RGMB_MOUSE
ID RGMB_MOUSE Reviewed; 436 AA.
AC Q7TQ33; Q501K0; Q8BNR6; Q8CBM7;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Repulsive guidance molecule B {ECO:0000303|PubMed:15053976};
DE AltName: Full=DRG11-responsive axonal guidance and outgrowth of neurite {ECO:0000303|PubMed:14985445};
DE Short=DRAGON {ECO:0000303|PubMed:14985445};
DE Flags: Precursor;
GN Name=Rgmb {ECO:0000303|PubMed:15053976, ECO:0000312|MGI:MGI:1916049};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=14678836; DOI=10.1016/s1567-133x(03)00144-3;
RA Schmidtmer J., Engelkamp D.;
RT "Isolation and expression pattern of three mouse homologues of chick Rgm.";
RL Gene Expr. Patterns 4:105-110(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Corpora quadrigemina, and Urinary bladder;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=15053976; DOI=10.1016/j.modgep.2003.11.008;
RA Oldekamp J., Kraemer N., Alvarez-Bolado G., Skutella T.;
RT "Expression pattern of the repulsive guidance molecules RGM A, B and C
RT during mouse development.";
RL Gene Expr. Patterns 4:283-288(2004).
RN [5]
RP DEVELOPMENTAL STAGE.
RX PubMed=14749425; DOI=10.1523/jneurosci.4610-03.2004;
RA Niederkofler V., Salie R., Sigrist M., Arber S.;
RT "Repulsive guidance molecule (RGM) gene function is required for neural
RT tube closure but not retinal topography in the mouse visual system.";
RL J. Neurosci. 24:808-818(2004).
RN [6]
RP FUNCTION, SUBUNIT, INTERACTION WITH DRGX, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND GPI-ANCHOR.
RX PubMed=14985445; DOI=10.1523/jneurosci.4115-03.2004;
RA Samad T.A., Srinivasan A., Karchewski L.A., Jeong S.-J., Campagna J.A.,
RA Ji R.-R., Fabrizio D.A., Zhang Y., Lin H.Y., Bell E., Woolf C.J.;
RT "DRAGON: a member of the repulsive guidance molecule-related family of
RT neuronal- and muscle-expressed membrane proteins is regulated by DRG11 and
RT has neuronal adhesive properties.";
RL J. Neurosci. 24:2027-2036(2004).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=15890774; DOI=10.1210/en.2004-1676;
RA Xia Y., Sidis Y., Mukherjee A., Samad T.A., Brenner G., Woolf C.J.,
RA Lin H.Y., Schneyer A.;
RT "Localization and action of Dragon (repulsive guidance molecule b), a novel
RT bone morphogenetic protein coreceptor, throughout the reproductive axis.";
RL Endocrinology 146:3614-3621(2005).
RN [8]
RP FUNCTION, INTERACTION WITH ACVR1; ACVR2B; BMP2; BMP4; BMPR1A AND BMPR1B,
RP AND DEVELOPMENTAL STAGE.
RX PubMed=15671031; DOI=10.1074/jbc.m410034200;
RA Samad T.A., Rebbapragada A., Bell E., Zhang Y., Sidis Y., Jeong S.-J.,
RA Campagna J.A., Perusini S., Fabrizio D.A., Schneyer A.L., Lin H.Y.,
RA Brivanlou A.H., Attisano L., Woolf C.J.;
RT "DRAGON, a bone morphogenetic protein co-receptor.";
RL J. Biol. Chem. 280:14122-14129(2005).
CC -!- FUNCTION: Member of the repulsive guidance molecule (RGM) family that
CC contributes to the patterning of the developing nervous system. Acts as
CC a bone morphogenetic protein (BMP) coreceptor that potentiates BMP
CC signaling. Promotes neuronal adhesion. May inhibit neurite outgrowth
CC (By similarity). {ECO:0000250|UniProtKB:Q6NW40,
CC ECO:0000269|PubMed:14985445, ECO:0000269|PubMed:15671031,
CC ECO:0000269|PubMed:15890774}.
CC -!- SUBUNIT: Homooligomer. Interacts with DRGX. Interacts with BMP2 and
CC BMP4. Interacts with the BMP type I receptors ACVR1, BMPR1A and BMPR1B
CC and with the BMP type II receptor ACVR2B. The functional complex with
CC its receptor NEO1/neogenin appears to be a heterotetramer with a 2:2
CC stoichiometry, RGM molecules acting as staples that bring two NEO1
CC receptors together without interacting themselves, this arrangement
CC leads to activation of downstream signaling via RhoA.
