RGS20_CHICK
ID RGS20_CHICK Reviewed; 218 AA.
AC Q9PWA1;
DT 14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Regulator of G-protein signaling 20;
DE Short=RGS20;
DE AltName: Full=Gz-selective GTPase-activating protein;
DE Short=G(z)GAP;
DE Short=Gz-GAP;
GN Name=RGS20;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Spinal ganglion;
RX PubMed=10419452; DOI=10.1074/jbc.274.31.21507;
RA Jordan J.D., Carey K.D., Stork P.J.S., Iyengar R.;
RT "Modulation of rap activity by direct interaction of Galpha(o) with Rap1
RT GTPase-activating protein.";
RL J. Biol. Chem. 274:21507-21510(1999).
CC -!- FUNCTION: Inhibits signal transduction by increasing the GTPase
CC activity of G protein alpha subunits thereby driving them into their
CC inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-
CC i2 subunits, accelerates their GTPase activity and regulates their
CC signaling activities. The G(z)-alpha activity is inhibited by the
CC phosphorylation and palmitoylation of the G-protein. Negatively
CC regulates mu-opioid receptor-mediated activation of the G-proteins (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Forms a complex with G(alpha)z/i2 subunits and mu-opioid
CC receptors; the formation of this complex results in mu-opioid receptor
CC desensitization. Interacts with OPRM1 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Lipid-anchor. Nucleus. Cytoplasm.
CC Note=Shuttles between the cytoplasm/cell membrane and the nucleus.
CC Anchored to the membrane through palmitoylation. {ECO:0000250}.
CC -!- PTM: Fatty acylated. Heavily palmitoylated in the cysteine string motif
CC (By similarity). {ECO:0000250}.
CC -!- PTM: N- and O-glycosylated in synapsomal membranes. {ECO:0000250}.
CC -!- PTM: Sumoylated by SUMO1 and SUM02 in synaptosomes. The sumoylated
CC forms act as a scaffold for sequestering mu-opioid receptor-activated
CC G(alpha) subunits (By similarity). {ECO:0000250}.
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DR EMBL; AF151967; AAD45947.1; -; mRNA.
DR RefSeq; NP_990173.1; NM_204842.2.
DR AlphaFoldDB; Q9PWA1; -.
DR SMR; Q9PWA1; -.
DR STRING; 9031.ENSGALP00000041794; -.
DR PaxDb; Q9PWA1; -.
DR Ensembl; ENSGALT00000051502; ENSGALP00000044202; ENSGALG00000025941.
DR GeneID; 395646; -.
DR KEGG; gga:395646; -.
DR CTD; 8601; -.
DR VEuPathDB; HostDB:geneid_395646; -.
DR eggNOG; KOG3589; Eukaryota.
DR GeneTree; ENSGT00940000159123; -.
DR HOGENOM; CLU_059863_0_2_1; -.
DR InParanoid; Q9PWA1; -.
DR OMA; HQTMENE; -.
DR PhylomeDB; Q9PWA1; -.
DR PRO; PR:Q9PWA1; -.
DR Proteomes; UP000000539; Chromosome 2.
DR Bgee; ENSGALG00000025941; Expressed in brain and 7 other tissues.
DR ExpressionAtlas; Q9PWA1; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0009968; P:negative regulation of signal transduction; IEA:UniProtKB-KW.
DR Gene3D; 1.10.167.10; -; 1.
DR InterPro; IPR016137; RGS.
DR InterPro; IPR036305; RGS_sf.
DR InterPro; IPR044926; RGS_subdomain_2.
DR Pfam; PF00615; RGS; 1.
DR PRINTS; PR01301; RGSPROTEIN.
DR SMART; SM00315; RGS; 1.
DR SUPFAM; SSF48097; SSF48097; 1.
DR PROSITE; PS50132; RGS; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Glycoprotein; Lipoprotein; Membrane; Nucleus; Palmitate;
KW Reference proteome; Signal transduction inhibitor; Ubl conjugation.
FT CHAIN 1..218
FT /note="Regulator of G-protein signaling 20"
FT /id="PRO_0000204235"
FT DOMAIN 92..208
FT /note="RGS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00171"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 218 AA; 25141 MW; 188564BAA5868A38 CRC64;
MGSERTEMRK RQMAATQETP GTAQAQHSVG NRGPNACCFC WCCCCSCSCL TVRNQEEERA
RRTSHELQAE GIPNCEESPA PTLEEVNAWA QSFDKLMLTP AGRNAFREFL RTEFSEENML
FWMACEELKQ ESNKSVIEEK ARLIYEDYIS ILSPKEVSLD SRVREVINRN MLEPSQHTFD
DAQLQIYTLM HRDSYPRFMN SAIYKDLLRS LSEKSIEA
