RGPS1_DANRE
ID RGPS1_DANRE Reviewed; 581 AA.
AC B0UXH6; A6H8S8;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Ras-specific guanine nucleotide-releasing factor RalGPS1;
DE AltName: Full=Ral GEF with PH domain and SH3-binding motif 1;
DE AltName: Full=RalA exchange factor RalGPS1;
GN Name=ralgps1; ORFNames=si:dkey-191c17.1, zgc:165535;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Guanine nucleotide exchange factor. May be involved in
CC cytoskeletal organization. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cell membrane
CC {ECO:0000250}. Note=Associated with membranes through the PH domain.
CC {ECO:0000250}.
CC -!- DOMAIN: The PH domain mediates binding to membranes. {ECO:0000250}.
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DR EMBL; CT573394; CAQ14410.1; -; Genomic_DNA.
DR EMBL; CR361562; CAQ14410.1; JOINED; Genomic_DNA.
DR EMBL; CR361562; CAQ14617.1; -; Genomic_DNA.
DR EMBL; CT573394; CAQ14617.1; JOINED; Genomic_DNA.
DR EMBL; BC146736; AAI46737.1; -; mRNA.
DR RefSeq; NP_001093616.1; NM_001100146.1.
DR AlphaFoldDB; B0UXH6; -.
DR SMR; B0UXH6; -.
DR STRING; 7955.ENSDARP00000089610; -.
DR PaxDb; B0UXH6; -.
DR Ensembl; ENSDART00000098840; ENSDARP00000089610; ENSDARG00000068370.
DR GeneID; 100101642; -.
DR KEGG; dre:100101642; -.
DR CTD; 9649; -.
DR ZFIN; ZDB-GENE-070720-16; ralgps1.
DR eggNOG; KOG3417; Eukaryota.
DR GeneTree; ENSGT00940000154079; -.
DR HOGENOM; CLU_021333_0_1_1; -.
DR InParanoid; B0UXH6; -.
DR OMA; GPCICAL; -.
DR OrthoDB; 343666at2759; -.
DR PhylomeDB; B0UXH6; -.
DR TreeFam; TF352150; -.
DR PRO; PR:B0UXH6; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 8.
DR Bgee; ENSDARG00000068370; Expressed in retina and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR CDD; cd00155; RasGEF; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00617; RasGEF; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00147; RasGEF; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cytoplasm; Guanine-nucleotide releasing factor; Membrane;
KW Reference proteome.
FT CHAIN 1..581
FT /note="Ras-specific guanine nucleotide-releasing factor
FT RalGPS1"
FT /id="PRO_0000333195"
FT DOMAIN 49..288
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT DOMAIN 455..567
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 320..339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 329..332
FT /note="PXXP"
FT COMPBIAS 8..31
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 4
FT /note="R -> G (in Ref. 2; AAI46737)"
FT /evidence="ECO:0000305"
FT CONFLICT 428
FT /note="I -> M (in Ref. 2; AAI46737)"
FT /evidence="ECO:0000305"
FT CONFLICT 481
FT /note="R -> C (in Ref. 2; AAI46737)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 581 AA; 66038 MW; 98A3B306F7C7BADC CRC64;
MYRRNGLPAS VSITSRNTQD SSSSESLDGR SLDSAKSFDA VVFDMLKVTP EEFASQITLM
DAPVFKAIQP EELASCGWNK KEKHSLSPNV VAFTRRFNQV SFWAVREILT AQTLKIRAEI
LGHFIKIAKK LLELNNLHSL VSVVSALQSA PIFRLSKTWA LISRKDKATF EKLDFLTSKE
ENYNRMREYT RSLKMAPCIP YLGIYLFDMT YIDSAYPASD SIIETEQRTN QMNNLLRIIS
DLQVSCKYDH LITLPHVQKY LMSVRYIEEL QKFVEDDNYS LSLKIEPGNS SPRLVSSKED
LGGPSEVSMS VRYNRRPTCP DTSVVAHLPT PPPARHRKSH SLGNNMMCQF GVVESKSATF
PEKEKARHLL DDSFLESHSP VRNHTHDSVF TNGISLGSRE SSFSDELSST VERGRMYATL
GPNWRVPICN SPRSRSYIYS TPGAVSSYEC STLSSITIEG PLRRKTLMKE GKKPTLSSWK
RYWIVLSGSI LIYFGSKALR ANERRHYKSR PCKKITLTGW MVVLPDNPEH PNIFQLTDPD
RGNVYKFQTG SRFSAIIWHR HLAEACRSTR PQMPANLMSF E
