RH14_ORYSJ
ID RH14_ORYSJ Reviewed; 708 AA.
AC Q5VQL1; Q5VQL2;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 14;
DE EC=3.6.4.13;
GN OrderedLocusNames=Os01g0172200, LOC_Os01g07740;
GN ORFNames=P0583G08.30-1, P0583G08.30-2;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: ATP-dependent RNA helicase involved nonsense-mediated mRNA
CC decay and ribosome biogenesis through rRNA processing. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q5VQL1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5VQL1-2; Sequence=VSP_024155;
CC Name=3;
CC IsoId=Q5VQL1-3; Sequence=VSP_024156;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- MISCELLANEOUS: [Isoform 3]: May be due to competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX5/DBP2
CC subfamily. {ECO:0000305}.
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DR EMBL; AP003282; BAD68263.1; -; Genomic_DNA.
DR EMBL; AP003282; BAD68264.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF04065.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS70632.1; -; Genomic_DNA.
DR EMBL; AK100326; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015621419.1; XM_015765933.1. [Q5VQL1-2]
DR RefSeq; XP_015621421.1; XM_015765935.1. [Q5VQL1-1]
DR RefSeq; XP_015621422.1; XM_015765936.1. [Q5VQL1-3]
DR AlphaFoldDB; Q5VQL1; -.
DR SMR; Q5VQL1; -.
DR STRING; 4530.OS01T0172200-01; -.
DR PRIDE; Q5VQL1; -.
DR EnsemblPlants; Os01t0172200-01; Os01t0172200-01; Os01g0172200. [Q5VQL1-2]
DR GeneID; 4327645; -.
DR Gramene; Os01t0172200-01; Os01t0172200-01; Os01g0172200. [Q5VQL1-2]
DR KEGG; osa:4327645; -.
DR eggNOG; KOG0331; Eukaryota.
DR InParanoid; Q5VQL1; -.
DR OMA; VPFTEQN; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR CDD; cd00201; WW; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR InterPro; IPR001202; WW_dom.
DR InterPro; IPR036020; WW_dom_sf.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00456; WW; 1.
DR SUPFAM; SSF51045; SSF51045; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
DR PROSITE; PS50020; WW_DOMAIN_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Helicase; Hydrolase;
KW Nonsense-mediated mRNA decay; Nucleotide-binding; Nucleus;
KW Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing.
FT CHAIN 1..708
FT /note="DEAD-box ATP-dependent RNA helicase 14"
FT /id="PRO_0000282454"
FT DOMAIN 21..55
FT /note="WW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00224"
FT DOMAIN 211..385
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 414..558
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 48..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 560..708
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 180..208
FT /note="Q motif"
FT MOTIF 333..336
FT /note="DEAD box"
FT COMPBIAS 54..69
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 71..88
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 93..112
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 116..138
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 583..657
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 665..687
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 224..231
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT VAR_SEQ 195
FT /note="V -> VSAHNLHDYLMHFLVSFSKEIRRSLCVHTTYVITTLDVHLSRLFKST
FT IYVKV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_024155"
FT VAR_SEQ 650
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_024156"
SQ SEQUENCE 708 AA; 77297 MW; 64691FB4539E972E CRC64;
MASAAAAAAT ARGPRYAPPD PTLPKPWRGL IDGNTGYLYF WNPETKAVQY DRPTAPPPSS
PPAQQPPERP RNSDPAESQA QAGASRTQNA APADDRARND HLNDHFERRT EAAGSHAQNV
PFTEQNTRSN PSSQPCSAAG VYPAQNVFSE AASGDRTSPE AYRAKHEITI VGNEAPAPFM
TFQSTGFPPE ILREVQQAGF SAPTPIQAQS WPIALRNRDI VAVAKTGSGK TLGYLIPGFI
LLKRLQHNSR DGPTVLVLSP TRELATQIQD EAKKFGRSSR ISSVCLYGGA PKGPQLRDLE
RGADIVVATP GRLNDILEMR RVSLHQVSYL VLDEADRMLD MGFEPQIRKI VKQVQPKRQT
LMFTATWPKE VRKIASDLLS NPVQVNIGNT DQLVANKSIT QYVDVITPPE KSRRLDQILR
SQEPGSKIII FCSTKRMCDQ LARNLARQYG ASAIHGDKSQ AERDSVLSEF RSGRCPILVA
TDVAARGLDI KDIRVVVNYD FPTGVEDYVH RIGRTGRAGA TGVAYTFFCD QDSKYASDLV
KILEGANQSV SQQLRDMVSR GGYGGRSRRW ASSDDSYGGR GYDSGYTSRS TDNYNSGYGS
QSGNGSSFHS SFHNSNSGNQ FGDTSGFQTS FHNSSSNNQT SDNPSFHASS NNDQPGDGLS
FHARFYSSSR GSDQSRTNNA GFRDRSRSPP SNRNHEDPGS KAVGVSNW
