RH22_ARATH
ID RH22_ARATH Reviewed; 581 AA.
AC Q944S1; O24597; Q9ZNU1;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 22;
DE EC=3.6.4.13;
GN Name=RH22; OrderedLocusNames=At1g59990; ORFNames=T2K10.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 77-581.
RC STRAIN=cv. Columbia;
RX PubMed=9862990; DOI=10.1093/nar/27.2.628;
RA Aubourg S., Kreis M., Lecharny A.;
RT "The DEAD box RNA helicase family in Arabidopsis thaliana.";
RL Nucleic Acids Res. 27:628-636(1999).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 74-275.
RC STRAIN=cv. Columbia; TISSUE=Shoot;
RX PubMed=9358062; DOI=10.1016/s0378-1119(97)00374-0;
RA Aubourg S., Takvorian A., Cheron A., Kreis M., Lecharny A.;
RT "Structure, organization and putative function of the genes identified
RT within a 23.9 kb fragment from Arabidopsis thaliana chromosome IV sequenced
RT in the framework of the ESSA programme.";
RL Gene 199:241-253(1997).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17168887; DOI=10.1111/j.1467-7652.2004.00084.x;
RA Mingam A., Toffano-Nioche C., Brunaud V., Boudet N., Kreis M., Lecharny A.;
RT "DEAD-box RNA helicases in Arabidopsis thaliana: establishing a link
RT between quantitative expression, gene structure and evolution of a family
RT of genes.";
RL Plant Biotechnol. J. 2:401-415(2004).
RN [7]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD14475.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC005966; AAD14475.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE33647.1; -; Genomic_DNA.
DR EMBL; AF424570; AAL11564.1; -; mRNA.
DR EMBL; AY142022; AAM98286.1; -; mRNA.
DR EMBL; AJ010471; CAA09210.1; -; mRNA.
DR EMBL; Y11190; CAA72075.1; -; mRNA.
DR PIR; B96624; B96624.
DR PIR; T51346; T51346.
DR RefSeq; NP_176207.1; NM_104691.4.
DR AlphaFoldDB; Q944S1; -.
DR SMR; Q944S1; -.
DR BioGRID; 27518; 4.
DR STRING; 3702.AT1G59990.1; -.
DR PaxDb; Q944S1; -.
DR PRIDE; Q944S1; -.
DR ProteomicsDB; 236942; -.
DR EnsemblPlants; AT1G59990.1; AT1G59990.1; AT1G59990.
DR GeneID; 842293; -.
DR Gramene; AT1G59990.1; AT1G59990.1; AT1G59990.
DR KEGG; ath:AT1G59990; -.
DR Araport; AT1G59990; -.
DR TAIR; locus:2202730; AT1G59990.
DR eggNOG; KOG0333; Eukaryota.
DR HOGENOM; CLU_003041_1_8_1; -.
DR InParanoid; Q944S1; -.
DR OMA; DFQQKGG; -.
DR OrthoDB; 1223767at2759; -.
DR PhylomeDB; Q944S1; -.
DR PRO; PR:Q944S1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q944S1; baseline and differential.
DR Genevisible; Q944S1; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW RNA-binding.
FT CHAIN 1..581
FT /note="DEAD-box ATP-dependent RNA helicase 22"
FT /id="PRO_0000239163"
FT DOMAIN 111..380
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 408..566
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 80..108
FT /note="Q motif"
FT MOTIF 244..247
FT /note="DEAD box"
FT BINDING 124..131
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT CONFLICT 76
FT /note="A -> F (in Ref. 5; CAA72075)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 581 AA; 64747 MW; 67F9F595E515257D CRC64;
MILSRSVSVL HLCGVSSSAP SKLLSQRFKV SFALAYGSSV SFRLSSLNRS DRKWVRGFAS
ATEAEVEKKG NDTFFADHTV SWKSLGLSDN VSIALRDSGF DRPSLTQAVC IPSILSGKDV
IVAAETGSGK THGYLAPIID QLTNTALDSE VTNREERPFP LKNISLILCP NVMLCEQVVR
MVNGLVDEDG NPLLRVEAVC GSQGWPDRLP DIIVSTPAAL LNNIEPKRNR RLEFLRCVKY
VVFDEADMLL CGSFQNQIIR LINMLRFDEK QVSRLAKSNL GRPMEIDASV PQIDLENEDD
AEFDEGSISE EEDEEEEEEY LDDIAQMPSV EAEAGSDTKK GWRRVRKIYT RSKQYIFIAA
TLPVNGKKTA GGILKHMFQD AVWVSGNFLH RNSPRLKQKW VEVTVDSQVD ALIEAVKNNN
NTNTERTMVF ANTVEAVEAV ADILEKASIQ CYRYHKNHKL DERANILADF RETGGVFVCT
DAAARGVDVP NVSHVIQADF ASSAVDFLHR IGRTARAGQY GTVTSLYTEA NRDLVEAIRE
AVKMGQPVET AFSRKRGFRN KVKKRAFLKA EEAEEPQAVR Y
