RH10_ORYSI
ID RH10_ORYSI Reviewed; 472 AA.
AC A2XKG2;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 10 {ECO:0000305};
DE EC=3.6.4.13 {ECO:0000269|PubMed:26848586};
DE AltName: Full=DEAD-box RNA helicase TOGR1 {ECO:0000303|PubMed:26848586};
DE AltName: Full=Protein THERMOTOLERANT GROWTH REQUIRED1 {ECO:0000303|PubMed:26848586};
GN Name=RH10 {ECO:0000305}; Synonyms=TOGR1 {ECO:0000303|PubMed:26848586};
GN ORFNames=OsI_12939 {ECO:0000312|EMBL:EAY91322.1};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946 {ECO:0000312|EMBL:EAY91322.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, SUBUNIT, SUBCELLULAR
RP LOCATION, DEVELOPMENTAL STAGE, INDUCTION, DISRUPTION PHENOTYPE, AND
RP MUTAGENESIS OF GLY-47.
RC STRAIN=cv. Zhongxian 3037 {ECO:0000303|PubMed:26848586};
RX PubMed=26848586; DOI=10.1371/journal.pgen.1005844;
RA Wang D., Qin B., Li X., Tang D., Zhang Y., Cheng Z., Xue Y.;
RT "Nucleolar DEAD-box RNA helicase TOGR1 regulates thermotolerant growth as a
RT pre-rRNA chaperone in rice.";
RL PLoS Genet. 12:E1005844-E1005844(2016).
CC -!- FUNCTION: Has ATP-dependent RNA helicase activity in vitro. Acts as a
CC thermosensitive RNA chaperone required for normal processing of pre-
CC rRNA intermediates. Required for normal cell division at high
CC temperatures. Required for a primary metabolism adaptation to high
CC temperatures to support thermotolerant growth by regulating gene
CC expression. Partially rescues the yeast rRNA helicase RRP3 mutant which
CC has repressed cell proliferation at 38 degrees Celsius.
CC {ECO:0000269|PubMed:26848586}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000269|PubMed:26848586};
CC -!- ACTIVITY REGULATION: Helicase activity is activated by temperature
CC increase. {ECO:0000269|PubMed:26848586}.
CC -!- SUBUNIT: Interacts with U3 small nucleolar RNA (U3 snoRNA) as part of
CC the ribosomal small subunit (SSU) processome.
CC {ECO:0000269|PubMed:26848586}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:26848586}. Nucleus,
CC nucleolus {ECO:0000269|PubMed:26848586}. Note=Localization is not
CC affected by temperature. {ECO:0000269|PubMed:26848586}.
CC -!- DEVELOPMENTAL STAGE: Widely expressed in roots, shoots, leaves, culms,
CC spikelets and anthers during different developing stages.
CC {ECO:0000269|PubMed:26848586}.
CC -!- INDUCTION: By high temperature and the circadian clock. Expression
CC peaks at zeitgeber time 9 (ZT9) corresponding to afternoon of a
CC subjective day, the hottest time of daily temperature fluctuation.
CC {ECO:0000269|PubMed:26848586}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Thermosensitive dwarf mutant. Reduced plant
CC height, root length and crown root number at 30, 32.5 and 35 degrees
CC Celsius, whereas they grow similarly to wild-type at 25 degrees Celsius
CC except for a slightly reduced plant height. At tillering stage, plants
CC grown in a hot climate show narrowed leaf blades mainly due to a
CC decrease in vascular bundle and lateral vein numbers, and all
CC internodes are shortened. Furthermore, cross-sections of leaf sheath,
CC leaf blades, internodes and root maturation zones do not show a
CC significant difference in cell elongation, but a reduction in cell
CC numbers compared to wild-type. {ECO:0000269|PubMed:26848586}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX47/RRP3
CC subfamily. {ECO:0000305}.
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DR EMBL; CM000128; EAY91322.1; -; Genomic_DNA.
DR AlphaFoldDB; A2XKG2; -.
DR SMR; A2XKG2; -.
DR STRING; 39946.A2XKG2; -.
DR EnsemblPlants; BGIOSGA010088-TA; BGIOSGA010088-PA; BGIOSGA010088.
DR Gramene; BGIOSGA010088-TA; BGIOSGA010088-PA; BGIOSGA010088.
DR HOGENOM; CLU_003041_1_1_1; -.
DR OMA; YDIELYQ; -.
DR Proteomes; UP000007015; Chromosome 3.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR CDD; cd17954; DEADc_DDX47; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR044765; DDX47/Rrp3_DEADc.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome; RNA-binding; rRNA processing; Stress response.
FT CHAIN 1..472
FT /note="DEAD-box ATP-dependent RNA helicase 10"
FT /id="PRO_0000443718"
FT DOMAIN 72..245
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 256..416
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 429..472
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 41..69
FT /note="Q motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00552"
FT MOTIF 193..196
FT /note="DEAD box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT COMPBIAS 429..461
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 85..92
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT MUTAGEN 47
FT /note="G->V: In togr1-1; thermosensitive dwarf mutant."
FT /evidence="ECO:0000269|PubMed:26848586"
SQ SEQUENCE 472 AA; 52480 MW; 019998F2508FB19B CRC64;
MAKKKDVEVE ELDEEVVAAA AAPAADGGEE QEAEPPARRP STFAELGVVP ELVAACDAMG
WKEPTRIQAE AIPHALEGRD LIGLGQTGSG KTGAFALPII QALLKQDKPQ ALFACVLSPT
RELAFQIGQQ FEALGSAIGL SCTVLVGGVD RVQQAVSLAK RPHIVVGTPG RLLDHLTDTK
GFSLNKLKYL VLDEADKLLN VEFQKALDDI LNVIPKERRT FLFSATMTNK VSKLQRACLR
NPVKVEVASK YSTVDTLRQE FYFVPADYKD CFLVHVLNEL PGSMIMIFVR TCESTRLLAL
TLRNLRFKAI SISGQMSQDK RLGALNRFKT KDCNILICTD VASRGLDIQG VDVVINYDIP
MNSKDYVHRV GRTARAGNTG YAVSLVNQYE AMWFKMIEKL LGYEIPDRKV DNAEIMILRE
RISDSKRIAL TTMKEGGGHK KKRRKNEDDE EEEERNAPVS RKSKSFNKSR RR
