RH18_ARATH
ID RH18_ARATH Reviewed; 593 AA.
AC Q9FLB0; Q9ZS03;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 18;
DE EC=3.6.4.13;
GN Name=RH18; OrderedLocusNames=At5g05450; ORFNames=K18I23.26;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 234-593.
RC STRAIN=cv. Columbia;
RX PubMed=9862990; DOI=10.1093/nar/27.2.628;
RA Aubourg S., Kreis M., Lecharny A.;
RT "The DEAD box RNA helicase family in Arabidopsis thaliana.";
RL Nucleic Acids Res. 27:628-636(1999).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17168887; DOI=10.1111/j.1467-7652.2004.00084.x;
RA Mingam A., Toffano-Nioche C., Brunaud V., Boudet N., Kreis M., Lecharny A.;
RT "DEAD-box RNA helicases in Arabidopsis thaliana: establishing a link
RT between quantitative expression, gene structure and evolution of a family
RT of genes.";
RL Plant Biotechnol. J. 2:401-415(2004).
RN [5]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX55/SPB4
CC subfamily. {ECO:0000305}.
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DR EMBL; AB010692; BAB09988.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90875.1; -; Genomic_DNA.
DR EMBL; AJ010469; CAA09208.1; -; mRNA.
DR PIR; T51344; T51344.
DR RefSeq; NP_196164.1; NM_120627.5.
DR AlphaFoldDB; Q9FLB0; -.
DR SMR; Q9FLB0; -.
DR STRING; 3702.AT5G05450.1; -.
DR PaxDb; Q9FLB0; -.
DR PRIDE; Q9FLB0; -.
DR ProteomicsDB; 236940; -.
DR EnsemblPlants; AT5G05450.1; AT5G05450.1; AT5G05450.
DR GeneID; 830428; -.
DR Gramene; AT5G05450.1; AT5G05450.1; AT5G05450.
DR KEGG; ath:AT5G05450; -.
DR Araport; AT5G05450; -.
DR TAIR; locus:2153564; AT5G05450.
DR eggNOG; KOG0345; Eukaryota.
DR HOGENOM; CLU_003041_26_4_1; -.
DR InParanoid; Q9FLB0; -.
DR OMA; DRAIHDK; -.
DR OrthoDB; 973872at2759; -.
DR PhylomeDB; Q9FLB0; -.
DR PRO; PR:Q9FLB0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FLB0; baseline and differential.
DR Genevisible; Q9FLB0; AT.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR025313; DUF4217.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF13959; DUF4217; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM01178; DUF4217; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW RNA-binding.
FT CHAIN 1..593
FT /note="DEAD-box ATP-dependent RNA helicase 18"
FT /id="PRO_0000239160"
FT DOMAIN 47..226
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 264..411
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 506..561
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 16..44
FT /note="Q motif"
FT MOTIF 174..177
FT /note="DEAD box"
FT COMPBIAS 506..545
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 60..67
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT CONFLICT 258
FT /note="A -> R (in Ref. 3; CAA09208)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 593 AA; 66939 MW; 3CF37AACEA98BAA2 CRC64;
MDSSANINKA LTETRFSDLE PPLSGDIIEA LNQSDFEFCT PVQAATIPLL CSYKDVAVDA
ATGSGKTLAF VVPLVEILRR STSFPPKPHQ VMGVIISPTR ELSTQIYNVA QPFVSTLANV
NSVLLVGGRE VKADMKIIEE EGCNVLIGTP GRLSDIMERM EILDFRNLEI LILDEADRLL
EMGFQRQVNY IISRLPKQRR TGLFSATQTE GVEELAKAGL RNPVRVEVRA KSKSESSQQL
TNSKTPSGLH LEYMECEADK KSSQLVDLLI KNSDKKLIVF FMTCASVDYW GLVLSKIPAL
KSISLIPIHG DMKQNARDKA LASFTKASSG ALLCTDVAAR GLDIPGIDYV VQYDPPQDPN
MFNHRAGRTA RLGRQGRAIV FLLPKEEAYV EFMRIRRVPL EERKCSEDAS DVIPIIRSAA
MKDRAVMEKG LKAFVSFVRA YKEHHCSFIF RWKDLEIGKL AMGYGLLYLP SMSEVKQHRL
SSEGFTPVEG VKFEEIKFKD KYREKQRQQN LQVRKEKRQE EKKEKGKRKR VDASASNDPK
KASRKLTGKQ RQTIQTAEDE EVMDRDYKLM IKVKKGLIKE DEYERLTGDD DLF
