ID   RH28_ORYSJ              Reviewed;         802 AA.
AC   Q0INC5; B7ELR4; Q2QQW2;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 2.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase 28;
DE            EC=3.6.4.13;
GN   OrderedLocusNames=Os12g0481100, LOC_Os12g29660; ORFNames=OsJ_36091;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG   The rice chromosomes 11 and 12 sequencing consortia;
RT   "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT   genes and recent gene duplications.";
RL   BMC Biol. 3:20-20(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC       family of RNA helicases and controls ATP binding and hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX27/DRS1
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF29790.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; DP000011; ABA98487.1; -; Genomic_DNA.
DR   EMBL; AP008218; BAF29790.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP014968; BAT17143.1; -; Genomic_DNA.
DR   EMBL; CM000149; EAZ20482.1; -; Genomic_DNA.
DR   EMBL; AK073151; BAG93311.1; -; mRNA.
DR   RefSeq; XP_015620340.1; XM_015764854.1.
DR   AlphaFoldDB; Q0INC5; -.
DR   SMR; Q0INC5; -.
DR   STRING; 4530.OS12T0481100-01; -.
DR   PaxDb; Q0INC5; -.
DR   PRIDE; Q0INC5; -.
DR   EnsemblPlants; Os12t0481100-01; Os12t0481100-01; Os12g0481100.
DR   GeneID; 4352218; -.
DR   Gramene; Os12t0481100-01; Os12t0481100-01; Os12g0481100.
DR   KEGG; osa:4352218; -.
DR   eggNOG; KOG0338; Eukaryota.
DR   HOGENOM; CLU_003041_3_3_1; -.
DR   InParanoid; Q0INC5; -.
DR   OMA; AAHTDIR; -.
DR   OrthoDB; 268859at2759; -.
DR   Proteomes; UP000000763; Chromosome 12.
DR   Proteomes; UP000007752; Chromosome 12.
DR   Proteomes; UP000059680; Chromosome 12.
DR   Genevisible; Q0INC5; OS.
DR   GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Coiled coil; Helicase; Hydrolase; Nucleotide-binding;
KW   Reference proteome; RNA-binding.
FT   CHAIN           1..802
FT                   /note="DEAD-box ATP-dependent RNA helicase 28"
FT                   /id="PRO_0000282488"
FT   DOMAIN          225..399
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          429..573
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   REGION          1..179
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          639..802
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          90..122
FT                   /evidence="ECO:0000255"
FT   COILED          149..174
FT                   /evidence="ECO:0000255"
FT   COILED          572..616
FT                   /evidence="ECO:0000255"
FT   COILED          671..712
FT                   /evidence="ECO:0000255"
FT   MOTIF           194..222
FT                   /note="Q motif"
FT   MOTIF           347..350
FT                   /note="DEAD box"
FT   COMPBIAS        77..168
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        643..658
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        702..719
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        738..757
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        779..802
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         238..245
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   802 AA;  89508 MW;  0EFE65D3C817B71E CRC64;
     MDADFRFDPD GSDDDGAAAS AAAGRRKPAQ SPWEFSSYAE SVAAEHARRR TTSIDEKISQ
     ALSGSRRGGK PSIPDGDSEG DEDDSEVEDD SEEDDKEVVE GEIDDEEDEV EESEDDDEGV
     EVSDEEVEEL EEGKGEEKSD EVEEGEEGQD GEEEEKEEGD EEAAEEEEET DKKSGVVDPS
     KFFASSEGAS FHANSFLELN LSRPLLRACE ALGYQKPTPI QAACIPLALT GRDICGSAIT
     GSGKTAAFSL PVLERLLFRP KRVPAIRVLI LTPTRELAAQ VHSMIEKLAQ FTDIRCCLIV
     GGLSTKVQEV ALRSMPDIVV ATPGRIIDHL RNSLSVGLED LAILILDEAD RLLELGFSAE
     IQELIRMCPR RRQTMLFSAT MTEEINELVT LSLNKPVRLE ADPSLKRPAT LTEEVVRIRR
     AREANQEAVL LALCLKTFKD KVIIFSGTKH SAHRLKIIFG LSGMKAAELH GNLTQAQRLE
     ALELFKKQEV DFLIATDVAA RGIDIVGVRT VINFSCPRDA RTYLHRVGRT ARAGREGYAV
     TFVTDDDRSL LKAIAKKAGS QLKSRIVAEK PVAECAKLIE ELEDQISTII QEEREERILR
     KAEMEATKAE NMIAHKDEIY SRPKRTWFAT EKEKKLLAKA AKESTSQGKS NSGVISAQQA
     EDLRLKEKKR REREKNLPRK KRRRLEAERE MLEDESEDEE EAKESKGGKK EKKGQSLVDV
     AYRRAKSMKA SGKRGAGTGK GKNDKKAKQH SGKGPTRQEE MQELFQNDMS EWKQGRSLKK
     NNVMRKKSKN SFKSKSRYNR RK