CC {ECO:0000269|PubMed:14985445, ECO:0000269|PubMed:15671031}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15890774};
CC Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:15890774}. Membrane raft
CC {ECO:0000269|PubMed:15890774}.
CC -!- TISSUE SPECIFICITY: Detected in neonatal and adult dorsal root ganglion
CC sensory neurons, spinal cord, and brain (at protein level). Also
CC expressed at high levels in retinal ganglion cells of developing mouse,
CC extending to the optic nerve (at protein level). Expressed in testis,
CC epididymis, ovary, uterus, and pituitary. {ECO:0000269|PubMed:14985445,
CC ECO:0000269|PubMed:15890774}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the developing nervous system.
CC Expression is restricted to a subset of individual neurons in the
CC mid- and hindbrain regions. At 10.5 dpc, expression level increases and
CC extends further into the forebrain. The segmented pattern of expression
CC becomes more refined and is indicative of peripheral nervous system
CC labelling. Not detected in the area of motoneuron differentiation.
CC Expression could be restricted to postmitotic neurons. Also expressed
CC in fetal dorsal root ganglion, dorsal horn, in the dorsomedial mantle
CC layer of the spinal cord, alar plate of the myelencephalon, marginal
CC layer of the mesencephalon, basal plate of the pons, and cerebellar
CC primordia, as well as the cortex of the olfactory lobe, retina, and
CC olfactory epithelium. In the developing eye, expressed in
CC differentiating ganglion cells and later in the development, also in
CC amacrine cells. In adult, expressed in scattered cells throughout the
CC brain. {ECO:0000269|PubMed:14678836, ECO:0000269|PubMed:14749425,
CC ECO:0000269|PubMed:14985445, ECO:0000269|PubMed:15053976,
CC ECO:0000269|PubMed:15671031}.
CC -!- PTM: GPI-anchored. {ECO:0000269|PubMed:14985445}.
CC -!- PTM: Autocatalytically cleaved at low pH; the two chains remain linked
CC via two disulfide bonds. {ECO:0000250|UniProtKB:Q6NW40}.
CC -!- SIMILARITY: Belongs to the repulsive guidance molecule (RGM) family.
CC {ECO:0000305}.
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DR EMBL; AJ557514; CAD89719.1; -; mRNA.
DR EMBL; AK035713; BAC29163.1; -; mRNA.
DR EMBL; AK080819; BAC38034.1; -; mRNA.
DR EMBL; BC096024; AAH96024.2; -; mRNA.
DR EMBL; BC138890; AAI38891.1; -; mRNA.
DR CCDS; CCDS37455.1; -.
DR RefSeq; NP_848730.2; NM_178615.3.
DR RefSeq; XP_006524928.1; XM_006524865.2.
DR AlphaFoldDB; Q7TQ33; -.
DR SMR; Q7TQ33; -.
DR STRING; 10090.ENSMUSP00000126177; -.
DR GlyGen; Q7TQ33; 2 sites.
DR iPTMnet; Q7TQ33; -.
DR PhosphoSitePlus; Q7TQ33; -.
DR EPD; Q7TQ33; -.
DR PaxDb; Q7TQ33; -.
DR PeptideAtlas; Q7TQ33; -.
DR PRIDE; Q7TQ33; -.
DR ProteomicsDB; 255249; -.
DR Antibodypedia; 2207; 216 antibodies from 30 providers.
DR DNASU; 68799; -.
DR Ensembl; ENSMUST00000170578; ENSMUSP00000126177; ENSMUSG00000048027.
DR GeneID; 68799; -.
DR KEGG; mmu:68799; -.
DR UCSC; uc008aow.1; mouse.
DR CTD; 285704; -.
DR MGI; MGI:1916049; Rgmb.
DR VEuPathDB; HostDB:ENSMUSG00000048027; -.
DR eggNOG; ENOG502QSTJ; Eukaryota.
DR GeneTree; ENSGT00950000183112; -.
DR HOGENOM; CLU_032775_1_1_1; -.
DR InParanoid; Q7TQ33; -.
DR OMA; SQCHEKM; -.
DR OrthoDB; 1300661at2759; -.
DR PhylomeDB; Q7TQ33; -.
