RGS22_HUMAN
ID RGS22_HUMAN Reviewed; 1264 AA.
AC Q8NE09; A8K944; Q569L2; Q86Y71; Q9BYZ4; Q9UFN6;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Regulator of G-protein signaling 22;
DE Short=RGS22;
GN Name=RGS22;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), INTERACTION WITH GNA11; GNA12 AND
RP GNA13, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=18703424; DOI=10.1095/biolreprod.107.067504;
RA Hu Y., Xing J., Chen L., Guo X., Du Y., Zhao C., Zhu Y., Lin M., Zhou Z.,
RA Sha J.;
RT "RGS22, a novel testis-specific regulator of G-protein signaling involved
RT in human and mouse spermiogenesis along with GNA12/13 subunits.";
RL Biol. Reprod. 79:1021-1029(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 995-1264 (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
CC -!- FUNCTION: Inhibits signal transduction by increasing the GTPase
CC activity of G protein alpha subunits thereby driving them into their
CC inactive GDP-bound form. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with GNA11, GNA12 AND GNA13.
CC {ECO:0000269|PubMed:18703424}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18703424}. Nucleus
CC {ECO:0000269|PubMed:18703424}. Note=Expressed in the cytoplasm of
CC spermatogonia and spermatocytes. In spermatids, also expressed in the
CC nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8NE09-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8NE09-2; Sequence=VSP_022306;
CC Name=3;
CC IsoId=Q8NE09-3; Sequence=VSP_054225;
CC -!- TISSUE SPECIFICITY: Testis-specific. Expressed in Leydig cells and
CC spermatogenic cells from the spermatogonia to spermatid stages (at
CC protein level). {ECO:0000269|PubMed:18703424}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 3]: May be due to competing donor splice site.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG49397.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AY009106; AAG49397.1; ALT_FRAME; mRNA.
DR EMBL; AK292559; BAF85248.1; -; mRNA.
DR EMBL; AC021590; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP005356; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471060; EAW91794.1; -; Genomic_DNA.
DR EMBL; BC036665; AAH36665.2; -; mRNA.
DR EMBL; BC047060; AAH47060.1; -; mRNA.
DR EMBL; BC092411; AAH92411.1; -; mRNA.
DR EMBL; AL117544; CAB55986.2; -; mRNA.
DR CCDS; CCDS43758.1; -. [Q8NE09-1]
DR CCDS; CCDS69521.1; -. [Q8NE09-3]
DR PIR; T17296; T17296.
DR RefSeq; NP_001273621.1; NM_001286692.1. [Q8NE09-3]
DR RefSeq; NP_056483.3; NM_015668.4. [Q8NE09-1]
DR AlphaFoldDB; Q8NE09; -.
DR SMR; Q8NE09; -.
DR BioGRID; 117592; 4.
DR IntAct; Q8NE09; 1.
DR STRING; 9606.ENSP00000354109; -.
DR iPTMnet; Q8NE09; -.
DR PhosphoSitePlus; Q8NE09; -.
DR BioMuta; RGS22; -.
DR DMDM; 122064954; -.
DR EPD; Q8NE09; -.
DR jPOST; Q8NE09; -.
DR MassIVE; Q8NE09; -.
DR MaxQB; Q8NE09; -.
DR PaxDb; Q8NE09; -.
DR PeptideAtlas; Q8NE09; -.
DR PRIDE; Q8NE09; -.
DR ProteomicsDB; 1891; -.
DR ProteomicsDB; 73111; -. [Q8NE09-1]
DR ProteomicsDB; 73112; -. [Q8NE09-2]
DR Antibodypedia; 6845; 164 antibodies from 28 providers.
DR DNASU; 26166; -.
DR Ensembl; ENST00000360863.11; ENSP00000354109.6; ENSG00000132554.21. [Q8NE09-1]
DR Ensembl; ENST00000519725.5; ENSP00000427798.1; ENSG00000132554.21. [Q8NE09-2]
DR Ensembl; ENST00000523437.5; ENSP00000428212.1; ENSG00000132554.21. [Q8NE09-3]
DR GeneID; 26166; -.
