RGS9_BOVIN
ID RGS9_BOVIN Reviewed; 484 AA.
AC O46469;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 25-MAY-2022, entry version 158.
DE RecName: Full=Regulator of G-protein signaling 9;
DE Short=RGS9;
GN Name=RGS9;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9459445; DOI=10.1016/s0896-6273(00)80437-7;
RA He W., Cowan C.W., Wensel T.G.;
RT "RGS9, a GTPase accelerator for phototransduction.";
RL Neuron 20:95-102(1998).
RN [2]
RP INTERACTION WITH GNB5.
RX PubMed=10051575; DOI=10.1073/pnas.96.5.1947;
RA Makino E.R., Handy J.W., Li T., Arshavsky V.Y.;
RT "The GTPase activating factor for transducin in rod photoreceptors is the
RT complex between RGS9 and type 5 G protein beta subunit.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:1947-1952(1999).
RN [3]
RP INTERACTION WITH RGS9BP.
RX PubMed=12119397; DOI=10.1073/pnas.152094799;
RA Hu G., Wensel T.G.;
RT "R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein,
RT RGS9-1.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:9755-9760(2002).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS) OF 276-422 IN COMPLEX WITH PDE6G AND
RP GNAT1.
RX PubMed=11234020; DOI=10.1038/35059138;
RA Slep K.C., Kercher M.A., He W., Cowan C.W., Wensel T.G., Sigler P.B.;
RT "Structural determinants for regulation of phosphodiesterase by a G protein
RT at 2.0 A.";
RL Nature 409:1071-1077(2001).
CC -!- FUNCTION: Inhibits signal transduction by increasing the GTPase
CC activity of G protein alpha subunits thereby driving them into their
CC inactive GDP-bound form. Binds to GNAT1. Involved in phototransduction;
CC key element in the recovery phase of visual transduction.
CC -!- SUBUNIT: Heterodimer with GNB5. Interacts with RGS7BP, leading to
CC regulate the subcellular location of the heterodimer formed with GNB5.
CC Component of the RGS9-1-Gbeta5 complex composed of RGS9 (RGS9-1),
CC Gbeta5 (GNB5) and RGS9BP (Probable). Interacts with PDE6G and GNAT1
CC (PubMed:11234020). {ECO:0000269|PubMed:11234020,
CC ECO:0000305|PubMed:10051575, ECO:0000305|PubMed:12119397}.
CC -!- INTERACTION:
CC O46469; A5PJS1: GNB5; NbExp=5; IntAct=EBI-6949890, EBI-6949917;
CC -!- SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein.
CC Note=Targeted to the membrane via its interaction with RGS9BP.
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Photoreceptor outer segments.
CC -!- PTM: Phosphorylation is decreased by light exposition.
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DR EMBL; AF011357; AAC99480.1; -; mRNA.
DR RefSeq; NP_776595.1; NM_174170.2.
DR PDB; 1FQI; X-ray; 1.94 A; A=276-422.
DR PDB; 1FQJ; X-ray; 2.02 A; B/E=276-422.
DR PDB; 1FQK; X-ray; 2.30 A; B/D=276-422.
DR PDBsum; 1FQI; -.
DR PDBsum; 1FQJ; -.
DR PDBsum; 1FQK; -.
DR AlphaFoldDB; O46469; -.
DR SMR; O46469; -.
DR CORUM; O46469; -.
DR IntAct; O46469; 2.
DR MINT; O46469; -.
DR STRING; 9913.ENSBTAP00000023605; -.
DR iPTMnet; O46469; -.
DR PaxDb; O46469; -.
DR GeneID; 281453; -.
DR KEGG; bta:281453; -.
DR CTD; 8787; -.
DR eggNOG; KOG3589; Eukaryota.
DR InParanoid; O46469; -.
DR OrthoDB; 415625at2759; -.
DR EvolutionaryTrace; O46469; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0097381; C:photoreceptor disc membrane; TAS:Reactome.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IEA:InterPro.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0009968; P:negative regulation of signal transduction; IEA:UniProtKB-KW.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR CDD; cd00068; GGL; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.167.10; -; 1.
