ID   RH35_ARATH              Reviewed;         591 AA.
AC   Q9LU46; Q0WWH1;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 136.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase 35;
DE            EC=3.6.4.13;
GN   Name=RH35; OrderedLocusNames=At5g51280; ORFNames=MWD22.23;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT   features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:31-63(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17168887; DOI=10.1111/j.1467-7652.2004.00084.x;
RA   Mingam A., Toffano-Nioche C., Brunaud V., Boudet N., Kreis M., Lecharny A.;
RT   "DEAD-box RNA helicases in Arabidopsis thaliana: establishing a link
RT   between quantitative expression, gene structure and evolution of a family
RT   of genes.";
RL   Plant Biotechnol. J. 2:401-415(2004).
RN   [5]
RP   GENE FAMILY.
RX   PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA   Xu R., Zhang S., Huang J., Zheng C.;
RT   "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT   in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT   sativa.";
RL   PLoS ONE 8:E78982-E78982(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC       family of RNA helicases and controls ATP binding and hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX41 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AB023044; BAA97391.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED96061.1; -; Genomic_DNA.
DR   EMBL; AK226380; BAE98527.1; -; mRNA.
DR   RefSeq; NP_199941.1; NM_124507.5.
DR   AlphaFoldDB; Q9LU46; -.
DR   SMR; Q9LU46; -.
DR   BioGRID; 20447; 5.
DR   IntAct; Q9LU46; 1.
DR   STRING; 3702.AT5G51280.1; -.
DR   PaxDb; Q9LU46; -.
DR   PRIDE; Q9LU46; -.
DR   ProteomicsDB; 236961; -.
DR   DNASU; 835202; -.
DR   EnsemblPlants; AT5G51280.1; AT5G51280.1; AT5G51280.
DR   GeneID; 835202; -.
DR   Gramene; AT5G51280.1; AT5G51280.1; AT5G51280.
DR   KEGG; ath:AT5G51280; -.
DR   Araport; AT5G51280; -.
DR   TAIR; locus:2176192; AT5G51280.
DR   eggNOG; KOG0341; Eukaryota.
DR   HOGENOM; CLU_003041_16_5_1; -.
DR   InParanoid; Q9LU46; -.
DR   OMA; DMLDKKM; -.
DR   OrthoDB; 447398at2759; -.
DR   PhylomeDB; Q9LU46; -.
DR   PRO; PR:Q9LU46; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LU46; baseline and differential.
DR   Genevisible; Q9LU46; AT.
DR   GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   CDD; cd17951; DEADc_DDX41; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR044113; DEADc_DDX41.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   InterPro; IPR001878; Znf_CCHC.
DR   InterPro; IPR036875; Znf_CCHC_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF00098; zf-CCHC; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00343; ZnF_C2HC; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF57756; SSF57756; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
DR   PROSITE; PS50158; ZF_CCHC; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Helicase; Hydrolase; Metal-binding; Nucleotide-binding;
KW   Reference proteome; RNA-binding; Zinc; Zinc-finger.
FT   CHAIN           1..591
FT                   /note="DEAD-box ATP-dependent RNA helicase 35"
FT                   /id="PRO_0000239175"
FT   DOMAIN          177..361
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          372..532
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   ZN_FING         548..565
FT                   /note="CCHC-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT   MOTIF           146..174
FT                   /note="Q motif"
FT   MOTIF           309..312
FT                   /note="DEAD box"
FT   BINDING         190..197
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   591 AA;  65806 MW;  3D48EC520A775435 CRC64;
     MESIMEEADS YIEYVSVAER RAIAAQKILQ RKGKASELEE EADKEKLAEA KPSLLVQATQ
     LKRDVPEVSA TEQIILQEKE MMEHLSDKKT LMSVRELAKG ITYTEPLLTG WKPPLHIRKM
     SSKQRDLIRK QWHIIVNGDD IPPPIKNFKD MKFPRPVLDT LKEKGIVQPT PIQVQGLPVI
     LAGRDMIGIA FTGSGKTLVF VLPMIMIALQ EEMMMPIAAG EGPIGLIVCP SRELARQTYE
     VVEQFVAPLV EAGYPPLRSL LCIGGIDMRS QLEVVKRGVH IVVATPGRLK DMLAKKKMSL
     DACRYLTLDE ADRLVDLGFE DDIREVFDHF KSQRQTLLFS ATMPTKIQIF ARSALVKPVT
     VNVGRAGAAN LDVIQEVEYV KQEAKIVYLL ECLQKTSPPV LIFCENKADV DDIHEYLLLK
     GVEAVAIHGG KDQEDREYAI SSFKAGKKDV LVATDVASKG LDFPDIQHVI NYDMPAEIEN
     YVHRIGRTGR CGKTGIATTF INKNQSETTL LDLKHLLQEA KQRIPPVLAE LNDPMEEAET
     IANASGVKGC AYCGGLGHRI RDCPKLEHQK SVAISNSRKD YFGSGGYRGE I