ID   RH32_ORYSJ              Reviewed;         773 AA.
AC   Q0D622; Q84Z30;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 2.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase 32;
DE            EC=3.6.4.13;
GN   OrderedLocusNames=Os07g0517000, LOC_Os07g33340;
GN   ORFNames=OSJNBa0036M16.110, P0650C03.25;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 250-773.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC       family of RNA helicases and controls ATP binding and hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX10/DBP4
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF21701.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP005103; BAC83715.1; -; Genomic_DNA.
DR   EMBL; AP005320; BAD31318.1; -; Genomic_DNA.
DR   EMBL; AP008213; BAF21701.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014963; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK064167; -; NOT_ANNOTATED_CDS; mRNA.
DR   AlphaFoldDB; Q0D622; -.
DR   SMR; Q0D622; -.
DR   STRING; 4530.OS07T0517000-01; -.
DR   PaxDb; Q0D622; -.
DR   PRIDE; Q0D622; -.
DR   eggNOG; KOG0343; Eukaryota.
DR   HOGENOM; CLU_003041_26_1_1; -.
DR   InParanoid; Q0D622; -.
DR   Proteomes; UP000000763; Chromosome 7.
DR   Proteomes; UP000059680; Chromosome 7.
DR   Genevisible; Q0D622; OS.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR025313; DUF4217.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF13959; DUF4217; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM01178; DUF4217; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Coiled coil; Helicase; Hydrolase; Nucleotide-binding;
KW   Reference proteome; RNA-binding.
FT   CHAIN           1..773
FT                   /note="DEAD-box ATP-dependent RNA helicase 32"
FT                   /id="PRO_0000282465"
FT   DOMAIN          111..287
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          309..462
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   REGION          28..71
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          699..755
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          664..715
FT                   /evidence="ECO:0000255"
FT   MOTIF           80..108
FT                   /note="Q motif"
FT   MOTIF           235..238
FT                   /note="DEAD box"
FT   COMPBIAS        706..755
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         124..131
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   773 AA;  86827 MW;  9E8E1B50A5EF0F34 CRC64;
     MRRPRSRGAA KQTRLREADE IRLLEAWIDA GKPARGTRPP PLSKSSSSPA DTAAAKRGAK
     GAGGVPSKAA GEHPEYGACA RFDELPLSNK TKDGLRKAGY TEMSEIQRAA LPHALCGRDV
     LGAAKTGSGK TLAFVIPVLE KLYRERWGPE DGVGCIVLSP NKDLAGQIFN VFQKVGKLHG
     FSAACIVGNR KGLDEEKAVI NNMNILVCTP GRLLQHMGET TNFDCSQIQQ ILVIDEADQV
     LDKNFQEQVD NVVSQLPKVR QTLLFSATQT KSVKDLARVS LKDPEYISVH EEATTATPDT
     LEQYAMIVPL EQKLNMLWSF IKRHLKSRIL VFLSSVKQVK FVYEVFKKLR PGISLRCMHG
     RMKYEVQQAI VAEFKEGHSV LFSTDIFARG LDIEDVDWVV QVDCPENIAL YIHRVGRTAR
     YNKRGKALIF LCPEEEKMLE KLKAAESKIP IHIKKPNTEQ LQQISQNIAS VLVQYPNLQQ
     LGKRAFVTYL KSVYLQSDKE VFDLSRFSME NFAAYAASLG LPVTPKIRFV SHKKNVPKKY
     MGDIDVKRMK RSSKPEVIEI NPQAKSNLIE DDGDYDILYP KEQQTDVNMA DGLDDVLYPK
     VSTADTNNEP EKVTQLGNKS VKKKKLKINV HRPLGTRVKF DDEGHTIPPF ASIAEEVGSG
     DVIDKDKISQ RYAEMLREMQ EHDKEDKLEH KRILREKKLQ KKLKLKRKRN EEMDAGSENS
     GSESDRDQRT ASKGKKRYFN SDDEEGSKDA AKDGDVLAQQ EALALKLLSK MHS