DR Gene3D; 4.10.260.10; -; 1.
DR InterPro; IPR000591; DEP_dom.
DR InterPro; IPR015898; G-protein_gamma-like_dom.
DR InterPro; IPR036284; GGL_sf.
DR InterPro; IPR016137; RGS.
DR InterPro; IPR040759; RGS_DHEX.
DR InterPro; IPR036305; RGS_sf.
DR InterPro; IPR044926; RGS_subdomain_2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00610; DEP; 1.
DR Pfam; PF00631; G-gamma; 1.
DR Pfam; PF00615; RGS; 1.
DR Pfam; PF18148; RGS_DHEX; 1.
DR PRINTS; PR01301; RGSPROTEIN.
DR SMART; SM00049; DEP; 1.
DR SMART; SM00224; GGL; 1.
DR SMART; SM00315; RGS; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF48097; SSF48097; 1.
DR SUPFAM; SSF48670; SSF48670; 1.
DR PROSITE; PS50186; DEP; 1.
DR PROSITE; PS50132; RGS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Membrane; Phosphoprotein; Reference proteome;
KW Sensory transduction; Signal transduction inhibitor; Vision.
FT CHAIN 1..484
FT /note="Regulator of G-protein signaling 9"
FT /id="PRO_0000204202"
FT DOMAIN 30..105
FT /note="DEP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00066"
FT DOMAIN 219..280
FT /note="G protein gamma"
FT DOMAIN 299..414
FT /note="RGS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00171"
FT REGION 460..484
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 282..284
FT /evidence="ECO:0007829|PDB:1FQJ"
FT HELIX 290..295
FT /evidence="ECO:0007829|PDB:1FQI"
FT TURN 296..298
FT /evidence="ECO:0007829|PDB:1FQI"
FT HELIX 300..304
FT /evidence="ECO:0007829|PDB:1FQI"
FT HELIX 307..319
FT /evidence="ECO:0007829|PDB:1FQI"
FT HELIX 324..336
FT /evidence="ECO:0007829|PDB:1FQI"
FT HELIX 340..342
FT /evidence="ECO:0007829|PDB:1FQI"
FT HELIX 343..354
FT /evidence="ECO:0007829|PDB:1FQI"
FT HELIX 367..376
FT /evidence="ECO:0007829|PDB:1FQI"
FT TURN 382..385
FT /evidence="ECO:0007829|PDB:1FQI"
FT HELIX 386..399
FT /evidence="ECO:0007829|PDB:1FQI"
FT HELIX 401..405
FT /evidence="ECO:0007829|PDB:1FQI"
FT HELIX 408..416
FT /evidence="ECO:0007829|PDB:1FQI"
SQ SEQUENCE 484 AA; 56680 MW; 3D8C61CEC1E22949 CRC64;
MTIRHQGQQY RPRMAFLRKI EALVKDMQDP DTGVRVQNQK VKVVSIPHAM TGSDVLQWIS
QRLWISGLEA QNLGNFIVKY GYIYPLQDPR NLTLKPDSSL YRFQTPYFWP TQQWPAEDVD
YAIYLAKRNI KKKGILEEYE KENYNFLNKK INYKWDFVIM QAREQYRAGK ERNKVDRCAL
DCQEKAYWLV HRCPPGANNV LDYGLDRVTN PNEDQKQTVV SVRKEIMYYR QALMRSTVKS
SVSLGGIVKY SEQFSSNDAI MSGCLPSNPW ITDDTQFWDL NAKLVDIPTK MRVERWAFNF
SELIRDPKGR QSFQHFLRKE FSGENLGFWE ACEDLKYGDQ SKVKEKAEEI YKLFLAPGAR
RWINIDGKTM DITVKGLKHP HRYVLDAAQT HIYMLMKKDS YARYLKSPIY KEMLAKAIEP
QGTTRKSSSL PFMRRHLRSS PSPVILRQLE EEAKAREAAT TVDITQVMSK LDRRSQLRKE
PPPK
