RH35A_ORYSJ
ID RH35A_ORYSJ Reviewed; 627 AA.
AC Q0E3X4; Q67UX4;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 35A;
DE EC=3.6.4.13;
GN OrderedLocusNames=Os02g0150100, LOC_Os02g05660; ORFNames=OSJNBa0050G13.7;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 168-627.
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX41 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF07814.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AP005412; BAD38045.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF07814.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014958; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK060595; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015626564.1; XM_015771078.1.
DR AlphaFoldDB; Q0E3X4; -.
DR SMR; Q0E3X4; -.
DR STRING; 4530.OS02T0150100-02; -.
DR PaxDb; Q0E3X4; -.
DR PRIDE; Q0E3X4; -.
DR KEGG; osa:4328308; -.
DR eggNOG; KOG0341; Eukaryota.
DR HOGENOM; CLU_003041_16_5_1; -.
DR InParanoid; Q0E3X4; -.
DR OrthoDB; 447398at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q0E3X4; OS.
DR GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd17951; DEADc_DDX41; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR044113; DEADc_DDX41.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR InterPro; IPR001878; Znf_CCHC.
DR InterPro; IPR036875; Znf_CCHC_sf.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00343; ZnF_C2HC; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF57756; SSF57756; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
DR PROSITE; PS50158; ZF_CCHC; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Metal-binding; Nucleotide-binding;
KW Reference proteome; RNA-binding; Zinc; Zinc-finger.
FT CHAIN 1..627
FT /note="DEAD-box ATP-dependent RNA helicase 35A"
FT /id="PRO_0000282498"
FT DOMAIN 213..397
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 408..568
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT ZN_FING 584..601
FT /note="CCHC-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 40..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 182..210
FT /note="Q motif"
FT MOTIF 345..348
FT /note="DEAD box"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 60..74
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 226..233
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 627 AA; 69242 MW; 7D42E609E57B2909 CRC64;
MAAATASSPA TAAAANSDDE DNYEEYIPVA KRRAMEADRL RRLRLSKPAP PSSSAAEAAS
DLPPPPPPPP NQPSAGGGGG GLEASAKPSL LVKATQLKRA APEVTHTEQL IMQEKEMIEH
LSDRKTLMSV RELAKGITYS DPLKTGWKPP LRLRRMPRAK ADELRRKWHI LVDGDDVPPP
ARDFRDLRLP EPMLRKLREK GIVQPTPIQV QGLPVVLSGR DMIGIAFTGS GKTLVFVLPL
IMVALQEEMM MPIVPGEGPF GMIICPSREL AKQTYDVIEQ FLVPLKEAGY PEIRPLLCIG
GVDMRAQLDV VKKGVHIVVA TPGRLKDLLA KKKMNLDNCR YLTLDEADRL VDLGFEDDIR
EVFDHFKAQR QTLLFSATMP KKIQNFAKSA LVKPVIVNVG RAGAANLDVI QEVEYVKEDA
RIIYLLECLQ KTPPPVLVFC ENKADVDYIH EYLLLKGVEA VAIHGGKDQE ERENAIEFFK
NGKKDVLVAT DVASKGLDFP DIQHVINYDM PAEIENYVHR IGRTGRCGKT GIATTFINKN
QTETTLLDLK HLLKEAKQRI PPVLAELNDP LEDEETMAKE SGVKGCAYCG GLGHRVTDCP
KLEHQKSMAI AGSRRDYYGG GGYRGEI
