ID   RH35B_ORYSJ             Reviewed;         619 AA.
AC   Q5Z6G5; A0A0P0X0D6;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase 35B;
DE            EC=3.6.4.13;
GN   OrderedLocusNames=Os06g0697200, LOC_Os06g48210;
GN   ORFNames=OsJ_22496, P0028E05.25;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC       family of RNA helicases and controls ATP binding and hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX41 subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF20374.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP005445; BAD54454.1; -; Genomic_DNA.
DR   EMBL; AP008212; BAF20374.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014962; BAS99296.1; -; Genomic_DNA.
DR   EMBL; CM000143; EAZ38145.1; -; Genomic_DNA.
DR   RefSeq; XP_015641765.1; XM_015786279.1.
DR   AlphaFoldDB; Q5Z6G5; -.
DR   SMR; Q5Z6G5; -.
DR   STRING; 4530.OS06T0697200-00; -.
DR   PaxDb; Q5Z6G5; -.
DR   PRIDE; Q5Z6G5; -.
DR   EnsemblPlants; Os06t0697200-00; Os06t0697200-00; Os06g0697200.
DR   GeneID; 4341945; -.
DR   Gramene; Os06t0697200-00; Os06t0697200-00; Os06g0697200.
DR   KEGG; osa:4341945; -.
DR   eggNOG; KOG0341; Eukaryota.
DR   HOGENOM; CLU_003041_16_5_1; -.
DR   InParanoid; Q5Z6G5; -.
DR   OMA; GHRIEAC; -.
DR   OrthoDB; 447398at2759; -.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000007752; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   Genevisible; Q5Z6G5; OS.
DR   GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   CDD; cd17951; DEADc_DDX41; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR044113; DEADc_DDX41.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Metal-binding; Nucleotide-binding;
KW   Reference proteome; RNA-binding; Zinc; Zinc-finger.
FT   CHAIN           1..619
FT                   /note="DEAD-box ATP-dependent RNA helicase 35B"
FT                   /id="PRO_0000282499"
FT   DOMAIN          204..388
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          399..559
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   ZN_FING         576..593
FT                   /note="CCHC-type"
FT   REGION          1..72
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           173..201
FT                   /note="Q motif"
FT   MOTIF           336..339
FT                   /note="DEAD box"
FT   COMPBIAS        16..46
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         217..224
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   619 AA;  68144 MW;  EF173A61AF86EF16 CRC64;
     MAAAAAAAAA GDSPRRGTPD SDEEDYEEYV PVAKRRAMEA ERLRRATKPP TTNAVAVAAP
     PPPPRSTSSP AVGEVAVKTS LLVKATKLKR EAPEVTPAER LLQQEREMIE HLSDRKALMP
     VGEIAKGISY SEPITTGWRP PLRLRRMPRS RADALRRSWH ILVDGDDVPP PSRSFGDLRL
     PEPILRALRG KGIEKPTPIQ VQGLPVALSG RDMIGIAFTG SGKTLVFVLP LIMAALQEEI
     LMPIVPGEGP FGLIVCPSRE LARQTHEVIE MFLAPLMEAG YPEIRPLLCI GGVDMRTQME
     VVKKGVHIVV ATPGRLKDLL SKKKMNLDNC RYLTLDEADR LVDLGFEDDI REVFDHFKAQ
     RQTLLFSATM PEKIQNFAKS ALVKPIIVNV GRAGAANLDV IQEVEYVKEE ARIIYLLECL
     QKTPPPVLVF CEHKADVDYI QEFLLLKGVE AVAIHGGKDD EERKDAFKSF KASEKDVLVA
     TDVASKGLDI PDIQHVINYD MPAEIENYVH RIGRTGRRGK TGVATTFINK NQTETTLLDL
     KQLLIESKQR LPPILADLDD PQEDDKVAIA QQSGVKGCAF CGGLGHRIEA CPKQQLQNSV
     TLARARSDYF GGGGYRGEI