DR KEGG; hsa:26166; -.
DR MANE-Select; ENST00000360863.11; ENSP00000354109.6; NM_015668.5; NP_056483.3.
DR UCSC; uc003yjb.2; human. [Q8NE09-1]
DR CTD; 26166; -.
DR DisGeNET; 26166; -.
DR GeneCards; RGS22; -.
DR HGNC; HGNC:24499; RGS22.
DR HPA; ENSG00000132554; Tissue enriched (testis).
DR MIM; 615650; gene.
DR neXtProt; NX_Q8NE09; -.
DR OpenTargets; ENSG00000132554; -.
DR PharmGKB; PA142671068; -.
DR VEuPathDB; HostDB:ENSG00000132554; -.
DR eggNOG; ENOG502QU18; Eukaryota.
DR GeneTree; ENSGT00500000044936; -.
DR HOGENOM; CLU_005031_0_0_1; -.
DR InParanoid; Q8NE09; -.
DR OMA; WAPRFWT; -.
DR OrthoDB; 240381at2759; -.
DR PhylomeDB; Q8NE09; -.
DR TreeFam; TF329128; -.
DR PathwayCommons; Q8NE09; -.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR SignaLink; Q8NE09; -.
DR BioGRID-ORCS; 26166; 9 hits in 1062 CRISPR screens.
DR ChiTaRS; RGS22; human.
DR GenomeRNAi; 26166; -.
DR Pharos; Q8NE09; Tbio.
DR PRO; PR:Q8NE09; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q8NE09; protein.
DR Bgee; ENSG00000132554; Expressed in sperm and 116 other tissues.
DR ExpressionAtlas; Q8NE09; baseline and differential.
DR Genevisible; Q8NE09; HS.
DR GO; GO:0015629; C:actin cytoskeleton; IDA:HPA.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0001650; C:fibrillar center; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0001965; F:G-protein alpha-subunit binding; IPI:UniProtKB.
DR GO; GO:0009968; P:negative regulation of signal transduction; IEA:UniProtKB-KW.
DR Gene3D; 1.10.167.10; -; 3.
DR InterPro; IPR016137; RGS.
DR InterPro; IPR042651; Rgs22.
DR InterPro; IPR036305; RGS_sf.
DR InterPro; IPR044926; RGS_subdomain_2.
DR PANTHER; PTHR46583; PTHR46583; 1.
DR Pfam; PF00615; RGS; 2.
DR SMART; SM00315; RGS; 2.
DR SUPFAM; SSF48097; SSF48097; 4.
DR PROSITE; PS50132; RGS; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Nucleus; Reference proteome;
KW Repeat; Signal transduction inhibitor.
