RH15_ORYSJ
ID RH15_ORYSJ Reviewed; 432 AA.
AC Q5JK84; B7F820;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 15;
DE EC=3.6.4.13;
DE AltName: Full=API5-interacting protein 1 {ECO:0000303|PubMed:21467577};
GN Name=AIP2 {ECO:0000303|PubMed:21467577};
GN OrderedLocusNames=Os01g0550000, LOC_Os01g36920;
GN ORFNames=B1156H12.21-1, OSJNBa0024F24.6-1;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION, SUBUNIT, INTERACTION WITH API5, AND SUBCELLULAR LOCATION.
RX PubMed=21467577; DOI=10.1105/tpc.110.082636;
RA Li X., Gao X., Wei Y., Deng L., Ouyang Y., Chen G., Li X., Zhang Q., Wu C.;
RT "Rice APOPTOSIS INHIBITOR5 coupled with two DEAD-box adenosine 5'-
RT triphosphate-dependent RNA helicases regulates tapetum degeneration.";
RL Plant Cell 23:1416-1434(2011).
CC -!- FUNCTION: ATP-binding RNA helicase involved in pre-mRNA splicing.
CC Required for the export of mRNA out of the nucleus (By similarity).
CC Required for tapetal programmed cell death (PCD) and degeneration
CC during anther development. Forms dimer with AIP1 and binds the promoter
CC region of the cysteine protease CP1. Can complement the yeast RNA
CC helicase SUB2. Plants silencing AIP1 and AIP2 are male sterile
CC (PubMed:21467577). {ECO:0000250|UniProtKB:Q07478,
CC ECO:0000269|PubMed:21467577}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBUNIT: Homodimer and heterodimer with AIP1 (PubMed:21467577).
CC Interacts with API5 (PubMed:21467577). {ECO:0000269|PubMed:21467577}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21467577}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DECD subfamily.
CC {ECO:0000305}.
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DR EMBL; AP004225; BAD88055.1; -; Genomic_DNA.
DR EMBL; AP004258; BAD88115.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF05212.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS72635.1; -; Genomic_DNA.
DR EMBL; AK122050; BAH00768.1; -; mRNA.
DR RefSeq; XP_015621815.1; XM_015766329.1.
DR AlphaFoldDB; Q5JK84; -.
DR SMR; Q5JK84; -.
DR STRING; 4530.OS01T0550000-01; -.
DR PaxDb; Q5JK84; -.
DR PRIDE; Q5JK84; -.
DR EnsemblPlants; Os01t0550000-01; Os01t0550000-01; Os01g0550000.
DR GeneID; 4326193; -.
DR Gramene; Os01t0550000-01; Os01t0550000-01; Os01g0550000.
DR KEGG; osa:4326193; -.
DR eggNOG; KOG0329; Eukaryota.
DR HOGENOM; CLU_003041_1_0_1; -.
DR InParanoid; Q5JK84; -.
DR OMA; INFELPM; -.
DR OrthoDB; 779000at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR ExpressionAtlas; Q5JK84; baseline and differential.
DR Genevisible; Q5JK84; OS.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR GO; GO:0048653; P:anther development; IMP:UniProtKB.
DR GO; GO:0009555; P:pollen development; IMP:UniProtKB.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Coiled coil; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome; RNA-binding.
FT CHAIN 1..432
FT /note="DEAD-box ATP-dependent RNA helicase 15"
FT /id="PRO_0000282496"
FT DOMAIN 82..255
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 283..428
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT COILED 3..28
FT /evidence="ECO:0000255"
FT MOTIF 51..79
FT /note="Q motif"
FT MOTIF 202..205
FT /note="DEAD box"
FT BINDING 95..102
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 432 AA; 48567 MW; EE00942821DC1F2F CRC64;
MGEAEVKDNE VYEEDLVDYE EEVENGADGG AAAANASADV VKKGYVGIHS SGFRDFLLKP
ELLRAIQDCG FEHPSEVQHE CIPQAILGMD VICQAKSGMG KTAVFVLSSL QQIDPVAGQV
GALVLCHTRE LAYQICHEFE RFSKYLPEVK VAVFYGGVHI KKHKDLLKND CPHIVVGTPG
RILALAREKD LSLKNVRHFI LDECDKMLDS LDMRRDVQEI FKMTPHDKQV MMFSATLSKE
IRPVCKKFMQ DPMEIYVDDE AKLTLHGLVQ HYIKLSEAEK NRKLNDLLDA LDFNQVVIFV
KSVSRAAELN KLLCECNFPA ISIHSGMTQE ERLTRYKNFK EGHKRILVAT DLVGRGIDIE
RVNIVINYDM PDSADSYLHR VGRAGRFGTK GLAITFVSSA SDSDVLNQVQ ERFEVDIKEL
PEQIDTSTYM PS
