RGS22_MOUSE
ID RGS22_MOUSE Reviewed; 1258 AA.
AC G3UYX5;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Regulator of G-protein signaling 22;
GN Name=Rgs22;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=18703424; DOI=10.1095/biolreprod.107.067504;
RA Hu Y., Xing J., Chen L., Guo X., Du Y., Zhao C., Zhu Y., Lin M., Zhou Z.,
RA Sha J.;
RT "RGS22, a novel testis-specific regulator of G-protein signaling involved
RT in human and mouse spermiogenesis along with GNA12/13 subunits.";
RL Biol. Reprod. 79:1021-1029(2008).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Inhibits signal transduction by increasing the GTPase
CC activity of G protein alpha subunits thereby driving them into their
CC inactive GDP-bound form. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with GNA11, GNA12 AND GNA13. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18703424}. Nucleus
CC {ECO:0000269|PubMed:18703424}. Note=Expressed in the cytoplasm of
CC spermatogonia and spermatocytes. In spermatids, also expressed in the
CC nucleus.
CC -!- TISSUE SPECIFICITY: Expressed testis, including in Leydig cells and
CC spermatogenic cells from the spermatogonia to spermatid stages (at
CC protein level). {ECO:0000269|PubMed:18703424}.
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DR EMBL; AC121577; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC157521; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC166820; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS56979.1; -.
DR RefSeq; NP_001182677.1; NM_001195748.1.
DR RefSeq; XP_011243672.1; XM_011245370.2.
DR RefSeq; XP_011243673.1; XM_011245371.2.
DR RefSeq; XP_011243674.1; XM_011245372.2.
DR RefSeq; XP_011243675.1; XM_011245373.2.
DR AlphaFoldDB; G3UYX5; -.
DR SMR; G3UYX5; -.
DR BioGRID; 552756; 2.
DR STRING; 10090.ENSMUSP00000134259; -.
DR iPTMnet; G3UYX5; -.
DR PhosphoSitePlus; G3UYX5; -.
DR EPD; G3UYX5; -.
DR MaxQB; G3UYX5; -.
DR PaxDb; G3UYX5; -.
DR PRIDE; G3UYX5; -.
DR ProteomicsDB; 255193; -.
DR Antibodypedia; 6845; 164 antibodies from 28 providers.
DR Ensembl; ENSMUST00000172831; ENSMUSP00000134259; ENSMUSG00000037627.
DR GeneID; 626596; -.
DR KEGG; mmu:626596; -.
DR UCSC; uc011zsd.1; mouse.
DR CTD; 26166; -.
DR MGI; MGI:3613651; Rgs22.
DR VEuPathDB; HostDB:ENSMUSG00000037627; -.
DR eggNOG; ENOG502QU18; Eukaryota.
DR GeneTree; ENSGT00500000044936; -.
DR HOGENOM; CLU_005031_0_0_1; -.
DR InParanoid; G3UYX5; -.
DR OMA; WAPRFWT; -.
DR OrthoDB; 240381at2759; -.
DR PhylomeDB; G3UYX5; -.
DR TreeFam; TF329128; -.
DR BioGRID-ORCS; 626596; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Rgs22; mouse.
DR PRO; PR:G3UYX5; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; G3UYX5; protein.
DR Bgee; ENSMUSG00000037627; Expressed in spermatid and 40 other tissues.
DR ExpressionAtlas; G3UYX5; baseline and differential.
DR GO; GO:0015629; C:actin cytoskeleton; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0001650; C:fibrillar center; ISO:MGI.
DR GO; GO:0001673; C:male germ cell nucleus; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0001965; F:G-protein alpha-subunit binding; ISO:MGI.
DR GO; GO:0009968; P:negative regulation of signal transduction; IEA:UniProtKB-KW.
DR Gene3D; 1.10.167.10; -; 4.
DR InterPro; IPR016137; RGS.
DR InterPro; IPR042651; Rgs22.
