RH24_ORYSJ
ID RH24_ORYSJ Reviewed; 770 AA.
AC Q10MH8; B7EVA9;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 24;
DE EC=3.6.4.13;
GN OrderedLocusNames=Os03g0308500, LOC_Os03g19530;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. {ECO:0000305}.
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DR EMBL; DP000009; ABF95549.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF11818.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS83834.1; -; Genomic_DNA.
DR EMBL; AK103891; BAG96306.1; -; mRNA.
DR RefSeq; XP_015633092.1; XM_015777606.1.
DR AlphaFoldDB; Q10MH8; -.
DR SMR; Q10MH8; -.
DR STRING; 4530.OS03T0308500-01; -.
DR PaxDb; Q10MH8; -.
DR PRIDE; Q10MH8; -.
DR EnsemblPlants; Os03t0308500-01; Os03t0308500-01; Os03g0308500.
DR GeneID; 4332617; -.
DR Gramene; Os03t0308500-01; Os03t0308500-01; Os03g0308500.
DR KEGG; osa:4332617; -.
DR eggNOG; KOG0339; Eukaryota.
DR HOGENOM; CLU_003041_9_1_1; -.
DR InParanoid; Q10MH8; -.
DR OMA; FRSQYNS; -.
DR OrthoDB; 245118at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q10MH8; OS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW RNA-binding.
FT CHAIN 1..770
FT /note="DEAD-box ATP-dependent RNA helicase 24"
FT /id="PRO_0000282500"
FT DOMAIN 250..425
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 436..599
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 604..640
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 683..704
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 729..770
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 219..247
FT /note="Q motif"
FT MOTIF 373..376
FT /note="DEAD box"
FT COMPBIAS 683..700
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 753..770
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 263..270
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 770 AA; 83185 MW; AD9D5FF6AA5C54D6 CRC64;
MSKRPKLGGF SIPRPTSYSF ERSQPPQRLY VPADDPDLDD IAFSDDAAAP SDAPPAGGGG
AAGDEEEIDP LDAFMAEIQE EIRAPPPAPK PEALRRADSD DEDDPVESFL RAKKDSGLAL
AADAMHAGYD SDEEVYAAAK AVDAGMMEYD SDDNPIVVDK KKIEPIPPLD HSTIEYEPFN
KDFYEEKPSV SGMSEQEVAD YMKSLAIRVS GFDVPRPIKS FADCGFPVQL MNAIAKQGYE
KPTTIQCQAL PIVLSGRDII GIAKTGSGKT AAFVLPMIVH IMDQPELEKE EGPIGVVCAP
TRELAHQIYL EAKKFAKPYN LRVAAVYGGV SKFDQFKELK AGCEIVIATP GRLIDLLKMK
ALKMFRATYL VLDEADRMFD LGFEPQIRSI VGQIRPDRQT LLFSATMPYK VERLAREILT
DPIRVTVGQV GSANEDIKQV VNVLPSDAEK MPWLLEKLPG MIDDGDVLVF AAKKARVDEI
ESQLNQRGFR IAALHGDKDQ ASRMETLQKF KSGVYHVLVA TDVAARGLDI KSIKTVVNFD
IAKEMDMHIH RIGRTGRAGD KDGTAYTLIT QKEVRFAGEL VHCLIAAGQD VPNELMDLAM
KDGRFRANRD SRKGGKKSGK GKGGGGGGGG GSGARGRGRG VRGVDFGLGI GYNAESGSVP
APRSAALNSL KTGMMQNFKS SFVSASSSNT PSNSAPSRGA PSSFVRPALR GFVSGGTIGG
DANQARAVLP APSFVPASRP AENTVENANP NPESSRDRTR ERKRPSGWDR
