ID   RH13_ORYSI              Reviewed;         832 AA.
AC   A2XVF7; Q01HE8; Q7XQ07;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase 13;
DE            EC=3.6.4.13;
GN   ORFNames=OsI_016050, OSIGBa0157K09-H0214G12.9;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Guang-Lu-Ai No.4;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC       family of RNA helicases and controls ATP binding and hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX24/MAK5
CC       subfamily. {ECO:0000305}.
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DR   EMBL; CR855236; CAH67998.1; -; Genomic_DNA.
DR   EMBL; CM000129; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; A2XVF7; -.
DR   SMR; A2XVF7; -.
DR   STRING; 39946.A2XVF7; -.
DR   iPTMnet; A2XVF7; -.
DR   Proteomes; UP000007015; Chromosome 4.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW   RNA-binding.
FT   CHAIN           1..832
FT                   /note="DEAD-box ATP-dependent RNA helicase 13"
FT                   /id="PRO_0000302058"
FT   DOMAIN          230..447
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          484..645
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   REGION          1..59
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          91..173
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          800..832
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           198..226
FT                   /note="Q motif"
FT   MOTIF           371..374
FT                   /note="DEAD box"
FT   COMPBIAS        1..17
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        802..816
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        817..832
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         243..250
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   CONFLICT        663
FT                   /note="K -> Y (in Ref. 2; CM000129)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        796
FT                   /note="Q -> L (in Ref. 2; CM000129)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   832 AA;  93309 MW;  78D7553345F03CBA CRC64;
     MAAAPPPPPP PQLQSSDPST PPQETSQVRK GKKSRGAKKP RRAAAAAAAS TSSAGTMVED
     PFLVLAGGKE GGFLELEEID EADFGIFGGA VEDLGEIDRK AGKDQKKKKR KKRKRGDDDY
     ALPGDGDLVV ECEEEGEKGE KRVKKKRRSR KKRKVKEMEE KMESKEDVSD DNVEDMQDGN
     DMEQDNNDGL ILGEDEVYAW RELRLHPLLI TAVRRLGFKE PTPIQKACFP AAAHQGKDVI
     GAAETGSGKT LAFGLPILQR LLEEQEKAMR LSREDESTQD ENSRESPLRA LILTPTRELA
     KQVCDHLKEA AKFLRIQVVP IVGGLSMEKQ ERLLKRKPEI VVGTPGRLWE LMSTGNQHLI
     KLHSLSFFVL DEADRMIERG HFHELQSIIE MLPVTNGSDE QTVGTTPSCE TVPILQIKKR
     QTFVFSATLA LSANFRKKLK RGLVTAKASA STDLSSIEAL SKQARMKPNA EIVDLTKASI
     LPEKLEESFI ECSDDDKDAY LYYILSVHGQ GRTIIFCTSI AALRHLSSIL RVLGINVLTN
     HAQMQQRARM KAVDRFRESE NSILVATDGF ARGMDFDDVR TVIHYQLPHS TDVYIHRSGR
     TARKSMAGCS IALISPADKA KFYSLCKSLS KENLQQFPVD HAYMPAVMNR LTLARQIDKI
     TRKNSQENAN KSWLQRNAES MGLLLETSDS EEERVQGHKQ RKATSANLQK LQQDLSELLQ
     RPLQPKTFSR RYLAGAGVSP LLQKQLEELS KRNVKGSASV NANKGSRFVV IGQDQIEPLQ
     ALQNSGQEVC VSIDKQREKR RLAENWRRKK QKEKKSTREQ KRKEKRIAKE RD