-2817N; -.
DR IntAct; Q12194; 3.
DR MINT; Q12194; -.
DR STRING; 4932.YPL066W; -.
DR iPTMnet; Q12194; -.
DR PaxDb; Q12194; -.
DR PRIDE; Q12194; -.
DR EnsemblFungi; YPL066W_mRNA; YPL066W; YPL066W.
DR GeneID; 856039; -.
DR KEGG; sce:YPL066W; -.
DR SGD; S000005987; RGL1.
DR VEuPathDB; FungiDB:YPL066W; -.
DR eggNOG; ENOG502QSRB; Eukaryota.
DR HOGENOM; CLU_598506_0_0_1; -.
DR InParanoid; Q12194; -.
DR OMA; NSVIRGC; -.
DR BioCyc; YEAST:G3O-33975-MON; -.
DR PRO; PR:Q12194; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q12194; protein.
DR GO; GO:0005935; C:cellular bud neck; HDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0000935; C:division septum; IBA:GO_Central.
DR GO; GO:0000917; P:division septum assembly; IBA:GO_Central.
DR GO; GO:0097271; P:protein localization to bud neck; IMP:SGD.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..479
FT /note="RHO1 GEF localizing protein 1"
FT /id="PRO_0000203492"
FT REGION 460..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 479 AA; 54893 MW; D6C9FA7AAEBACF74 CRC64;
MTHPVISLKP SYNSVIRGCP GLPDTLPRIE CQLRVRSNNS LPFKLVKIEI VLKTIEIYFN
KNLYSSNNSS FTPFNRPSDP SNGHSDTSNQ NISIHYKKNI VLSHPTHDGD DLNNDLIGID
IPLTIGLPDD IKETNYNPKF GKTQTFLDCT VFYTEVGGGS SNKKRNFLYP VNVERYTYLP
SPSYFRPINR SNITSPDQKF LISYSIENPC VSMNNDTLKL SISIRLNPFP NNATTPSSND
FDVSTPTLFS TKKKFKSKLK LKSITTQILE YLEILKNQSE FSSTQTTNIL QTSVRQVDQI
ISMNSMIFQF NLKIFTKDKI LQSFRSSESS CPETKVLINK IDDIPLQYHS SITTIGQHFN
VSHYLSIRFK FNKSLKNFEI NHPLIISFWS VSQLPLIENL ILQERQTAKF AKKFYKNFGR
IKNTSNNNNS SNCLEYPSLP PIIYNFNDPE TNNRFNILYS QKDPSRTDPS KLRRVPVIQ
