RH33_ARATH
ID RH33_ARATH Reviewed; 845 AA.
AC O80792;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Putative DEAD-box ATP-dependent RNA helicase 33;
DE EC=3.6.4.13;
GN Name=RH33; OrderedLocusNames=At2g07750; ORFNames=T12J2.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17168887; DOI=10.1111/j.1467-7652.2004.00084.x;
RA Mingam A., Toffano-Nioche C., Brunaud V., Boudet N., Kreis M., Lecharny A.;
RT "DEAD-box RNA helicases in Arabidopsis thaliana: establishing a link
RT between quantitative expression, gene structure and evolution of a family
RT of genes.";
RL Plant Biotechnol. J. 2:401-415(2004).
RN [4]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC004483; AAC26676.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06130.1; -; Genomic_DNA.
DR PIR; E84488; E84488.
DR RefSeq; NP_178818.1; NM_126777.1.
DR AlphaFoldDB; O80792; -.
DR SMR; O80792; -.
DR BioGRID; 811; 1.
DR STRING; 3702.AT2G07750.1; -.
DR iPTMnet; O80792; -.
DR PaxDb; O80792; -.
DR PRIDE; O80792; -.
DR EnsemblPlants; AT2G07750.1; AT2G07750.1; AT2G07750.
DR GeneID; 815422; -.
DR Gramene; AT2G07750.1; AT2G07750.1; AT2G07750.
DR KEGG; ath:AT2G07750; -.
DR Araport; AT2G07750; -.
DR TAIR; locus:2054688; AT2G07750.
DR eggNOG; KOG0342; Eukaryota.
DR HOGENOM; CLU_003041_26_6_1; -.
DR InParanoid; O80792; -.
DR OMA; YNSNISW; -.
DR OrthoDB; 537587at2759; -.
DR PhylomeDB; O80792; -.
DR PRO; PR:O80792; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O80792; baseline.
DR Genevisible; O80792; AT.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW RNA-binding.
FT CHAIN 1..845
FT /note="Putative DEAD-box ATP-dependent RNA helicase 33"
FT /id="PRO_0000239173"
FT DOMAIN 406..590
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 624..778
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 129..149
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 282..302
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 375..403
FT /note="Q motif"
FT MOTIF 538..541
FT /note="DEAD box"
FT BINDING 419..426
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 845 AA; 94886 MW; 3EABCE5EC10D3F03 CRC64;
MAMAMRLPAI SRAVTEVASS PVGLRRLFCS NASRFSFLSP PARRQAEPST NLFHSGLSKR
ITSERSLWNR IFSRNMGGGP RTFPGGLNKW QWKRMHEKKA REKENKLLDQ EKQLYEARIR
TEIRAKMWGH PDSGEKTAKL KQSHGPMSPK EHIKTLADRF MKAGADDLWN DNDGPVKKFD
QGSRSCSDSI DSTPIDVRRL VSATCDSMGK HRVLDSSRRG FSSMSRFKRN ESSCDEGDDV
DAKKLDTLSP FSPKFSGTKE KVKSSTSVVG VIRNKGLFGR RKFRKNDSST EEDSDEEGNE
GKMIGWMDLR KTGSSASLGN HDIKLTKRVN RNVTDEELYP PLDINRVRED LSKKQSVDNV
MEEKQEPHDS IYSAKRFDES CISPLTLKAL SASGIVKMTR VQDATLSECL DGKDALVKAK
TGTGKSMAFL LPAIETVLKA MNSGKGVHKV APIFVLILCP TRELASQIAA EGKALLKNHD
GIGVQTLIGG TRFRLDQQRL ESEPCQILIA TPGRLLDHIE NKSGLTSRLM ALKLFIVDEA
DLLLDLGFKR DVEKIIDCLP RQRQSLLFSA TIPKEVRRVS QLVLKRDHSY IDTIGLGCVE
THDKVKQSCI VAPHESHFHL VPHLLKEHIN NMPDYKIIVF CSTGMVTSLM YTLLREMKLN
VREIHARKPQ LHRTCVSDEF KESNRLILVT SDVSARGMNY PDVTLVIQVG IPSDREQYIH
RLGRTGREGK GGKGLLLIAP WERYFLDELK DLPLEPIPAP DLDSRVKHQV DQSMAKIDTS
IKEAAYHAWL GYYNSVRETG RDKTTLAELA NRFCHSIGLE KPPALFRRTA VKMGLKGISG
IPIRK
