RGS2_RAT
ID RGS2_RAT Reviewed; 211 AA.
AC Q9JHX0;
DT 11-JUL-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Regulator of G-protein signaling 2;
DE Short=RGS2;
GN Name=Rgs2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Thoracic aorta;
RX PubMed=10692485; DOI=10.1124/mol.57.3.460;
RA Grant S.L., Lassegue B., Griendling K.K., Ushio-Fukai M., Lyons P.R.,
RA Alexander R.W.;
RT "Specific regulation of RGS2 messenger RNA by angiotensin II in cultured
RT vascular smooth muscle cells.";
RL Mol. Pharmacol. 57:460-467(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Wistar; TISSUE=Brain;
RA Taymans J.-M., Wintmolders C., Te Riele P., Jurzak M., Groenewegen H.J.,
RA Leysen J.E., Langlois X.;
RT "A detailed distribution study of RGS2 messenger RNA and protein in the rat
RT brain.";
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Ko J.K., Kim I.S., Park D.H.;
RT "PTH induced RGS cDNA sequence in rat osteoblast-like UMR106 cell.";
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-100.
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RX PubMed=11409749; DOI=10.1097/00001756-200106130-00043;
RA Robinet E.A., Wurch T., Pauwels P.J.;
RT "Different regulation of RGS2 mRNA by haloperidol and clozapine.";
RL NeuroReport 12:1731-1735(2001).
CC -!- FUNCTION: Regulates G protein-coupled receptor signaling cascades.
CC Inhibits signal transduction by increasing the GTPase activity of G
CC protein alpha subunits, thereby driving them into their inactive GDP-
CC bound form (By similarity). It is involved in the negative regulation
CC of the angiotensin-activated signaling pathway (By similarity). Plays a
CC role in the regulation of blood pressure in response to signaling via G
CC protein-coupled receptors and GNAQ. Plays a role in regulating the
CC constriction and relaxation of vascular smooth muscle (By similarity).
CC Binds EIF2B5 and blocks its activity, thereby inhibiting the
CC translation of mRNA into protein (By similarity).
CC {ECO:0000250|UniProtKB:O08849, ECO:0000250|UniProtKB:P41220}.
CC -!- SUBUNIT: Interacts with GNAQ. Does not interact with GNAI1 and GNAI3.
CC Interacts with EIF2B5. Interacts with PRKG1 (isoform alpha).
CC {ECO:0000250|UniProtKB:P41220}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P41220}.
CC Cytoplasm {ECO:0000250|UniProtKB:P41220}. Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:P41220}.
CC -!- PTM: Phosphorylated by protein kinase C. Phosphorylation by PRKG1 leads
CC to activation of RGS2 activity. {ECO:0000250|UniProtKB:P41220}.
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DR EMBL; AF279918; AAF85981.1; -; mRNA.
DR EMBL; AF321837; AAK09375.1; -; mRNA.
DR EMBL; AY043246; AAK85309.1; -; mRNA.
DR EMBL; BC061969; AAH61969.1; -; mRNA.
DR EMBL; AJ318489; CAC44900.1; -; mRNA.
DR RefSeq; NP_445905.1; NM_053453.2.
DR AlphaFoldDB; Q9JHX0; -.
DR SMR; Q9JHX0; -.
DR STRING; 10116.ENSRNOP00000005156; -.
DR PaxDb; Q9JHX0; -.
DR Ensembl; ENSRNOT00000005156; ENSRNOP00000005156; ENSRNOG00000003687.
DR GeneID; 84583; -.
DR KEGG; rno:84583; -.
DR UCSC; RGD:621665; rat.
DR CTD; 5997; -.
DR RGD; 621665; Rgs2.
DR eggNOG; KOG3589; Eukaryota.
DR GeneTree; ENSGT00940000157937; -.
DR HOGENOM; CLU_059863_3_2_1; -.
DR InParanoid; Q9JHX0; -.
DR OMA; GRMKRTI; -.
DR OrthoDB; 1246872at2759; -.
DR PhylomeDB; Q9JHX0; -.
DR TreeFam; TF315837; -.
DR Reactome; R-RNO-416476; G alpha (q) signalling events.
DR PRO; PR:Q9JHX0; -.
DR Proteomes; UP000002494; Chromosome 13.
DR Bgee; ENSRNOG00000003687; Expressed in lung and 20 other tissues.
DR Genevisible; Q9JHX0; RN.