FT CHAIN 1..1264
FT /note="Regulator of G-protein signaling 22"
FT /id="PRO_0000271376"
FT DOMAIN 852..980
FT /note="RGS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00171"
FT DOMAIN 1021..1145
FT /note="RGS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00171"
FT REGION 565..587
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1142..1174
FT /evidence="ECO:0000255"
FT COMPBIAS 565..584
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 187..198
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:18703424"
FT /id="VSP_054225"
FT VAR_SEQ 505..1264
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_022306"
FT VARIANT 397
FT /note="R -> M (in dbSNP:rs2446927)"
FT /id="VAR_051797"
FT VARIANT 943
FT /note="H -> Y (in dbSNP:rs3133711)"
FT /id="VAR_051798"
FT CONFLICT 86
FT /note="Missing (in Ref. 5; AAH92411)"
FT /evidence="ECO:0000305"
FT CONFLICT 131
FT /note="A -> P (in Ref. 5; AAH47060)"
FT /evidence="ECO:0000305"
FT CONFLICT 167
FT /note="P -> S (in Ref. 1; AAG49397)"
FT /evidence="ECO:0000305"
FT CONFLICT 378
FT /note="E -> K (in Ref. 5; AAH36665)"
FT /evidence="ECO:0000305"
FT CONFLICT 423
FT /note="K -> R (in Ref. 1; AAG49397)"
FT /evidence="ECO:0000305"
FT CONFLICT 692
FT /note="N -> D (in Ref. 1; AAG49397)"
FT /evidence="ECO:0000305"
FT CONFLICT 995..997
FT /note="LQK -> DAW (in Ref. 6; CAB55986)"
FT /evidence="ECO:0000305"
FT CONFLICT 1070
FT /note="S -> T (in Ref. 1; AAG49397)"
FT /evidence="ECO:0000305"
FT CONFLICT 1233
FT /note="K -> KK (in Ref. 6; CAB55986)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1264 AA; 147163 MW; 8647E53FE14B9D5D CRC64;
MPEKRLTAEP PTITEEEFED SLATDDFLVD YFNEFLSLPT FSEAIRFNAD YGVFEVANDA
PQFLEKQLKK ILQNQQPRNP IYDVVRKGKN EVKPVQMNAP DEDETINVNY NIMCLSREEG
IKWIKKERLP AFLESDCYFE YRLAKLVSQV RWSKSGMNFT VGSNFSPWIV KKPPSLPPPA
TEEDNLVIMK KFYVSLGEAS YTQTKDWFAL AKQSQQTVST FSLPCCVPYN KLKSPAISSV
SENFIFDDGV HPRTKKDPSK TNKLISEFEE EEGEEEEVSV SLQDTPSQAL LRVYLEKKQD
VDESLTMHFS TCEEFLSSYI YFILRGAIQQ IVGKPVGETP DYINFNNITK VSFDDCFESI
HGKNFLSELV QTTKERSEEI EQTSLSSKNE SAGPESRADW CISHRTYDIG NRKEFERFKK
FIKGTLGERY WWLWMDIERL KVLKDPGRHQ RHLEKMKKCY LVSNGDYYLS AEILSKFKLL
DGSQWNEEHL RNIQSEVLKP LLLYWAPRFC VTHSASTKYA SAELKFWHLR QAKPRKDIDP
FPQMATLLPL RPKSCIPQIP EIQKEEFSLS QPPKSPNKSP EVKTATQKPW KRELLYPGSS
KDDVIEKGSK YMSESSKVIH LTSFTDISEC LKPQLDRRYA YTEEPRVKTV SDVGALGGSD
MENLLQSLYV ENRAGFFFTK FCEHSGNKLW KNSVYFWFDL QAYHQLFYQE TLQPFKVCKQ
AQYLFATYVA PSATLDIGLQ QEKKKEIYMK IQPPFEDLFD TAEEYILLLL LEPWTKMVKS
DQIAYKKVEL VEETRQLDST YFRKLQALHK ETFSKKAEDT TCEIGTGILS LSNVSKRTEY
WDNVPAEYKH FKFSDLLNNK LEFEHFRQFL ETHSSSMDLM CWTDIEQFRR ITYRDRNQRK
AKSIYIKNKY LNKKYFFGPN SPASLYQQNQ VMHLSGGWGK ILHEQLDAPV LVEIQKHVQN
RLENVWLPLF LASEQFAARQ KIKVQMKDIA EELLLQKAEK KIGVWKPVES KWISSSCKII
AFRKALLNPV TSRQFQRFVA LKGDLLENGL LFWQEVQKYK DLCHSHCDES VIQKKITTII
NCFINSSIPP ALQIDIPVEQ AQKIIEHRKE LGPYVFREAQ MTIFGVLFKF WPQFCEFRKN
LTDENIMSVL ERRQEYNKQK KKLAVLEDEK SGKDGIKQYA NTSVPAIKTA LLSDSFLGLQ
PYGRQPTWCY SKYIEALEQE RILLKIQEEL EKKLFAGLQP LTNFKASSST MSLKKNMSAH
SSQK
