ID   RGLT_EMENI              Reviewed;         421 AA.
AC   A0A1U8QZJ7; C8VRW1; Q5BDL2;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2017, sequence version 1.
DT   03-AUG-2022, entry version 24.
DE   RecName: Full=Transcription factor rglT {ECO:0000303|PubMed:32667960};
GN   Name=rglT {ECO:0000303|PubMed:32667960}; ORFNames=AN1368, ANIA_01368;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=32667960; DOI=10.1371/journal.ppat.1008645;
RA   Ries L.N.A., Pardeshi L., Dong Z., Tan K., Steenwyk J.L., Colabardini A.C.,
RA   Ferreira Filho J.A., de Castro P.A., Silva L.P., Preite N.W., Almeida F.,
RA   de Assis L.J., Dos Santos R.A.C., Bowyer P., Bromley M., Owens R.A.,
RA   Doyle S., Demasi M., Hernandez D.C.R., Netto L.E.S., Pupo M.T., Rokas A.,
RA   Loures F.V., Wong K.H., Goldman G.H.;
RT   "The Aspergillus fumigatus transcription factor RglT is important for
RT   gliotoxin biosynthesis and self-protection, and virulence.";
RL   PLoS Pathog. 16:e1008645-e1008645(2020).
CC   -!- FUNCTION: Transcription factor that is important for oxidative stress
CC       resistance and essential for gliotoxin (GT) self-protection through the
CC       regulation of a gene encoding a putative gliT homolog, even if
CC       E.nidulans does not produce gliotoxin itself.
CC       {ECO:0000269|PubMed:32667960}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- DISRUPTION PHENOTYPE: Abolishes growth in the presence of gliotoxin
CC       (PubMed:32667960). Leads to reduced growth in the presence of the
CC       oxidative stress-inducing compounds allyl alcohol, t-butyl
CC       hydroperoxide, but not menadione (PubMed:32667960).
CC       {ECO:0000269|PubMed:32667960}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BN001308; CBF87636.1; -; Genomic_DNA.
DR   EMBL; AACD01000018; EAA65551.1; -; Genomic_DNA.
DR   RefSeq; XP_658972.1; XM_653880.1.
DR   EnsemblFungi; CBF87636; CBF87636; ANIA_01368.
DR   EnsemblFungi; EAA65551; EAA65551; AN1368.2.
DR   GeneID; 2877143; -.
DR   KEGG; ani:AN1368.2; -.
DR   VEuPathDB; FungiDB:AN1368; -.
DR   eggNOG; ENOG502SE9E; Eukaryota.
DR   HOGENOM; CLU_026660_1_0_1; -.
DR   OMA; PKRHAAC; -.
DR   OrthoDB; 740957at2759; -.
DR   Proteomes; UP000000560; Chromosome VIII.
DR   Proteomes; UP000005890; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..421
FT                   /note="Transcription factor rglT"
FT                   /id="PRO_0000454488"
FT   DNA_BIND        38..65
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          346..371
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        346..360
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   421 AA;  46777 MW;  DB75FBF79DBF32DB CRC64;
     MQYEAYQWGQ SHPTSTSGSM LQDTPTAASQ PVKRHAACDE CRKRKLKCSG EISGCSRCIK
     QSLSCHYSVQ KQMGRPPKKR LREDNDDISL LNIPDNDPWA NSQIAHVPDF GAAIADVSEE
     PQILSSTHLS PYSFPYRLST DEDHRHTWQL APNESMSSIP ATTTPWPDFS SVSAAAPKPF
     IMPPGLTPPI MPSTDSSPDQ ECSCLSYLYL CLSHLSSLKP FPISQHTICS LYISAKTAQS
     VIRCQSCPNR FDTGLQNVMF TGTLLNVIAD SWLRVSRAEA CELGNQVAPP AYAAKMNRSP
     DLRGAWNDYL RQLVRFSVIR GPMDIDAQTP CAQQAPSVLD LVEEMEARQR RWHESPDSHP
     LPPDQRLNIP SDQNCLNRDE QDLLCIRVAK SARNVIAKFG FQSDEYPESV PSLSPDSSIS
     P