RH22_ORYSJ
ID RH22_ORYSJ Reviewed; 577 AA.
AC Q6H601;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 22;
DE EC=3.6.4.13;
GN OrderedLocusNames=Os09g0383400, LOC_Os09g21520; ORFNames=P0505H05.40;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-577 (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6H601-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6H601-2; Sequence=VSP_024170, VSP_024171;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AK103385; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAD25848.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF24981.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP005312; BAD25848.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008215; BAF24981.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014965; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK103385; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015651427.1; XM_015795941.1. [Q6H601-1]
DR AlphaFoldDB; Q6H601; -.
DR SMR; Q6H601; -.
DR STRING; 4530.OS09T0383400-01; -.
DR PaxDb; Q6H601; -.
DR PRIDE; Q6H601; -.
DR GeneID; 4346941; -.
DR KEGG; osa:4346941; -.
DR eggNOG; KOG0333; Eukaryota.
DR HOGENOM; CLU_003041_1_8_1; -.
DR InParanoid; Q6H601; -.
DR OrthoDB; 1223767at2759; -.
DR Proteomes; UP000000763; Chromosome 9.
DR Proteomes; UP000059680; Chromosome 9.
DR Genevisible; Q6H601; OS.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Helicase; Hydrolase; Nucleotide-binding;
KW Reference proteome; RNA-binding.
FT CHAIN 1..577
FT /note="DEAD-box ATP-dependent RNA helicase 22"
FT /id="PRO_0000282511"
FT DOMAIN 113..375
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 407..568
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 288..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 82..110
FT /note="Q motif"
FT MOTIF 249..252
FT /note="DEAD box"
FT COMPBIAS 288..303
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 126..133
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT VAR_SEQ 500
FT /note="A -> V (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_024170"
FT VAR_SEQ 501..577
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_024171"
SQ SEQUENCE 577 AA; 63255 MW; AA5A3FD5F87B9CD1 CRC64;
MALHHLRHAP LALRLARLPR LAPSPPPPAA RLLLLLAPSQ HPAPPWRLLS RPRALATAAA
EADDAGAGGN GDGDGFFSEE STSWESLGVS DRLASALHGA GLARPSLVQA ACIPHVLTTN
DVIVAAETGS GKTHGYLVPL IEKLCSKSIS AEDGNSQDVT SGSPNIALVL CPNVMLCEQV
VRMANSLVDE SGEPLKSAAA VCGPKGWPTV RPDILVATPA ALLNYLFDYD PEKRRRERFL
RNVKFIVFDE ADMLLCGSFE NQVIRLIHML RFDEKLLSRM EDSGKEISLG DTNEYREDSD
SQSAELSADD EENEDGLVQH RPVNVENAHI GAHKKDWRRV RKVYRRSKQY VFVAATLPQS
GKKTAGGVLK RMFPNAVWVS GAYLHRHNPR LERRWIEVTA DTQVSALLDA VKYGLKNEVH
DTKLGPNRTM VFTNTVDAAN SVSDILQRVG VPCILYHRDS SLEERAKNLQ SFRENGGVLV
CTDAAARGLD VPNVSHVIQA EFAACAVDFL HRVGRTARAG QSGIVTSLYT EANRDLVRAV
RQAEELAQPV EKAFSRKRSF RNKLKKQALH KSTALLS