DR GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR GO; GO:0009898; C:cytoplasmic side of plasma membrane; IDA:BHF-UCL.
DR GO; GO:0005829; C:cytosol; ISO:RGD.
DR GO; GO:0043005; C:neuron projection; IDA:RGD.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0010855; F:adenylate cyclase inhibitor activity; ISO:RGD.
DR GO; GO:0048487; F:beta-tubulin binding; IPI:RGD.
DR GO; GO:0001965; F:G-protein alpha-subunit binding; ISO:RGD.
DR GO; GO:0005096; F:GTPase activator activity; ISS:UniProtKB.
DR GO; GO:0007420; P:brain development; IEP:RGD.
DR GO; GO:0050873; P:brown fat cell differentiation; ISO:RGD.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0060135; P:maternal process involved in female pregnancy; IEP:RGD.
DR GO; GO:0140194; P:negative regulation of adenylate cyclase-inhibiting adrenergic receptor signaling pathway involved in heart process; ISO:RGD.
DR GO; GO:0010614; P:negative regulation of cardiac muscle hypertrophy; ISO:RGD.
DR GO; GO:0061052; P:negative regulation of cell growth involved in cardiac muscle cell development; IMP:RGD.
DR GO; GO:0045744; P:negative regulation of G protein-coupled receptor signaling pathway; IMP:RGD.
DR GO; GO:1900924; P:negative regulation of glycine import across plasma membrane; IMP:RGD.
DR GO; GO:0043407; P:negative regulation of MAP kinase activity; ISO:RGD.
DR GO; GO:0010519; P:negative regulation of phospholipase activity; ISO:RGD.
DR GO; GO:0017148; P:negative regulation of translation; IMP:RGD.
DR GO; GO:0030728; P:ovulation; IEP:RGD.
DR GO; GO:0060452; P:positive regulation of cardiac muscle contraction; ISO:RGD.
DR GO; GO:0010976; P:positive regulation of neuron projection development; IMP:RGD.
DR GO; GO:0008277; P:regulation of G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0055119; P:relaxation of cardiac muscle; ISO:RGD.
DR GO; GO:0060087; P:relaxation of vascular associated smooth muscle; ISO:RGD.
DR GO; GO:0001975; P:response to amphetamine; IEP:RGD.
DR GO; GO:0045471; P:response to ethanol; IEP:RGD.
DR GO; GO:0007283; P:spermatogenesis; ISO:RGD.
DR Gene3D; 1.10.167.10; -; 1.
DR Gene3D; 1.10.196.10; -; 1.
DR InterPro; IPR016137; RGS.
DR InterPro; IPR034947; RGS2.
DR InterPro; IPR036305; RGS_sf.
DR InterPro; IPR024066; RGS_subdom1/3.
DR InterPro; IPR044926; RGS_subdomain_2.
DR PANTHER; PTHR10845:SF43; PTHR10845:SF43; 1.
DR Pfam; PF00615; RGS; 1.
DR PRINTS; PR01301; RGSPROTEIN.
DR SMART; SM00315; RGS; 1.
DR SUPFAM; SSF48097; SSF48097; 1.
DR PROSITE; PS50132; RGS; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell membrane; Cytoplasm; GTPase activation; Membrane; Nucleus;
KW Phosphoprotein; Reference proteome; Signal transduction inhibitor;
KW Translation regulation.
FT CHAIN 1..211
FT /note="Regulator of G-protein signaling 2"
FT /id="PRO_0000204181"
FT DOMAIN 83..199
FT /note="RGS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00171"
FT REGION 32..66
FT /note="Necessary for membrane association"
FT /evidence="ECO:0000250|UniProtKB:P41220"
FT REGION 79..116
FT /note="Necessary to inhibit protein synthesis"
FT /evidence="ECO:0000250|UniProtKB:P41220"
SQ SEQUENCE 211 AA; 24323 MW; 84B5525CB41EA964 CRC64;
MQSAMFLAVQ HDCVPMDKSA GNGPKVEEKR EKMKRTLLKD WKTRLSYFLQ NSSTPGKPKT
GKKSKQQTFI KPSPEEALLW AEAFDELLAS KYGLAAFRAF LKSEFCEENI EFWLACEDFK
KTKSPQKLSS KARKIYTDFI EKEAPKEINI DFQTKTLIAQ NIQEATSGCF TTAQKRVYSL
MENNSYPRFL ESEFYQDLCK KPQITTEPHA T
