RH31_ARATH
ID RH31_ARATH Reviewed; 716 AA.
AC Q9FFQ1; F4KAS7; Q9ZRZ6;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 31;
DE EC=3.6.4.13;
GN Name=RH31; OrderedLocusNames=At5g63630; ORFNames=MBK5.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 326-620.
RC STRAIN=cv. Columbia;
RX PubMed=9862990; DOI=10.1093/nar/27.2.628;
RA Aubourg S., Kreis M., Lecharny A.;
RT "The DEAD box RNA helicase family in Arabidopsis thaliana.";
RL Nucleic Acids Res. 27:628-636(1999).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17168887; DOI=10.1111/j.1467-7652.2004.00084.x;
RA Mingam A., Toffano-Nioche C., Brunaud V., Boudet N., Kreis M., Lecharny A.;
RT "DEAD-box RNA helicases in Arabidopsis thaliana: establishing a link
RT between quantitative expression, gene structure and evolution of a family
RT of genes.";
RL Plant Biotechnol. J. 2:401-415(2004).
RN [5]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10456.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB005234; BAB10456.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002688; ANM68626.1; -; Genomic_DNA.
DR EMBL; AJ010477; CAA09216.1; -; mRNA.
DR PIR; T51350; T51350.
DR RefSeq; NP_001318870.1; NM_001345599.1.
DR RefSeq; NP_201168.3; NM_125758.3.
DR AlphaFoldDB; Q9FFQ1; -.
DR SMR; Q9FFQ1; -.
DR STRING; 3702.AT5G63630.1; -.
DR iPTMnet; Q9FFQ1; -.
DR PaxDb; Q9FFQ1; -.
DR PRIDE; Q9FFQ1; -.
DR ProteomicsDB; 237000; -.
DR EnsemblPlants; AT5G63630.2; AT5G63630.2; AT5G63630.
DR GeneID; 836483; -.
DR Gramene; AT5G63630.2; AT5G63630.2; AT5G63630.
DR KEGG; ath:AT5G63630; -.
DR Araport; AT5G63630; -.
DR TAIR; locus:2160639; AT5G63630.
DR eggNOG; KOG0342; Eukaryota.
DR HOGENOM; CLU_003041_26_6_1; -.
DR InParanoid; Q9FFQ1; -.
DR OrthoDB; 537587at2759; -.
DR PhylomeDB; Q9FFQ1; -.
DR PRO; PR:Q9FFQ1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FFQ1; baseline and differential.
DR Genevisible; Q9FFQ1; AT.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW RNA-binding.
FT CHAIN 1..716
FT /note="DEAD-box ATP-dependent RNA helicase 31"
FT /id="PRO_0000239171"
FT DOMAIN 279..462
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 497..643
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 99..188
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 248..276
FT /note="Q motif"
FT MOTIF 410..413
FT /note="DEAD box"
FT COMPBIAS 99..145
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 152..188
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 292..299
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 716 AA; 81083 MW; 8CCEAB5FBAFC7807 CRC64;
MPLNFPLRIR FFAHSLSGTH LSYNTSSSVP LLFRIFSSGL NHFEFGSRIN FSTRPNRDQP
EFERRIRDGG EIRASKSLIE DEEELSNWVS GFRTGSSRGI LKSDDEDEED RSRGRNQEKR
GIRNQVDSFR NKRYGGDRER GFNSRIQGKS SEASFRGRKE TSFSRDREDE KGLRKREDLR
LEDESSDEDV KSLVMGDIGD LLSEDDEEED QDYDFLKKKA VSAFGFDKEN VIEADKTRNA
NDSYLTKTRF DHYPLSPLSL KAIKDAGYET MTVVQEATLP IILKGKDVLA KAKTGTGKTV
AFLLPSIEVV VKSPPTSPDN KRPPILALVI CPTRELANQA ATEANTLLKY HPSIGVQVVI
GGTRLGLEQK RMQTNPCQIL VATPGRLKDH IENTPGFATR LKGVKVLVLD EADHLLDMGF
RKDIERIISA VPKERQTFLF SATVPEEVRQ ICLVALRRDH EFVNCVHEGT IETHQQVRQM
HMIASLDRHF SLLYTLLREH IMGNVDYKVI VFCTTAMVTK LVADLLGELN LNVREIHSRK
PQSYRTRVSN EFRKSKGLIL VTSDVSARGV DYPDVTLVLQ VGLPKDREQY IHRLGRTGRK
GKEGEGILLL APWEEYFLSS LKDLPITKSP LPSIDPETVK KVQKALCHVE MRNKEAAYQA
WLGYYNSQKM IGRDKDRLVE LANEFSRSMG LDNPPAIPKL ILGKMGLKNV PGLRAK