DR InterPro; IPR036305; RGS_sf.
DR InterPro; IPR044926; RGS_subdomain_2.
DR PANTHER; PTHR46583; PTHR46583; 1.
DR Pfam; PF00615; RGS; 2.
DR SMART; SM00315; RGS; 2.
DR SUPFAM; SSF48097; SSF48097; 4.
DR PROSITE; PS50132; RGS; 2.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Nucleus; Reference proteome; Repeat;
KW Signal transduction inhibitor.
FT CHAIN 1..1258
FT /note="Regulator of G-protein signaling 22"
FT /id="PRO_0000428676"
FT DOMAIN 845..973
FT /note="RGS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00171"
FT DOMAIN 1014..1138
FT /note="RGS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00171"
FT REGION 581..604
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1145..1172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1145..1166
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1258 AA; 145883 MW; E3C90E88FE6E0901 CRC64;
MPEKRLSAEP PEVTEEEFEN YLATDNFLVD YFNEFLSLPT FPEAIRFNVD YGVFEVVNDA
PQLLEKQLKK ILQNQQPRNP IYDVVRKGKS DSKSTQKSVP CEDEAINVNY SIMCLKREQG
INWIKRERLP AFLESDCYFE YRLAKLISQA TWSSTGMNFI VGTNFTPWIL RRPPAPPPPS
TDEDNYMIMK KFYVSLGQAS YTQTKDWFTL AKESENTVTM ASLPCCIPHH QTASPVIATA
SEIFDDGVHP RTIKSLSKSS KAVSELDEEE EGSISMKDSP SQALLRVYLE KKGGKERNLT
LHFSSVEEFL DAYIIFILRE AIQHITGQSL SDTPEYINYY KVSHVIFDKV PPVPSNKAIV
SPPVEMVEEI SKDRLENVSL SSESESIGPE SRADWCISHR TYDIGNRREF ERFKKFLKGT
LGERYWWLWM DIERLKVLKD PERHQRHLEK MKKCYLVSSG ERYLSAEILS KFKLLHGSRW
TADHLKNIQA EVLKPLLLYW APRFCVTHSA SAKNASTELK FWRLRQEKPR KDVDPFPQMA
TLLPLRPKSC IPQTPEVQGE EINLFQPSKF KKLSKINAGT QQLGRSEPLN AVSSKDGGLE
KGSKRLPEST TVVRLTSFTD ISECLKPQLE RKYTYTEEHN VKTVSNVGAL GGFDMENLLQ
SLYVENRAGF FFTKFCENSG NKLWKHSVYF WFDLQAYHQL FYQETLQPFK VCKQAQYLFA
TYIAPSASFD IGLHQEGKKD IYMKIQPPFE DLFDTAEEFI LLSLLEPWTQ MVMSDKMAYK
KVELQEETRQ LDSACFRKLH ALHKETISKK AEDTTGYAMA KLSLSDVSKQ TEYWLNVPEG
YKHFTFTDLL NNKLEFEHFR QFLESHSSSL DLMCWIDIEQ FRRIIFKDQK QREEKSIYIK
NKYLNKKYFF GPRSPASLHQ QNQIMLLSGG WGRILHEQLD ASVLVEIQKH VLNRLENVWL
PLFLSSEQFA SRQKIKTQMK DIADELLLQR HDRKIGVWKP VESKWISSSC EIIAFRKALL
NPVIARQFQR FVALKGDLLE NGVLFWQEVQ KFKDLCHSHC DESIIHKKIT TIINCFINSY
IPPALQIDIP VEQAQKILEH RKELGPYVFR EAQMTIFGVL FKFWPQFCEF RKNLTDEKIM
SVLERRQEHK QKRKASDTEE DKAGKSGVKQ YASTTGSLTK PVLGSESLLV LQSYGRQPTW
CYSKYIEALE QERILLKIQE EVERKMFTGT SSFTNLLKPS TGSALSLKKN VSLHSIQR