DR TreeFam; TF329836; -.
DR BioGRID-ORCS; 68799; 9 hits in 73 CRISPR screens.
DR ChiTaRS; Rgmb; mouse.
DR PRO; PR:Q7TQ33; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q7TQ33; protein.
DR Bgee; ENSMUSG00000048027; Expressed in rostral migratory stream and 286 other tissues.
DR ExpressionAtlas; Q7TQ33; baseline and differential.
DR Genevisible; Q7TQ33; MM.
DR GO; GO:0046658; C:anchored component of plasma membrane; IDA:MGI.
DR GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IDA:MGI.
DR GO; GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015026; F:coreceptor activity; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IDA:MGI.
DR GO; GO:0030509; P:BMP signaling pathway; IDA:MGI.
DR GO; GO:0007155; P:cell adhesion; IDA:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:HGNC-UCL.
DR GO; GO:0007165; P:signal transduction; IDA:HGNC-UCL.
DR InterPro; IPR033608; DRAGON.
DR InterPro; IPR040287; RGM.
DR InterPro; IPR009496; RGM_C.
DR InterPro; IPR010536; RGM_N.
DR PANTHER; PTHR31428; PTHR31428; 1.
DR PANTHER; PTHR31428:SF5; PTHR31428:SF5; 1.
DR Pfam; PF06534; RGM_C; 1.
DR Pfam; PF06535; RGM_N; 1.
PE 1: Evidence at protein level;
KW Autocatalytic cleavage; Cell membrane; Disulfide bond; Glycoprotein;
KW GPI-anchor; Lipoprotein; Membrane; Reference proteome; Signal.
FT SIGNAL 1..48
FT /evidence="ECO:0000255"
FT CHAIN 49..415
FT /note="Repulsive guidance molecule B"
FT /id="PRO_0000030396"
FT PROPEP 416..436
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000030397"
FT SITE 171..172
FT /note="Cleavage; by autolysis"
FT /evidence="ECO:0000250|UniProtKB:Q6NW40"
FT LIPID 415
FT /note="GPI-anchor amidated cysteine"
FT /evidence="ECO:0000255"
FT CARBOHYD 123
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 386
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 142..229
FT /evidence="ECO:0000250|UniProtKB:Q6NW40"
FT DISULFID 166..315
FT /evidence="ECO:0000250|UniProtKB:Q6NW40"
FT CONFLICT 8
FT /note="S -> Y (in Ref. 2; BAC38034)"
FT /evidence="ECO:0000305"
FT CONFLICT 12
FT /note="A -> G (in Ref. 2; BAC38034)"
FT /evidence="ECO:0000305"
FT CONFLICT 15
FT /note="A -> G (in Ref. 2; BAC38034)"
FT /evidence="ECO:0000305"
FT CONFLICT 25
FT /note="G -> R (in Ref. 2; BAC38034)"
FT /evidence="ECO:0000305"
FT CONFLICT 30
FT /note="S -> T (in Ref. 2; BAC38034)"
FT /evidence="ECO:0000305"
FT CONFLICT 141
FT /note="P -> T (in Ref. 2; BAC29163)"
FT /evidence="ECO:0000305"
FT CONFLICT 300
FT /note="E -> K (in Ref. 2; BAC29163)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 436 AA; 47181 MW; F54665575032B830 CRC64;
MGVRAAPSCA AAPAAAGAEQ SRRPGLWPPS PPPPLLLLLL LSLGLLHAGD CQQPTQCRIQ
KCTTDFVALT AHLNSAADGF DSEFCKALRA YAGCTQRTSK ACRGNLVYHS AVLGISDLMS
QRNCSKDGPT SSTNPEVTHD PCNYHSHGGV REHGGGDQRP PNYLFCGLFG DPHLRTFKDH
FQTCKVEGAW PLIDNNYLSV QVTNVPVVPG SSATATNKVT IIFKAQHECT DQKVYQAVTD
DLPAAFVDGT TSGGDGDVKS LHIVEKESGR YVEMHARYIG TTVFVRQLGR YLTLAIRMPE
DLAMSYEESQ DLQLCVNGCP MSECIDDGQG QVSAILGHSL PHTTSVQAWP GYTLETASTQ
CHEKMPVKDI YFQSCVFDLL TTGDANFTAA AHSALEDVEA LHPRKERWHI FPSSCGGCRD
LPVGLGLTCL ILIMFL
