RH29_ORYSJ
ID RH29_ORYSJ Reviewed; 851 AA.
AC A3BT52; B9G0W9; Q0J5P3; Q68Q05; Q6ZA73;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 29;
DE Short=RNAH;
DE EC=3.6.4.13;
GN OrderedLocusNames=Os08g0416100, LOC_Os08g32090;
GN ORFNames=OsJ_026224, OsJ_27320 {ECO:0000312|EMBL:EEE68686.1}, P0433E10.15;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX54/DBP10
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC98579.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AP004667; BAC98579.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP008214; BAF23722.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT05437.1; -; Genomic_DNA.
DR EMBL; CM000145; EEE68686.1; -; Genomic_DNA.
DR EMBL; AK070406; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015650417.1; XM_015794931.1.
DR AlphaFoldDB; A3BT52; -.
DR SMR; A3BT52; -.
DR STRING; 4530.OS08T0416100-01; -.
DR PaxDb; A3BT52; -.
DR PRIDE; A3BT52; -.
DR EnsemblPlants; Os08t0416100-01; Os08t0416100-01; Os08g0416100.
DR GeneID; 4345577; -.
DR Gramene; Os08t0416100-01; Os08t0416100-01; Os08g0416100.
DR KEGG; osa:4345577; -.
DR eggNOG; KOG0337; Eukaryota.
DR HOGENOM; CLU_003041_5_2_1; -.
DR InParanoid; A3BT52; -.
DR OMA; MRWDKKS; -.
DR OrthoDB; 268859at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR Genevisible; A3BT52; OS.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003729; F:mRNA binding; IEA:EnsemblPlants.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR012541; DBP10_C.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF08147; DBP10CT; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM01123; DBP10CT; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW RNA-binding.
FT CHAIN 1..851
FT /note="DEAD-box ATP-dependent RNA helicase 29"
FT /id="PRO_0000282507"
FT DOMAIN 80..253
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 277..426
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 719..851
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 49..77
FT /note="Q motif"
FT MOTIF 201..204
FT /note="DEAD box"
FT COMPBIAS 28..46
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 771..827
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 93..100
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT CONFLICT 295
FT /note="T -> S (in Ref. 5; AK070406)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 851 AA; 95532 MW; D9E5E29A01BA2A84 CRC64;
MARLNPSKPS SRGGKPRSSS ADAMAEHKPL PGRPKREGEG ASKRKAKSGG FESMGLCEEV
YRGVRHKGYR VPTPIQRKAM PLILAGHDIA AMARTGSGKT AAFLVPMIQR LRRHDAGAGI
RALILSPTRD LATQTLKFAQ QLGKFTDLKI SLIVGGDSME SQFEELAENP DIIIATPGRL
VHHLAEVEDL NLRTVEYVVF DEADSLFSLG LIQQLHDILH KLSDTRQTLL FSATLPQALA
DFAKAGLRDP QIVRLDLDKK ISPDLKLAFF TLRQEEKLAA LLYLVRERIS SEEQTIIFVS
TKHHVEFLNI LFREEGLEPS LSYGAMDQEA RNIHISKFRA RKTMILIVTD VAARGLDIPL
LDNVVNWDFP AKPKLFVHRV GRVARQGRSG TAYTFVTSED MAYLLDLHLF LSKPLRPAPT
EEELLKDMEG MNLKIDRALA NGETVYGRFP QTIIDLVSDG IKEVINGCTD LIALEKPCTN
AFHLYLKTRP MPSTESIRRV KDLPREGLHP IFRDVLGSDE LSALAFSERL KSFRPKQTIL
EAEGEAARGS NQWLDVMKKK REVHEGIINL VHQKNNVDHE PKEELVENIS NWERKDVCGN
KRKLQSFRDE EYYISSVPQN QHLEAGLSVR ANEGFVENRL DAAVLDLVDD ETSGMQAQKT
RYHWKKNKFV KLNSGDRVTA TGKIKTESGA KLKPTKTGIY KKWQQKTHRS IDTGRKFGGF
AEEGASTTGS HQRGNRKHTA VGRGRRYIPN ADVPSEIRNP EQIQKSRQQK AMDIARMKNR
STKESKFQKF QKNNRRHDGP LKDGKFQKNR RPDGNGKNRR PDGNGKGRGK GKGNANGFGK
GKGKMKGKGT R
